Introduction to Membrane Protein
Membrane proteins are an integral part of molecular biology research. These proteins interact with biological membranes and are the target of over 50% of all modern medicinal drugs. Membrane proteins perform a variety of functions that are crucial to the survival of organisms such as relaying signals, transport, and cell adhesion. Additionally, approximately 20 – 30 % of genes within a genome encode membrane proteins.
At BioChain, we’ve prepared membrane proteins from a large variety of documented quality tissues that are ready-to-use for genetic testing and analysis. Our tissue membranes are prepared from whole tissue homogenates and are of high-quality. Distinction of quality is ensured by consistent patterns on SDS-PAGE analysis, as well as by storage in protease inhibitor cocktail buffer.
FeaturesMembrane protein isolated from diseased and normal tissues and from tumor cell lines
Available from human, monkey and rat tissues
Decontamination of polysaccharide, proteoglycan, RNase, and genomic DNA
Extensive quality control procedures to ensure high quality
Documentation of tissues' clinical history is available
ApplicationsWestern Blot (10 µg to 20 µg per lane is recommended for a mini gel)
Enzymatic activity analysis
Tissue specific expression
Protein lysate is verified on SDS-PAGE analysis as a consistent pattern.
Protein lysate is positively confirmed by Western Blot using specific biomarker: GAPDH for total protein, cytoplasmic and universal total protein; Histone for nuclear protein; Na/K ATPase for membrane protein; Vimentin for cytoskeleton protein.