United States


Biospecimen Protein, Purification & Expression

Introduction to Biospecimen Protein, Purification & Expression

BioChain has gained the reputation as a unique and quality biospecimen provider. Proteins are the functional units of all forms of life. Our products in this area, such as Universal Protein Lysate, Compartmental Protein, Matched Pair - Total Protein, Mega Western Protein Array, Mitochondria Isolation Kit, Protein Analysis, and Protein Extraction, are the favorites for our customers throughout the history of our company.

 

    Total Protein

BioChain's total protein product is one of the most popular products on the market for investigating protein expression among different tissues. Total amount and portion of proteins are common materials for researchers studying protein expression. Total proteins are isolated from approximately 200 different human adult and fetal normal tissues, human diseased and tumor tissues, as well as from mouse, rat, monkey, and plant tissues. 

• Human Adult Normal Protein            
• Human Fetal Normal Protein             
• Human Diabetes Protein             
• Human Tumor Protein             
• Human Tumor Cell Line Protein
• Human Heart Disease Protein
• Human Lung Disease Protein
• Human Liver Cirrhosis Protein
• Human Lupus Protein
• Human Neurological Disease Protein
• Monkey (Cynomolgus) Normal Protein
• Monkey (Rhesus) Normal Protein
• Mouse Normal Protein
• Rat Normal Protein
• Porcine Normal Protein
• Rabbit Normal Protein
• Plant Normal Protein


 

    Membrane Protein 

Tissue membrane protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis. The protein is stored in a buffer with protease inhibitor cocktail. 

• Membrane Protein (Subcellular) 


 

    Universal Protein Lysate

BioChain's Universal Protein Lysate is the total protein isolated from a variety of tissue sources including human, monkey, dog, mouse, and rat, to ensure the broadest coverage of proteins. It can be used as a control for comparison of protein expression and antibody microarray study. Our product is manufactured on an industrial scale to minimize lot-to-lot variation. 

• Human Normal Universal Protein Lysate             
• Monkey Normal Universal Protein Lysate             
• Mouse Normal Universal Protein Lysate             
• Rat Normal Universal Protein Lysate            
• Dog Normal Universal Protein Lysate            
• Chicken Normal Universal Protein Lysate             


 

    Protein Analysis

BioChain provides a variety of reagents for protein analysis, such as Western blot, immunohistochemistry, and protein profiling. These kits enable scientists to analyze protein expression in protein lysate and in tissue sections. 

• Attoglow Western Blot Analysis Kit             
• Attoglow Advance Western Blot Analysis Kit             
• DAB Substrate Kit             
• FastRed Substrate Kit             
• Immuno HistoChemistry (IHC) Kit            
• Western Blot Enhancing Kit             


 

    Mitochondria Isolation Kit

BioChain's mitochondria Isolation kit is designed to isolate mitochondria which are maintained as intact and active. Mitochondria are the eukaryotic subcellular organelles which contain the enzymes of the citric acid cycle, the electron transport chain, and oxidative phosphorylation. As new discoveries of human disorders are being related to mitochondrial dysfunction, there is an increasing need for an effective method to isolate intact mitochondria from tissues and cultured cells. The key steps when isolating mitochondria are: (i) rupturing of cells by mechanical and/or chemical means and (ii) differential centrifugation at low speed to remove debris and extremely large cellular organelles followed by centrifugation at a higher speed to isolate mitochondria. 

• Mitochondria Isolation Kit for Tissue and Cultured Cells


Protein Purification & Expression
 

    Total Protein Western Blots 

BioChain's total protein Western Blots are specifically designed for proteomics, gene discovery, and functional analysis. All products are manufactured using high quality proteins from a wide variety of human adult and fetal normal tissues, human diseased and tumor tissues, mouse, rat, monkey, and plant tissues. The Total Protein Western Blots are suitable for both radioactive and non-radioactive detection.                                                

• Human Adult Normal Blot            
• Human Fetal Normal Blot             
• Human Tumor Different Type Different Donor Blot             
• Human Tumor Same Type Different Donor Blot             
• Human Tumor Cell Line Blot            
• Monkey (Cynomolgus) Normal Blot             
• Monkey (Rhesus) Normal Blot             
• Mouse Normal Blot            
• Rat Normal Blot            


 

    Matched Pair Total Protein 

BioChain's human total protein matched pair products include: Primary Pair (PP) and Primary and Metastatic Pair (PM). PP consists of total protein isolated from primary tumor and its adjacent normal tissue; PM consists of total protein from primary tumor and corresponding metastatic tumor. Total proteins in each pair are prepared from the same donor. This product line is designed for identifying tumor-specific proteins/genes and tumor metastatic proteins/genes.                                           

• Human Tumor Matched Pair Total Protein          


 

    Protein Extraction 

BioChain is a leading provider of protein lysates extracted from hundreds of different tissues and cells. BioChain also offers simple and reliable kits to our customers which provide tools for protein lysate extraction.

• Total Protein Extraction Kit             
• Membrane Protein Extraction Kit             
• CNMCS Compartmental Protein Extraction Kit             
• CNM Compartmental Protein Extraction Kit          


 

    Mega Western Protein Array

BioChain's Mega Western Protein Array is a novel tool for analysis of protein expressions by combining the unique features of tissue protein micro-arrays and Western blots. The dimensions of Mega Western Protein Array are 1x2 inches, and the detection method is the same as one that is commonly employed for Western blot analysis. Using the Mega Western Protein Array, protein expression levels and protein sizes can be simultaneously determined in more than 30 different tissues, a unique feature which is not achievable by regular Western blot.                            

• Human Adult Normal Mega Western Protein Array
• Multiple Species Mega Western Protein Arrays