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Introduction to
cDNA Library
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Making cDNA libraries is an art as well as science. It takes great skills, knowledge, as well as experience to make high quality cDNA libraries. The cDNA libraries provided by BioChain are subjected to rigorous quality control testing to ensure they are of the highest quality. More...
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Features
(View Images)
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6 minimum numbers of primary clones, and 3x106 average numbers of primary clones
At least 87% of recombinant clones, and 95% average of recombinant clones
Insert size at least 1 kb, and average insert size 1.5 kb
Normalized cDNA libraries
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Applications |
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Conventional library screening
PCR amplification
cDNA expression
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Description |
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Our high quality cDNA libraries are constructed by using an oligo dT primer-adapter and Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) to prime and synthesize first strand cDNA from mRNA. After the second strand is synthesized, the double stranded cDNA is size fractionated, cloned directionally into our BioExpress vector and transformed into T1 phage resistant E. coli. The 4 kb BioExpress vector used for cloning is Puc based, confers ampicillin resistance and contains the CMV promoter for expression analysis. This vector also contains the SP6 and T7 RNA polymerase promoters flanking the MCS for RNA synthesis, the Amersham ET and M13 primer sites for sequencing and the F1 ori for single-stranded DNA production. By cloning the cDNA directionally into this vector the cDNA clones can be expressed, detected by antibody screening. We provide both general and normalized cDNA libraries.
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Storage Conditions |
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Store the cDNA library at -70°C
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