Promotions

mRNA:

• Jacob Glanville, Wenwu Zhai, Jan Berka, Dilduz Telman, Gabriella Huerta, Gautam R. Mehta, Irene Ni, Li Mei, Purnima D. Sundar, Giles M. R. Day, David Cox, Arvind Rajpal, and Jaume Pons
  Precise determination of the diversity of a combinatorial antibody library gives insight into the human immunoglobulin repertoire
  PNAS, Dec 2009; 106: 20216 - 20221.
  ... ...Total RNA and/or mRNA were obtained from 637 healthy human peripheral blood leukocyte donors (BioChain Institute and Clontech) and 17 human spleens (BioChain Institute, Clontech, and OriGene). First strand cDNA was synthesized by using human heavy... ...
  Ref. link
  


• Henning Kessler, Angelika Herm-Götz, Stephan Hegge, Manuel Rauch, Dominique Soldati-Favre, Friedrich Frischknecht, and Markus Meissner
  Microneme protein 8 ¨C a new essential invasion factor in Toxoplasma gondii
  J. Cell Sci., Apr 2008; 121: 947 - 956.
  ... ...of MIC8, MIC8-like1 and MIC8-like2 was confirmed by RT-PCR (Titan one tube RT-PCR; Roche) on mRNA (isolated with mRNAeasy; Biochain) using oligonucleotide pairs MIC8-s (5-GCGGCAATTGCATTGGTGGTTGTGG-3), MIC8as (5-GCCTTAATTAAGACGCGATACCGCCCGAAGG-3), MIC8/2-s... ...
  Ref. link
  


• Cédric Duret, Sabine Gerbal-Chaloin, Jeanne Ramos, Jean-Michel Fabre, Eric Jacquet, Francis Navarro, Pierre Blanc, Antonio Sa-Cunha, Patrick Maurel, and Martine Daujat-Chavanieu
  Isolation, Characterization, and Differentiation to Hepatocyte-Like Cells of Nonparenchymal Epithelial Cells from Adult Human Liver
  Stem Cells, Jul 2007; 25: 1779 - 1790.
  ... ...HNF4alpha]), or from 1 to 1/1,000 (for other genes) of a pool of mRNA isolated from human fetal (BioChain Institute, Hayward, CA, http://www.biochain.com ) (for glutathione S-transferase pi [GSTpi] and cytokeratins 7 and 19) or adult (for the other... ...
  Ref. link
  


• Cedric Duret, Sabine Gerbal-Chaloin, Jeanne Ramos, Jean-Michel Fabre, Eric Jacquet, Francis Navarro, Pierre Blanc, Antonio Sa-Cunha, Patrick Maurel, and Martine Daujat-Chavanieu
  ISOLATION, CHARACTERIZATION AND DIFFERENTIATION TO HEPATOCYTE-LIKE CELLS OF NON PARENCHYMAL EPITHELIAL CELLS FROM ADULT HUMAN LIVER
  Stem Cells, Apr 2007; 10.1634/stemcells.2006-0664.
  ... ...1/5000 (for hepatocyte nuclear factors HNF4) or 1 to 1/1000 (for other genes) of a pool of mRNA isolated from human fetal (Biochain Institute,Inc,USA) (for GST, cytokeratins 7 and 19) or adult hepatocytes (for the other genes). The expression of 18S RNA was... ...
  Ref. link
  


• Jae-Hyun Park, Meng-Lay Lin, Toshihiko Nishidate, Yusuke Nakamura, and Toyomasa Katagiri
  PDZ-Binding Kinase/T-LAK Cell-Originated Protein Kinase, a Putative Cancer/Testis Antigen with an Oncogenic Activity in Breast Cancer
  Cancer Res., Sep 2006; 66: 9186 - 9195.
  ... ...of mRNA isolated from normal adult human mammary gland (Biochain, Hayward, CA), lung, heart, liver, kidney, and bone marrow...tissue sections of heart, lung, liver, kidney, and testis (Biochain). Briefly, paraffin-embedded specimens were treated with... ...
  Ref. link
  


• Tayebeh Rezaie, David M. Waitzman, Jennifer L. Seeman, Paul L. Kaufman, and Mansoor Sarfarazi
  Molecular Cloning and Expression Profiling of Optineurin in the Rhesus Monkey
  Invest. Ophthalmol. Vis. Sci., Jul 2005; 46: 2404 - 2410.
  ... ...normalized by amount of mRNA) blot from Biochain Institute (Hayward, CA) was used...prehybridized (FastHyb solution; Biochain) for 3 hours at 65C and subsequently...of an mRNA multiple tissue blot (BioChain). As shown in Figure 3 , two different... ...
  
  
• Xiaomin Zhang, Gohar Azhar, Ying Zhong, and Jeanne Y. Wei
  Identification of a Novel Serum Response Factor Cofactor in Cardiac Gene Regulation
  J. Biol. Chem., Dec 2004; 279: 55626 - 55632.
  ... The human heart mRNA samples were obtained from Biochain Institute (Hayward, CA). ... 
  


• Soji Kakiuchi, Yataro Daigo, Nobuhisa Ishikawa, Chiyuki Furukawa, Tatsuhiko Tsunoda, Seiji Yano, Kazuhiko Nakagawa, Takashi Tsuruo, Nobuoki Kohno, Masahiro Fukuoka, Saburo Sone, and Yusuke Nakamura
  Prediction of sensitivity of advanced non-small cell lung cancers to gefitinib (Iressa, ZD1839)
  Hum. Mol. Genet., Dec 2004; 13: 3029 - 3043.
  ... probe, normal human lung poly(A) + RNA (BD Biosciences Clontech, Palo Alto, CA, USA and BIOCHAIN, Hayward, CA, USA) was amplified in the same way. ...
  
  
• Vidya Subramanian, Alexis Rothenberg, Carlos Gomez, Alex W. Cohen, Anne Garcia, Sucharita Bhattacharyya, Lawrence Shapiro, Rong Wang, Michael P. Lisanti, and Dawn L. Brasaemle
  Perilipin A mediates the reversible binding of CGI-58 to lipid droplets in 3T3-L1 adipocytes
  J. Biol. Chem., Aug 2004; 10.1074/jbc.M407462200.
  ... (Rockville, MD) and BioChain Institute, Inc. ...
  
  ... Nitrocellulose membranes containing poly A mRNA from mouse tissues (OriGene Technologies, Inc., Rockville, MD and BioChain Institute, Inc., Hayward, CA) were probed with 32 P-labeled cDNA probes for murine CGI-58. ...
  


• Hideto Jinno, Toshiko Tanaka-Kagawa, Akiko Ohno, Yuko Makino, Erika Matsushima, Nobumitsu Hanioka, and Masanori Ando
  Functional Characterization of Cytochrome P450 2B6 Allelic Variants
  Drug Metab. Dispos., Apr 2003; 31: 398 - 403.
  ...Human adult normal liver mRNA was purchased from BioChain Institute Hayward, CA. ...
  
  
• Abe S, Katagiri T, Saito-Hisaminato A, Usami SI, Inoue Y, Tsunoda T, Nakamura Y.
  Identification of CRYM as a Candidate Responsible for Nonsyndromic Deafness, through cDNA Microarray Analysis of Human Cochlear and Vestibular Tissues
  Am J Hum Genet. 2003 Jan; 72(1): 73-82. published online before print December 6, 2002
  ... ...We obtained 70?0 µg of each amplified RNA (aRNA) sample. As a control, we mixed PolyA(+) RNAs derived from 29 normal human tissues (bone marrow, brain, heart, kidney, liver, lung, lymph node, mammary gland, pancreas, placenta, prostate, salivary gland, skeletal muscle, small intestine, spinal cord, spleen, stomach, testis, thymus, thyroid, trachea, uterus, fetal brain, fetal kidney, fetal liver, fetal lung [Clontech], colon, ovary [Biochain], and mesenteric adipose tissue). ... ...
  Ref. link
  


• Ramesh Ramakrishnan, David Dorris, Anna Lublinsky, Allen Nguyen, Marc Domanus, Anna Prokhorova, Linn Gieser, Edward Touma, Randall Lockner, Murthy Tata, Xiaomei Zhu, Marcus Patterson, Richard Shippy, Timothy J. Sendera, and Abhijit Mazumder
  An assessment of Motorola CodeLink^TM microarray performance for gene expression profiling applications
  Nucleic Acids Res., Apr 2002; 30: 30.
  ... MATERIALS AND METHODS Target preparation Five micrograms of total RNA (BioChain, Hayward, CA) were added to a reaction mix in a final volume of 12 µl, ...
  ... compared within three human poly(A)+ RNA samples (heart lot#A403084, brain lot#A311144 and kidney lot#A404013 from BioChain) using the Motorola CodeLink(TM) UniSet Human 1 expression bioarrays and the ABI TaqMan (R) products. ...
  


• Jian Jin, Xuehui Chen, Yan Zhou, Mark Bartlam, Qing Guo, Yiwei Liu, Yixin Sun, Yu Gao, Sheng Ye, Guangtao Li, Zihe Rao, Boqin Qiang, and Jiangang Yuan
  Crystal structure of the catalytic domain of a human thioredoxin-like protein: Implications for substrate specificity and a novel regulation mechanism
  Eur. J. Biochem., Apr 2002; 269: 2060 - 2068.
  ... DDRT-PCR and full-length cDNA isolation Total RNA (2.5 µg) from 13- and 33-week-old human fetal cerebrum (Biochain) was reverse transcribed by Superscript II (Gibco-BRL) using a single-base anchored 3′ primer (5′-AAGCTTTTTTTTTTTN-3′, N=C, G, ...
  ... Northern blot analysis Total Poly(A) + RNA from 13- and 33-week-old human fetal cerebrum (Biochain) was electrophoresed in a 1% agarose gel containing 0.66 M formaldehyde and was blotted onto a ...
  


• Byung-Taek Kim, Hiroshi Kitagawa, Jun-ichi Tamura, Toshiyuki Saito, Marion Kusche-Gullberg, Ulf Lindahl, and Kazuyuki Sugahara
  Human tumor suppressor EXT gene family members EXTL1 and EXTL3 encode 1,4- N-acetylglucosaminyltransferases that likely are involved in heparan sulfate/ heparin biosynthesis
  PNAS, Jun 2001; 98: 7176 - 7181. 
  ... was amplified with the first-strand cDNA transcribed from human adult brain poly (A) + RNA (Biochain Institute, San Leandro, CA) as a template by PCR by using a 5′ primer (5'-ATTGATCACATGGTGGCAGCTGCAACTG-3').
  


• Huibin Yue, P. Scott Eastman, Bruce B. Wang, James Minor, Michael H. Doctolero, Rachel L. Nuttall, Robert Stack, John W. Becker, Julie R. Montgomery, Marina Vainer, and Rick Johnston
  An evaluation of the performance of cDNA microarrays for detecting changes in global mRNA expression
  Nucleic Acids Res., Apr 2001; 29: 41.
  ... Oligotex resin (Qiagen, Valencia, CA) from commercially available human placenta, brain and heart total RNA (Biochain, San Leandro, CA). ...
  ... done in the presence of 200 ng poly(A) mRNA, from either human brain or heart (Biochain, Hayward, CA). ...
  


• Chang Bai, Brett Connolly, Michael L. Metzker, Catherine A. Hilliard, Xiaomei Liu, Volker Sandig, Avery Soderman, Sheila M. Galloway, Qingyun Liu, Christopher P. Austin, and C. Thomas Caskey
  Overexpression of M68/DcR3 in human gastrointestinal tract tumors independent of gene amplification and its location in a four-gene cluster
  PNAS, Feb 2000; 97: 1230 - 1235. 
  ... Northern Analysis. mRNA isolated from human tumor samples was obtained from BioChain Institute, San Leandro, CA. ...
  


• Adrienne E. Dubin, Rene Huvar, Michael R. D'Andrea, Jayashree Pyati, Jessica Y. Zhu, K. C. Joy, Sandy J. Wilson, Jose E. Galindo, Charles A. Glass, Lin Luo, Michael R. Jackson, Timothy W. Lovenberg, and Mark G. Erlander
  The Pharmacological and Functional Characteristics of the Serotonin 5-HT_3A Receptor Are Specifically Modified by a 5-HT_3B Receptor Subunit
  J. Biol. Chem., Oct 1999; 274: 30799 - 30810. 
  ... Total RNA from normal human ascending and descending colon (CDP-061068; lot 8906066; CDP-061069; lot 8906067; BioChain Institute, Inc.) and 5 µg of each poly(A) RNA from normal human small intestine and ...
  


• Henry M. Sarau, Robert S. Ames, Jon Chambers, Catherine Ellis, Nabil Elshourbagy, James J. Foley, Dulcie B. Schmidt, Roseanna M. Muccitelli, Owen Jenkins, Paul R. Murdock, Nicole C. Herrity, Wendy Halsey, Ganesh Sathe, Alison I. Muir, Parvathi Nuthulaganti, George M. Dytko, Peter T. Buckley, Shelagh Wilson, Derk J. Bergsma, and Douglas W.P. Hay
  ACCELERATED COMMUNICATION: Identification, Molecular Cloning, Expression, and Characterization of a Cysteinyl Leukotriene Receptor
  Mol. Pharmacol., Sep 1999; 56: 657 - 663. 
  ...poly A+ RNA from multiple tissues of four different individuals (two males, two females, except prostate) was prepared, (Biochain, San Leandro, CA; Clontech, Palo Alto, CA; Invitrogen, Leek, the Netherlands; Analytical Biological Services, Wilmington, ...