cDNA Synthesis Kit(Reverse Transcription)
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|Optimax First Strand cDNA Synthesis Kit|| 100 rnx||
Introduction to cDNA Synthesis Kit(Reverse Transcription)
BioChain’s First Strand cDNA Synthesis Kit provides all the necessary components to perform 100 first strand cDNA synthesis reactions from total RNA or poly(A) RNA. This system uses Moloney Murine Leukemia Virus Reverse Transcriptase with lower RNase H activity to allow for the synthesis of full-length cDNA from long templates. Oligo(dT) 18 and random hexamer primers are included, however, other specific primers can also be used with this kit.
The cDNA synthesized using this system can be used directly in PCR amplification. This kit is has been optimized for maximum yield of full-length cDNA. Ribonuclease inhibitor has also been added to lower the risk of mRNA degradation during the reaction.
Features Full-length, first strand cDNA synthesis
Ready for PCR amplification
Templates for second strand synthesis and construction of cDNA libraries
Complete system with positive control primers and BioChain’s premium quality human placenta total RNA included
Applications Immediate PCR amplification of known genes
Comparison of gene expression patterns between biological samples
Gene mutation analysis
Gene cloning and target sequencing