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Introduction to
mRNA
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BioChain's mRNA is isolated from total RNA obtained from human adult and fetal normal tissues, human tumor and diseased tissues, as well as from mouse, rat, monkey, and plant tissues. The RNA undergoes two rounds of oligo(dT) selection utilizing BioChain's proprietary technology to minimize the presence of ribosomal RNA. Potential DNA contaminants are removed by a stringent DNase treatment. The purity and intactness of the mRNA is verified by agarose gel electrophoresis, Northern analysis, cDNA synthesis, and capillary electrophoresis using an Agilent 2100 bioanalyzer. More...
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Features
(View Images)
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mRNA isolated from a wide variety of tissues and cells
DNase I treatment to ensure absence of genomic DNA
RNA lengths can reach over 12 kb, indicating the intactness
High efficiency reverse transcription
Representative of total RNA
Documentation of tissues' clinical histories is available
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Applications |
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Northern Blotting, RT-PCR, and RACE
cDNA synthesis and cDNA library construction
cDNA probe for profiling study in gene expression
RNA protection and primer extension
RNA display and reverse Northern blotting
In vitro translation
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Description |
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The mRNA is isolated from a variety of human normal adult, fetel, tumor, or mouse, rat and monkey tissues. The total RNA used for purification of mRNA is isolated by our proprietary techniques, including treatment with RNA selective reagents for ultrapure RNA. Contamination by RNase, DNA polysaccharides and proteoglycans has been effectively eliminated. The mRNA is purified by affinity chromatography using oligo dT culumns. The integrity of our mRNA is checked on a denaturing agarose gel followed by Northern analysis using a human ?-actin cDNA probe.
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Storage Conditions |
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Store mRNA at -70°C
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