United States
Matched Pair - Total Protein
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Matched Pair - Total Protein

Introduction to Matched Pair - Total Protein

BioChain’s human matched pair total protein products have been generated from human tumors and the corresponding normal tissues from the same donor. BioChain offers Primary Pair (PP), as well as Primary and Metastatic Pair (PM) products. Primary Pair total proteins are those that have been isolated from primary tumors and its adjacent unaffected normal tissue. Primary Metastatic Pair total proteins have been isolated from primary tumors and corresponding metastatic tumors. Total protein is isolated by our proprietary techniques and stored in protein storage buffer. This product is a useful tool for accelerating gene expression analysis by identifying tumor-specific and tumor metastatic proteins and genes.

Features

  • Total protein isolated from tumor and normal tissues from the same patient
  • Decontamination of polysaccharide, proteoglycan, RNase, and genomic DNA
  • Extensive quality control procedures to ensure high quality
  • Documentation of tissues' clinical history is available
  • Applications

  • Western Blot (10 µg to 20 µg per lane is recommended for a mini gel).
  • Immunoprecipitation
  • Electrophoresis
  • Enzymatic activity analysis
  • Protein-protein interaction
  • Tissue specific expression
  • Quality Control

  • Protein lysate is verified on SDS-PAGE analysis as a consistent pattern.
  • Protein lysate is positively confirmed by Western Blot using specific biomarker: GAPDH for total protein, cytoplasmic and universal total protein; Histone for nuclear protein; Na/K ATPase for membrane protein; Vimentin for cytoskeleton protein.
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