Introduction to Universal cDNA
BioChain’s Universal cDNAs are premade first strand cDNAs that can be used to amplify a gene of interest by using gene-specific primers. Our Univeral cDNAs are an ideal tool for amplifying previously isolated, structurally related, or cross-species cDNAs. Use this product to directly amplify a cDNA of interested while avoiding traditional library construction and screening. Universal cDNA serves as a standard for comparison of gene expression by real time PCR and regular PCR, and also as a gene pool for cloning genes.
In order to obtain good results from PCR amplification a high-quality cDNA template is needed. Our Universal cDNAs are synthesized from our premium, high-quality RNA that is DNA-free, and is reverse transcribed using random hexamer or oligo dT primers.