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The recombinant HIV antigen is coated on the multiple wells. When the serum sample and HIVAg labeled with HRP (conjugated) are added to the coated wells, and if Anti-HIV is present in the sample, a complex of HIVAg-Anti-HIV-HIVAg will form. This enzyme reaction produces a color change, and the intensity of the absorbance at 450 nm indicates the presence or absence of the Anti-HIV in the sample. The test is specific, sensitive, reproducible and easy to operate. It is of vital importance in HIV diagnosis and blood screen.
Microtitration wells coated with murine anti-HIV-1 P24 capture antibody, are exposed to test specimens, which may contain p24 reactive determinants. After an incubation period, unbound components in the test sample are washed away. Specifically bound p24 reactive determinants react with a mouse anti-HIV-1 p24 conjugated with biotin during a second incubation period. Following a second wash cycle the biotinylated antibody is detected by the addition of a streptavidin HRP conjugate. Following a third wash cycle, specifically bound enzyme conjugate is detected by reaction with the Substrate solution, tetramethylbenzidine (TMB). The assay is measured spectrophotometrically to indicate the level of p24 reactive determinants present in a sample.
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