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Reference
mRNA Northern Blots RNAmate
Total RNA RNA Array cDNA
Protein Tissue Section Tissue Array
Genomic DNA Antibody Kit & Reagent
Others All References


Total RNA:

  • Scott J. Diede, Jamie Guenthoer, Linda N. Geng, Sarah E. Mahoney, Michael Marotta, James M. Olson, Hisashi Tanaka, and Stephen J. Tapscott
     DNA methylation of developmental genes in pediatric medulloblastomas identified by denaturation analysis of methylation differences
     PNAS, Dec 2009; 10.1073/pnas.0907606106.
    ... ...manufacturer's pro- tocol (Invitrogen) and quantitated using a NanoDrop spec- trophotometer. Normal cerebellum RNA was obtained from Biochain. First-strand cDNA synthesis was performed using random hexamers on 1 g of total RNA as input, using the Su- perScript III... ...
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  • Jacob Glanville, Wenwu Zhai, Jan Berka, Dilduz Telman, Gabriella Huerta, Gautam R. Mehta, Irene Ni, Li Mei, Purnima D. Sundar, Giles M. R. Day, David Cox, Arvind Rajpal, and Jaume Pons
     Precise determination of the diversity of a combinatorial antibody library gives insight into the human immunoglobulin repertoire
     PNAS, Dec 2009; 106: 20216 - 20221.
    ... ...Total RNA and/or mRNA were obtained from 637 healthy human peripheral blood leukocyte donors (BioChain Institute and Clontech) and 17 human spleens (BioChain Institute, Clontech, and OriGene). First strand cDNA was synthesized by using human heavy... ...
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  • Aurélie Ernst, Stefanie Hofmann, Rezvan Ahmadi, Natalia Becker, Andrey Korshunov, Felix Engel, Christian Hartmann, Jörg Felsberg, Michael Sabel, Heike Peterziel, Moritz Durchdewald, Jochen Hess, Sebastian Barbus, Benito Campos, Anna Starzinski-Powitz, Andreas Unterberg, Guido Reifenberger, Peter Lichter, Christel Herold-Mende, and Bernhard Radlwimmer
     Genomic and Expression Profiling of Glioblastoma Stem Cell–Like Spheroid Cultures Identifies Novel Tumor-Relevant Genes Associated with Survival
     Clin. Cancer Res., Nov 2009; 15: 6541 - 6550.
    ... ...candidate genes in tumors, a reference of total RNA obtained from nonneoplastic human brain tissue samples of five individuals (BioChain) was used. Total RNA was treated with DNaseI (Invitrogen) and reverse-transcribed with SuperscriptII (Invitrogen). Each cDNA... ...
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  • Bin Ye, Stacie L. Kroboth, Jie-Lin Pu, Jason J. Sims, Nitin T. Aggarwal, Elizabeth M. McNally, Jonathan C. Makielski, and Nian-Qing Shi
     Molecular Identification and Functional Characterization of a Mitochondrial Sulfonylurea Receptor 2 Splice Variant Generated by Intraexonic Splicing
     Circ. Res., Nov 2009; 105: 1083 - 1093.
    ... ...performed using a mouse or human heart FirstChoice RACE-Ready cDNA library (Amnion, Austin, Tex). Human LV RNA was obtained from Biochain (Hayward, Calif). Mouse heart RNA was extracted using the TRIzol reagent (Invitrogen, Carlsbad, Calif). RT-PCRs were carried... ...
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  • Dilini Ranatunga, Christian M. Hedrich, Fengying Wang, Daniel W. McVicar, Nathan Nowak, Trupti Joshi, Lionel Feigenbaum, Lindsay R. Grant, Simona Stäger, and Jay H. Bream
     A human IL10 BAC transgene reveals tissue-specific control of IL-10 expression and alters disease outcome
     PNAS, Oct 2009; 106: 17123 - 17128.
    ... ...a first strand cDNA synthesis kit (Invitrogen). Total human RNA isolated from tissues of healthy donors was purchased from BioChain Institute, Inc. Quantitative PCR was performed using Taqman site-specific primers and probes (Applied Biosystems) on an ABI... ...
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  • Dimitrios Iliopoulos, Christos Polytarchou, Maria Hatziapostolou, Filippos Kottakis, Ioanna G. Maroulakou, Kevin Struhl, and Philip N. Tsichlis
     MicroRNAs Differentially Regulated by Akt Isoforms Control EMT and Stem Cell Renewal in Cancer Cells
     Sci. Signal., Oct 2009; 2: ra62.
    ... ...Human breast cancer samples RNAs from eight primary tumors and their corresponding metastatic tumors were purchased from Biochain Inc. These samples were used for real-time RT-PCR for E-cadherin, miR-200a, miR-200b, Akt1, and Akt2. Glyceraldehyde-3-phosphate... ...
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  • Sivan Osenberg, Dan Dominissini, Gideon Rechavi, and Eli Eisenberg
     Widespread cleavage of A-to-I hyperediting substrates
     RNA, Sep 2009; 15: 1632 - 1639.
    ... ...Experimental materials and methods Human adult hippocampus total RNA and genomic DNA from the same subject were purchased from Biochain. For RT-PCR, each RNA sample was treated with DNase I (Invitrogen) and reverse transcribed using M-MLV RT and random hexamers... ...
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  • Masayuki Kuraoka, Dongmei Liao, Kaiyong Yang, Sallie D. Allgood, Marc C. Levesque, Garnett Kelsoe, and Yoshihiro Ueda
     Activation-Induced Cytidine Deaminase Expression and Activity in the Absence of Germinal Centers: Insights into Hyper-IgM Syndrome
     J. Immunol., Sep 2009; 183: 3237 - 3248.
    .... ...populations, subcloned Ramos cells, and AMuLV transformants. Total RNA libraries of human adult tonsil (Biochain) and fetal liver (Biochain/Stratagene) were purchased; cDNA from mouse and human tissues was prepared by standard methods (12, 50... ...
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  • Celia M. Santi, Alice Butler, Julia Kuhn, Aguan Wei, and Lawrence Salkoff
     Bovine and Mouse SLO3 K+ Channels: EVOLUTIONARY DIVERGENCE POINTS TO AN RCK1 REGION OF CRITICAL FUNCTION
     J. Biol. Chem., Aug 2009; 284: 21589 - 21598.
    ... ...MATERIALS AND METHODS Cloning bSLO3 was cloned by reverse transcription-PCR of bovine testes total RNA (obtained from Biochain). First strand cDNA was made using Powerscript reverse transcriptase (Clontech) and random hexamers. Specific primers were... ...
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  • David Monk, Philippe Arnaud, Jennifer Frost, Frank A. Hills, Philip Stanier, Robert Feil, and Gudrun E. Moore
     Reciprocal imprinting of human GRB10 in placental trophoblast and brain: evolutionary conservation of reversed allelic expression
     Hum. Mol. Genet., Aug 2009; 18: 3066 - 3074.
    ... ...of the GRB10 isoforms arising from each promoter, we used custom-made fetal northern blots containing 2 microg poly A+ RNA (Biochain). PCR products containing the first exon associated with each promoter region (UN1-D86962; UN1A-NM_001001555; UN2-AJ271366... ...
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  • Yukimasa Takeda, Xiaohui Liu, Miho Sumiyoshi, Ayami Matsushima, Miki Shimohigashi, and Yasuyuki Shimohigashi
     Placenta Expressing the Greatest Quantity of Bisphenol A Receptor ERR{gamma} among the Human Reproductive Tissues: Predominant Expression of Type-1 ERR{gamma} Isoform
     J. Biochem., Jul 2009; 146: 113 - 122.
    ... ...prostate and testis) were purchased from Clontech, Stratagene and Biochain (Hayward, CA, USA). Each total RNA sample (1 mug) was reverse-transcribed...tissues were purchased from Clontech (C), Stratagene (S) and BioChain (B). b M and F represent male and female, respectively... ...
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  • Jin Billy Li, Erez Y. Levanon, Jung-Ki Yoon, John Aach, Bin Xie, Emily LeProust, Kun Zhang, Yuan Gao, and George M. Church
     Genome-Wide Identification of Human RNA Editing Sites by Parallel DNA Capturing and Sequencing
     Science, May 2009; 324: 1210 - 1213.
    ... ...References 30 We thank R. Emeson, M. P. Ball, and F. Isaacs for critical reading of the manuscript; P. Wang and Z. Liu (BioChain Institute) for helping collect human samples; M. Higuchi and P. Seeburg for providing ADAR2 / mouse brain cDNA; Harvard Biopolymers... ...
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  • Dimitrios Iliopoulos, Eirini I. Bimpaki, Maria Nesterova, and Constantine A. Stratakis
     MicroRNA Signature of Primary Pigmented Nodular Adrenocortical Disease: Clinical Correlations and Regulation of Wnt Signaling
     Cancer Res., Apr 2009; 69: 3278 - 3282.
    ... ...Four normal adrenal samples were used as controls; three of them were commercially available RNA adrenal samples (Ambion, Biochain) and a single normal adrenal tissue sample obtained from a cadaver through the National Development and Research Institutes... ...
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  • Wei Li, Dimitry M. Danilenko, Stuart Bunting, Rajkumar Ganesan, Susan Sa, Ronald Ferrando, Thomas D. Wu, Ganesh A. Kolumam, Wenjun Ouyang, and Daniel Kirchhofer
     The Serine Protease Marapsin Is Expressed in Stratified Squamous Epithelia and Is Up-regulated in the Hyperproliferative Epidermis of Psoriasis and Regenerating Wounds
     J. Biol. Chem., Jan 2009; 284: 218 - 228.
    ... ...system. EXPERIMENTAL PROCEDURES Reagents-Total RNA from human pancreas was purchased from different suppliers (Clontech, BioChain, Stratagene, Ambion, U. S. Biologicals, and Chemicon). Human and mouse total RNA adult tissue panels were purchased from Clontech... ...
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  • Willemijn M. Gommans, Nicholas E. Tatalias, Christina P. Sie, Dylan Dupuis, Nicholas Vendetti, Lauren Smith, Rikhi Kaushal, and Stefan Maas
     Screening of human SNP database identifies recoding sites of A-to-I RNA editing
     RNA, Oct 2008; 14: 2074 - 2085.
    ... ...RNA editing analysis For experimental validation, human brain total RNA and gDNA isolated from the same specimen (Biochain) were used and processed using standard protocols for reverse transcription and PCR (see Supplemental Table S1 for primer sequences... ...
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  • Atsushi Yonezawa, Satohiro Masuda, Toshiya Katsura, and Ken-ichi Inui
     Identification and functional characterization of a novel human and rat riboflavin transporter, RFT1
     Am J Physiol Cell Physiol, Sep 2008; 295: C632 - C641.
    ... ...thymus, small intestine, stomach, and liver) obtained from BioChain (Hayward, CA), rat tissues (brain, heart, lung, liver...intestine, spleen, kidney cortex, and kidney medulla) from BioChain, and cells (HEK-293 cells and Caco-2 cells) were reverse... ...
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  • Barbara Rosati, Min Dong, Lan Cheng, Shian-Ren Liou, Qinghong Yan, Ji Young Park, Elaine Shiang, Michael Sanguinetti, Hong-Sheng Wang, and David McKinnon
     Evolution of Ventricular Myocyte Electrophysiology
     Physiol Genomics, Sep 2008; 10.1152/physiolgenomics.00159.2007.
    ... ...was prepared using Qiagen RNeasy columns. Human RNA samples were obtained from independent commercial suppliers (Ambion or BioChain). Complementary DNAs were prepared as previously described (32). Three independent primer pairs for each gene were used for... ...
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  • Yoganand Balagurunathan, David L. Morse, Galen Hostetter, Vijayalakshmi Shanmugam, Phillip Stafford, Sonsoles Shack, John Pearson, Maria Trissal, Michael J. Demeure, Daniel D. Von Hoff, Victor J. Hruby, Robert J. Gillies, and Haiyong Han
     Gene expression profiling-based identification of cell-surface targets for developing multimeric ligands in pancreatic cancer
     Mol. Cancer Ther., Sep 2008; 7: 3071 - 3080.
    ... ...the Molecular Profiling Institute. RNA samples for normal tissues representing 28 different organ sites were purchased from Biochain and Stratagene. Paraffin-embedded pancreatic tissues were obtained from the Biospecimen Repository Core of the Pancreatic Cancer... ...
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  • Thomas Landemaine, Amanda Jackson, Akeila Bellahc¨¨ne, Nadia Rucci, Soraya Sin, Berta Martin Abad, Angels Sierra, Alain Boudinet, Jean-Marc Guinebreti¨¨re, Enrico Ricevuto, Catherine Nogu¨¨s, Marianne Briffod, Ivan Bi¨¨che, Pascal Cherel, Teresa Garcia, Vincent Castronovo, Anna Teti, Rosette Lidereau, and Keltouma Driouch
     A Six-Gene Signature Predicting Breast Cancer Lung Metastasis
     Cancer Res., Aug 2008; 68: 6092 - 6099.
    ... ...IDIBELL), respectively. RNA pools from normal lung and normal liver (at least five samples in each cases) were purchased from Biochain and Invitrogen. A series of 72 primary breast tumors (CRH cohort) was specifically selected from patients with node-negative... ...
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  • Tao Zhang, Cathie D. Xiang, David Gale, Samantha Carreiro, Ellen Y. Wu, and Eric Y. Zhang
     Drug Transporter and Cytochrome P450 mRNA Expression in Human Ocular Barriers: Implications for Ocular Drug Disposition
     Am J Physiol Renal Physiol, Jul 2008; 295: F165 - F170.
    ... ...consent. Quantification of OCT1, OCT2, and USF1 mRNA expression. Total RNA from various human tissues was purchased from BioChain (Hayward, CA). Reverse transcription of the total RNA (500 ng/20 ul reaction) and real-time PCR were carried out as described... ...
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  • Masayo Aoki, Tomohiro Terada, Moto Kajiwara, Ken Ogasawara, Iwao Ikai, Osamu Ogawa, Toshiya Katsura, and Ken-ichi Inui
     Kidney-specific expression of human organic cation transporter 2 (OCT2/SLC22A2) is regulated by DNA methylation
     Am J Physiol Renal Physiol, Jul 2008; 295: F165 - F170.
    ... ...consent. Quantification of OCT1, OCT2, and USF1 mRNA expression. Total RNA from various human tissues was purchased from BioChain (Hayward, CA). Reverse transcription of the total RNA (500 ng/20 ul reaction) and real-time PCR were carried out as described... ...
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  • Elizabeth Y. Rula, Andre H. Lagrange, Michelle M. Jacobs, NingNing Hu, Robert L. Macdonald, and Ronald B. Emeson
     Developmental Modulation of GABAA Receptor Function by RNA Editing
     J. Neurosci., Jun 2008; 28: 6196 - 6201.
    ... ...RNA (Sprague Dawley) was isolated as previously described (Burns et al., 1997), and human whole-brain RNA was purchased from BioChain Institute. As described in supplemental Methods (available at www.jneurosci.org as supplemental material ), first-strand... ...
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  • Amara C. Siva, Martha A. Wild, Richard E. Kirkland, Mary Jean Nolan, Bing Lin, Toshiaki Maruyama, Ferda Yantiri-Wernimont, Shana Frederickson, Katherine S. Bowdish, and Hong Xin
     Targeting CUB Domain-Containing Protein 1 with a Monoclonal Antibody Inhibits Metastasis in a Prostate Cancer Model
     Cancer Res., May 2008; 68: 3759 - 3766.
    ... ...pyrimidine] was purchased from Calbiochem. RNA samples. Total RNA for the normal tissue panel was purchased from BD Biosciences and BioChain Institute, Inc. Total RNA from frozen sections of prostate cell lines and severe combined immunodeficient (SCID) xenografts... ...
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  • Christopher Wright, Donald Bergstrom, Hongyue Dai, Matthew Marton, Mark Morris, George Tokiwa, Yanqun Wang, and Thomas Fare
    Characterization of Globin RNA Interference in Gene Expression Profiling of Whole-Blood Samples
    Clin. Chem., Feb 2008; 54: 396 - 405.
    ... ...the abundance of the b-globin message. supplementation with human brain total rna We obtained human brain total RNA from BioChain (catalog no. R1234035-50, lot no. A703158) and added it to aliquots of peripheral blood total RNA at the following concentrations... ...
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  • Germán Gastón Leparc and Robi David Mitra
    A sensitive procedure to detect alternatively spliced mRNA in pooled-tissue samples
    Nucleic Acids Res., Dec 2007; 35: e146.
    ... ...11-day, 15-day and 17-day mouse embryo, were obtained from Biochain (Cat No. R4334566, R1334XI-10, R1334XV-10 and R1334XVII-10...pancreas, uterus and prostate total RNA samples were obtained from BioChain (Cat No. R1234218-50, R1234086-50, R1234086-50, R1234188-50... ...
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  • Hironobu Sato, Hiromu Suzuki, Minoru Toyota, Masanori Nojima, Reo Maruyama, Shigeru Sasaki, Hideyasu Takagi, Yohei Sogabe, Yasushi Sasaki, Masashi Idogawa, Tomoko Sonoda, Mitsuru Mori, Kohzoh Imai, Takashi Tokino, and Yasuhisa Shinomura
    Frequent epigenetic inactivation of DICKKOPF family genes in human gastrointestinal tumors
    Carcinogenesis, Dec 2007; 28: 2459 - 2466.
    ... ...treated with a DNA-free kit (Ambion, Austin, TX). Total RNA from normal colon mucosa from a healthy individual was purchased from BioChain (Hayward, CA). Reverse transcriptase-polymerase chain reaction Single-stranded cDNA was prepared using SuperScript III... ...
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  • Hirohide Yoshikawa, Kenichi Matsubara, Xiaoling Zhou, Shu Okamura, Takahiko Kubo, Yaeko Murase, Yuko Shikauchi, Manel Esteller, James G. Herman, Xin Wei Wang, and Curtis C. Harris
    WNT10B Functional Dualism: -Catenin/Tcf-dependent Growth Promotion or Independent Suppression with Deregulated Expression in Cancer
    Mol. Biol. Cell, Nov 2007; 18: 4292 - 4303.
    ... ...medium, supplemented with 10% fetal bovine serum. Normal DNA and RNA samples (liver, colon, and placenta) were purchased from Biochain Institute (Hayward, CA) and BD Biosciences (Palo Alto, CA). Primary HCC samples are as described previously (Yoshikawa et al... ...
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  • Nurit Paz, Erez Y. Levanon, Ninette Amariglio, Amy B. Heimberger, Zvi Ram, Shlomi Constantini, Zohar S. Barbash, Konstantin Adamsky, Michal Safran, Avi Hirschberg, Meir Krupsky, Issachar Ben-Dov, Simona Cazacu, Tom Mikkelsen, Chaya Brodie, Eli Eisenberg, and Gideon Rechavi
    Altered adenosine-to-inosine RNA editing in human cancer
    Genome Res., Nov 2007; 17: 1586 - 1595.
    ... ...Center, Tel Aviv Sourasky Medical Center, or Henry Ford Hospital (Detroit, MI), or were purchased (Stratagene, Clontech, and Biochain). Malignant and normal samples from oral cavity (six subjects) or lung (five subjects) were collected at the Chaim Sheba Medical... ...
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  • Yakun Chen, Yong Tang, Man-Tzu Wang, Su Zeng, and Daotai Nie
    EXPERIMENTAL THERAPEUTICS, MOLECULAR TARGETS, AND CHEMICAL BIOLOGY: Human Pregnane X Receptor and Resistance to Chemotherapy in Prostate Cancer
    Cancer Res., Nov 2007; 67: 10361 - 10367.
    ... ...of PXR or its target genes, CYP3A4 and MDR1, was evaluated by semiquantitative RT-PCR. A normal human colon tissue sample (BioChain Institute, Inc.) and a human liver cancer cell line, HepG2, were used in parallel to compare the transcript levels of PXR in... ...
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  • Emiko Hayashi, Yuriko Matsuzaki, Go Hasegawa, Tomonori Yaguchi, Sachiko Kurihara, Tomonobu Fujita, Toshiro Kageshita, Makoto Sano, and Yutaka Kawakami
    Identification of a Novel Cancer-Testis Antigen CRT2 Frequently Expressed in Various Cancers Using Representational Differential Analysis
    Clin. Cancer Res., Nov 2007; 13: 6267 - 6274.
    ... ...fetal brain, fetal thymus, thymus, and fetal liver were purchased from Clontech. Total RNA from esophagus was purchased from Biochain Institute, Inc. The malignant melanoma, esophageal cancer, gastric cancer, pancreatic ductal adenocarcinoma, colon cancer... ...
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  • Changming Fang, Jarrod Dean, and Jeffrey W. Smith
    A Novel Variant of Ileal Bile Acid Binding Protein Is Up-regulated through Nuclear Factor-B Activation in Colorectal Adenocarcinoma
    Cancer Res., Oct 2007; 67: 9039 - 9046.
    ... ...quantitative reverse transcription-PCR (RT-PCR) with RNA from normal human intestine and liver purchased from Invitrogen and from BioChain Institute, Inc. Expression of acidic ribosomal phosphoprotein P0 (ARPP0) was used as a control. The expression of IBABP-L... ...
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  • Osamu Seguchi, Seiji Takashima, Satoru Yamazaki, Masanori Asakura, Yoshihiro Asano, Yasunori Shintani, Masakatsu Wakeno, Tetsuo Minamino, Hiroya Kondo, Hidehiko Furukawa, Kenji Nakamaru, Asuka Naito, Tomoko Takahashi, Toshiaki Ohtsuka, Koichi Kawakami, Tadashi Isomura, Soichiro Kitamura, Hitonobu Tomoike, Naoki Mochizuki, and Masafumi Kitakaze
    A cardiac myosin light chain kinase regulates sarcomere assembly in the vertebrate heart
    J. Clin. Invest., Oct 2007; 117: 2812 - 2824.
    ... ...and ejection fraction (EF) was measured by echocardiography the day before the operation. Normal samples were purchased from Biochain Inc. Cardiac gene expression was determined using the HG-U95 Affymetrix GeneChip. All expression data were normalized by global... ...
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  • Noa Matarasso, Anat Bar-Shira, Uri Rozovski, Serena Rosner, and Avi Orr-Urtreger
    Functional Analysis of the Aurora Kinase A Ile31 Allelic Variant in Human Prostate
    Neoplasia. 2007 September; 9(9): 707?15.
    ... ...Twenty-eight RNA samples from donor prostate tissues classified as normal prostate were purchased: 27 samples from donors of Asian descent (BioChain Institute, Inc., Hayward, CA) and 1 sample from a donor of Caucasian descent (Ambion, Inc., Austin, TX).... ...
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  • Yi-Mei J. Lin, Shin-Chih Chao, Tsung-Ming Chen, Te-Jen Lai, Jia-Shing Chen, and H. Sunny Sun
    Association of Functional Polymorphisms of the Human Tryptophan Hydroxylase 2 Gene With Risk for Bipolar Disorder in Han Chinese
    Arch Gen Psychiatry, Sep 2007; 64: 1015 - 1024.
    ... ...on our Web site), respectively. Using Q-RT-PCR, 8 complementary DNA libraries made from various sections of the human brain (BioChain Institute, Hayward, California) were used to assay the relative messenger RNA (mRNA) expression level of the TPH1 and TPH2... ...
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  • Hirohide Yoshikawa, Kenichi Matsubara, Xiaoling Zhou, Shu Okamura, Takahiko Kubo, Yaeko Murase, Yuko Shikauchi, Manel Esteller, James G. Herman, Xin Wei Wang, and Curtis C. Harris
    WNT10B Functional Dualism: -Catenin/Tcf-dependent Growth Promotion or Independent Suppression with Deregulated Expression in Cancer
    Mol. Biol. Cell, Aug 2007; 10.1091/mbc.E06-10-0889.
    ... ...1640, supplemented with 10% fetal bovine serum. Normal DNA and RNA samples (liver, colon, and placenta) were purchased from Biochain Institute, Inc. (Hayward, CA) and BD Biosciences (Palo Alto, CA). Primary HCC samples are as described(Ye et al., 2003; Yoshikawa... ...
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  • Damian G Romero, Ming Yi Zhou, Licy L Yanes, Maria W Plonczynski, Tanganika R Washington, Celso E Gomez-Sanchez, and Elise P Gomez-Sanchez
    Regulators of G-protein signaling 4 in adrenal gland: localization, regulation, and role in aldosterone secretion
    J. Endocrinol., Aug 2007; 194: 429 - 440.
    ... ...generously provided Losartan. Human adrenal total RNA was obtained from several sources: hAd1 (59-year-old male donor) from BioChain Institute Inc. (Hayward, CA, USA), hAd2 (pooled from 61 male/female Caucasian donors, ages 15-61) from BD Biosciences (Mountain... ...
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  • Junichi Enokizono, Hiroyuki Kusuhara, and Yuichi Sugiyama
    Role of Breast Cancer Resistance Protein (Bcrp/Abcg2) in the Disposition of Phytoestrogens : the Importance of Bcrp in the Efflux Transport in the Blood-Brain and -Testis Barriers
    Mol. Pharmacol., Jul 2007; 10.1124/mol.107.034751.
    ... ...isolated from these tissues with using ISOGEN (Wako pure chemical industries). Total RNA of human epididymis was purchased from BioChain Institute (Hayward, CA). Total RNA from mouse and human epididymis was converted to cDNA using random primer and avian myeloblastosis... ...
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  • Debopriya Das, Tyson A. Clark, Anthony Schweitzer, Miki Yamamoto, Henry Marr, Josh Arribere, Simon Minovitsky, Alexander Poliakov, Inna Dubchak, John E. Blume, and John G. Conboy
    A correlation with exon expression approach to identify cis-regulatory elements for tissue-specific alternative splicing
    Nucleic Acids Res., Jul 2007; 35: 4845 - 4857.
    ... ...Total RNA from three biological replicates (three separate individuals) of 16 normal adult human tissues was purchased from BioChain (Hayward, CA, USA). Labeled target was generated from 200ng of total RNA and hybridized to a prototype version of the Affymetrix... ...
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  • Ayush Dagvadorj, Sean Collins, Jean-Baptiste Jomain, Junaid Abdulghani, James Karras, Tobias Zellweger, Hongzhen Li, Martti Nurmi, Kalle Alanen, Tuomas Mirtti, Tapio Visakorpi, Lukas Bubendorf, Vincent Goffin, and Marja T. Nevalainen
    Autocrine Prolactin Promotes Prostate Cancer Cell Growth via Janus Kinase-2-Signal Transducer and Activator of Transcription-5a/b Signaling Pathway
    Endocrinology, Jul 2007; 148: 3089 - 3101.
    ... ...Pit1). The size of the PCR product yielded by this primer pair was 440 bp. Human brain pituitary total RNA was purchased from BioChain Institute, Inc. (Hayward, CA) and was used as positive control. Luciferase reporter gene assay CWR22Rv cells were transfected... ...
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  • Evemie Dub? Peter T.K. Chan, Louis Hermo, and Daniel G. Cyr
    Gene Expression Profiling and Its Relevance to the Blood-Epididymal Barrier in the Human Epididymis
    Biol Reprod, Jun 2007; 76: 1034 - 1044.
    ... ...presence of transcripts for the different CLDN genes. Total RNA (500 ng) was obtained from two different individuals using Biochain (Hayward, CA) and reverse-transcribed using an oligo(dT)16 primer. Forward and reverse primers for the genes of interest were... ...
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  • Glenn S. Belinsky, Ann L. Parke, Qihong Huang, Kerry Blanchard, Supriya Jayadev, Raymond Stoll, Marti Rothe, Luke E. K. Achenie, Rishi R. Gupta, George Y. Wu, and Daniel W. Rosenberg
    The Contribution of Methotrexate Exposure and Host Factors on Transcriptional Variance in Human Liver
    Toxicol. Sci., Jun 2007; 97: 582 - 594.
    ... ...pathologists at JDH. Total RNA from normal human livers was obtained from Ambion (Austin, TX), Clontech (Mountain View, CA), and Biochain (Hayward, CA). The normal group of livers consisted of one female and nine males ranging in age from 24 to 70 years. Pathologic... ...
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  • Ayush Dagvadorj, Sean Collins, Jean-Baptiste Jomain, Junaid Abdulghani, James Karras, Tobias Zellweger, Hongzhen Li, Martti Nurmi, Kalle Alanen, Tuomas Mirtti, Tapio Visakorpi, Lukas Bubendorf, Vincent Goffin, and Marja T. Nevalainen
    Autocrine Prolactin Promotes Prostate Cancer Cell Growth via Jak2-Stat5a/b Signaling Pathway
    Endocrinology, Apr 2007; 10.1210/en.2006-1761.
    ... ...000306). The size of the PCR product yielded by this primer pair is 440 bp. Human brain pituitary total RNA was purchased from BioChain Institute, Inc. (Hayward, CA) and was used as positive control. Luciferase Reporter Gene Assay CWR22Rv cells were transfected... ...
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  • Evemie Dub? Peter T.K. Chan, Louis Hermo, and Daniel G. Cyr
    Gene Expression Profiling and Its Relevance to the Blood-Epididymal Barrier in the Human Epididymis
    Biol Reprod, Feb 2007; 10.1095/biolreprod.106.059246.
    ... ...the presence of transcripts for the different CLDN genes. Total RNA (500 ng) was obtained from two different individuals from Biochain (Hayward, CA, USA) and was reverse transcribed using an Oligo d(T)16 primer. Forward and reverse primers for the genes of interest... ...
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  • E. Kostova, C.H. Yeung, C.M. Luetjens, M. Brune, E. Nieschlag, and J. Gromoll
    Association of three isoforms of the meiotic BOULE gene with spermatogenic failure in infertile men
    Mol. Hum. Reprod., Feb 2007; 13: 85 - 93.
    ... ...diagnosis in the case of MA and SCO. RNA extraction, RT and quantitative PCR Commercially derived testis total RNA (BioChain, Hayward, CA, USA) from two healthy men was used as control to analyse the normal distribution of BOULE isoforms. Total RNA... ...
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  • Durocher F, Labrie Y, Soucy P, Sinilnikova O, Labuda D, Bessette P, Chiquette J, Laframboise R, Lépine J, Lespérance B, Ouellette G, Pichette R, Plante M, Tavtigian SV, Simard J.
    Mutation analysis and characterization of ATR sequence variants in breast cancer cases from high-risk French Canadian breast/ovarian cancer families
    BMC Cancer. 2006; 6: 230. published online before print September 29, 2006
    ... ...Total RNA samples from normal tissues were purchased directly either from Stratagene (breast and ovary) (La Jolla, CA, USA), BioChain Institute Inc. (leukocyte) (Hayward, CA, USA),... ...
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  • Damian G. Romero, Maria W. Plonczynski, Elise P. Gomez-Sanchez, Licy L. Yanes, and Celso E. Gomez-Sanchez
    RGS2 Is Regulated by Angiotensin II and Functions as a Negative Feedback of Aldosterone Production in H295R Human Adrenocortical Cells
    Endocrinology, Aug 2006; 147: 3889 - 3897.
    ... ...from Tocris (Ellisville, MO). Human adrenal total RNA was obtained from several sources: hAd1 (59-yr-old male donor) from BioChain Institute, Inc. (Hayward, CA)... ...
  • Tae-You Kim, Sheng Zhong, C. Robert Fields, Jeong Hoon Kim, and Keith D. Robertson
    Epigenomic Profiling Reveals Novel and Frequent Targets of Aberrant DNA Methylation-Mediated Silencing in Malignant Glioma
    Cancer Res., Aug 2006; 66: 7490 - 7501.
    ... ...chloroform extraction method (17). Two normal brain DNA and RNA samples from cancer-free individuals were purchased from BioChain (Hayward, CA)... ...
  • Satohiro Masuda, Tomohiro Terada, Atsushi Yonezawa, Yuko Tanihara, Koshiro Kishimoto, Toshiya Katsura, Osamu Ogawa, and Ken-ichi Inui
    Identification and Functional Characterization of a New Human Kidney–Specific H+/Organic Cation Antiporter, Kidney-Specific Multidrug and Toxin Extrusion 2
    J. Am. Soc. Nephrol., Aug 2006; 17: 2127 - 2135
    ... ...AB250364 and AB250701 , respectively. Real-Time PCR and RT-PCR Total RNA from various human tissues was purchased from BioChain (Hayward, CA). RT of the total RNA (500 ng/20 Ml reaction) and real-time PCR were carried out as described previously... ...

  • Vincent Castronovo, David Waltregny, Philippe Kischel, Christoph Roesli, Giuliano Elia, Jascha N. Rybak, and Dario Neri
    A chemical proteomic approach for the identification of accessible antigens expressed in human kidney cancer
    Mol. Cell. Proteomics, Jul 2006; 10.1074/mcp.M600164-MCP200.
    ... ...cell carcinoma, granular cell carcinoma, transitional cell carcinoma, normal adult and fetal kidney were purchased from BioChain (Hayward, CA, USA)... ...

  • Jae-Hyun Park, Meng-Lay Lin, Toshihiko Nishidate, Yusuke Nakamura, and Toyomasa Katagiri
    PDZ-Binding Kinase/T-LAK Cell-Originated Protein Kinase, a Putative Cancer/Testis Antigen with an Oncogenic Activity in Breast Cancer
    Cancer Res., Sep 2006; 66: 9186 - 9195.
    ... ...of mRNA isolated from normal adult human mammary gland (Biochain, Hayward, CA), lung, heart, liver, kidney, and bone marrow...tissue sections of heart, lung, liver, kidney, and testis (Biochain). Briefly, paraffin-embedded specimens were treated with... ...
    Ref. link

  • Døsen G, Tenstad E, Nygren MK, Stubberud H, Funderud S, Rian E.
    Wnt expression and canonical Wnt signaling in human bone marrow B lymphopoiesis
    BMC Immunol. 2006; 7: 13. published online before print June 29, 2006
    ... ...Total RNA from freshly isolated and sorted BM CD45+CD10+IgM- cells was isolated using Absolutely RNA?RT-PCR Mini-prep kit (Stratagene Europe, Amsterdam, Netherland) according to the manufacturer's instructions. RNA from human fetal brain was purchased from BioChain Institute, Inc., USA. ... ...
    Ref. link

  • Blow MJ, Grocock RJ, van Dongen S, Enright AJ, Dicks E, Futreal PA, Wooster R, Stratton MR.
    RNA editing of human microRNAs
    Genome Biol. 2006; 7(4): R27.
    ... ...For the initial screen of RNA editing in ten human tissues, total RNA and matching genomic DNA from the same tissue sample was obtained for human brain, heart, liver, lung, ovary, placenta, skeletal muscle, small intestine, spleen and testis from Biochain (Hayward, USA). ... ...
    Ref. link

  • Goldstein M, Meller I, Issakov J, Orr-Urtreger A.
    Novel Genes Implicated in Embryonal, Alveolar, and Pleomorphic Rhabdomyosarcoma: A Cytogenetic and Molecular Analysis of Primary Tumors
    Neoplasia. 2006 May; 8(5): 332-343.
    ... ...As normal controls, we purchased four different RNA samples that were extracted from normal adult skeletal muscles (Clontech BD Biosciences, Erembodegem, Belgium; Ambion, Inc., Austin, TX; BioChain Institute, Inc., Hayward, CA). ... ...
    Ref. link

  • Human Umbilical Cord Matrix Stem Cells: Preliminary Characterization and Effect of Transplantation in a Rodent Model of Parkinson’s Disease
    Mark L. Weiss, Satish Medicetty, Amber R. Bledsoe, Raja Shekar Rachakatla, Michael Choi, Shosh Merchav, Yongquan Luo, Mahendra S. Rao, Gopalrao Velagaleti, and Deryl Troyer
    Stem Cells, Mar 2006; 24: 781 - 792.
    ... ...positive human control RNA (liver, placenta, and muscle; BioChain, Hayward, CA, http://www.biochain.com ) was evaluated using RT-PCR to confirm or expand... ...

  • Wei Zhou, Zhilin Liu, Jianbo Wu, Jing-hua Liu, Salman M. Hyder, Eric Antoniou, and Dennis B. Lubahn
    Identification and Characterization of Two Novel Splicing Isoforms of Human Estrogen-Related Receptor ß
    J. Clin. Endocrinol. Metab., Feb 2006; 91: 569 - 579.
    ... ...A third placenta total RNA was purchased from BioChain Institute, Inc. (Hayward, CA). .. ...

  • Damian G. Romero, Gaston R. Vergara, Zheng Zhu, Gina S. Covington, Maria W. Plonczynski, Licy L. Yanes, Elise P. Gomez-Sanchez, and Celso E. Gomez-Sanchez
    Interleukin-8 Synthesis, Regulation, and Steroidogenic Role in H295R Human Adrenocortical Cells
    Endocrinology, Feb 2006; 147: 891 - 898.
    ... ...cortisol. Real time RT-PCR Human adrenal total RNA was obtained from several sources: hAd1 (59 yr old male donor) from BioChain Institute, Inc. (Hayward, CA),... ...

  • Helene Baribault, Jean Danao, Jamila Gupte, Li Yang, Banghua Sun, William Richards, and Hui Tian
    The G-Protein-Coupled Receptor GPR103 Regulates Bone Formation
    Mol. Cell. Biol., Jan 2006; 26: 709 - 717.
    ... ...visualized by autoradiography and light microscopy. Quantitative RT-PCR was performed on human total RNA from various tissues (BioChain or Clontech) using the Taqman Gold kit (Applied Biosystems) and primer and probe set 5-GGGCTTTCACAATGCTAG-3, 5-GGAAGTCATATTTGATCTC-3... ...
  • Stephen J. Elliman, Isaac Wu, and Daniel M. Kemp
    Adult Tissue-specific Expression of a Dppa3-derived Retrogene Represents a Postnatal Transcript of Pluripotent Cell Origin
    J. Biol. Chem., Jan 2006; 281: 16 - 19.
    ... ...integrity of each sample. Pancreas cDNA was obtained from BioChain, and RACE (rapid amplification of cDNA ends) cDNA was...thyroid gland, heart and adrenal gland were obtained from BioChain. Each reaction well contained 5 mul of forward primer... ...

  • Bekpen C, Hunn JP, Rohde C, Parvanova I, Guethlein L, Dunn DM, Glowalla E, Leptin M, Howard JC.
    The interferon-inducible p47 (IRG) GTPases in vertebrates: loss of the cell autonomous resistance mechanism in the human lineage
    Genome Biol. 2005; 6(11): R92.
    ... ...Poly(A) RNA was isolated from total RNA using the Oligotex mRNA kit (QIAGEN). Total RNA from human tissues was purchased from Biochain (Hayward, CA, USA).... ...
    Ref. link

  • Renate Parry, Doug Schneider, Debra Hudson, Debbie Parkes, Jian-Ai Xuan, Alicia Newton, Pam Toy, Rick Lin, Rick Harkins, Bruno Alicke, Sandra Biroc, Peter J. Kretschmer, Meredith Halks-Miller, Helmut Klocker, Ying Zhu, Brent Larsen, Ronald R. Cobb, Peter Bringmann, Georg Roth, Jason S. Lewis, Harald Dinter, and Gordon Parry
    Identification of a Novel Prostate Tumor Target, Mindin/RG-1, for Antibody-Based Radiotherapy of Prostate Cancer
    Cancer Res., Sep 2005; 65: 8397 - 8405.
    ... ...Tumor tissue total RNA was purchased from BioChain (Hayward, CA). A real-time quantitative reverse transcription-PCR assay was developed to measure mindin/RG-1 using specific... ...

  • John Backus, Todd Laughlin, Yixin Wang, Robert Belly, Robert White, Jon Baden, C. Justus Min, Ann Mannie, Lorraine Tafra, David Atkins, and Kathryn M. Verbanac
    Identification and Characterization of Optimal Gene Expression Markers for Detection of Breast Cancer Metastasis
    Mol. Diagn., Aug 2005; 7: 327 - 336.
    ... ...medullary). Twenty-four tissue RNA samples from lung, colon, rectum, and ovary (6 normal and 18 cancerous; procured from BioChain, Inc.) were used as tissue specificity samples, along with a white blood cell (WBC) total RNA pool derived from 24 female... ...

  • Tatiana Ort, Anibal A. Arjona, John R. MacDougall, Pam J. Nelson, Mark E. Rothenberg, Frank Wu, Andrew Eisen, and Yuan-Di C. Halvorsen
    Recombinant Human FIZZ3/Resistin Stimulates Lipolysis in Cultured Human Adipocytes, Mouse Adipose Explants, and Normal Mice
    Endocrinology, May 2005; 146: 2200 - 2209.
    ... ...real-time quantitative PCR (RTQ-PCR). Total RNA from various human tissues was acquired from Stratagene (La Jolla, CA) and BioChain Institute (Hayward, CA). All tissue samples were received from certified hospitals with informed consent from donors or... ...

  • Xiao-Yan Zhao, Doug Schneider, Sandra L. Biroc, Renate Parry, Bruno Alicke, Pamela Toy, Jian-Ai Xuan, Choitsu Sakamoto, Ken Wada, Michael Schulze, Beate Müller-Tiemann, Gordon Parry, and Harald Dinter
    Targeting Tomoregulin for Radioimmunotherapy of Prostate Cancer
    Cancer Res., Apr 2005; 65: 2846 - 2853.
    ... ...tumor tissues were purified from clinical samples, and normal human tissue RNA was purchased from a commercial vendor (BioChain, Hayward, CA). Solid bars, normal prostate (N. prostate), benign prostatic hyperplasia (BPH Prostate), and prostate tumor... ...

  • Thomas A. Hughes and Hugh J. M. Brady
    Expression of axin2 Is Regulated by the Alternative 5'-Untranslated Regions of Its mRNA
    J. Biol. Chem., Mar 2005; 280: 8581 - 8588.
    .... RNA and protein that had been purified simultaneously from single tissue samples was purchased from BioChain (AMS Biotechnology). cDNA samples were subjected to semi-quantitative PCR using RedTaq (Sigma) and the following ...

  • Guenther Boden, Carol Homko, Maria Mozzoli, Louise C. Showe, Calen Nichols, and Peter Cheung
    Thiazolidinediones Upregulate Fatty Acid Uptake and Oxidation in Adipose Tissue of Diabetic Patients
    Diabetes, Mar 2005; 54: 880 - 885.
    ... Control RNAs were human adult adipose tissue RNA and skeletal muscle RNA (purchased from BioChain, Haywood, CA). ...

  • Erez Y. Levanon, Martina Hallegger, Yaron Kinar, Ronen Shemesh, Kristina Djinovic-Carugo, Gideon Rechavi, Michael F. Jantsch, and Eli Eisenberg
    Evolutionarily conserved human targets of adenosine to inosine RNA editing
    Nucleic Acids Res., Feb 2005; 33: 1162 - 1168.
    ... RNA and genomic DNA (gDNA) isolated simultaneously from the same tissue sample were purchased from Biochain Institute (Hayward, CA). ...

  • Mingjun Liu, Yubin Ge, Diane C. Cabelof, Amro Aboukameel, Ahmad R. Heydari, Ramzi Mohammad, and Larry H. Matherly
    Structure and Regulation of the Murine Reduced Folate Carrier Gene: IDENTIFICATION OF FOUR NONCODING EXONS AND PROMOTERS AND REGULATION BY DIETARY FOLATES
    Biol. Chem., Feb 2005; 280: 5588 - 5597.
     ... Total RNA from mouse small intestine was purchased from BioChain Institute, Inc. ...

  • Tatiana Ort, Anibal A. Arjona, John R. MacDougall, Pam J. Nelson, Mark E. Rothenberg, Frank Wu, Andrew Eisen, and Yuan-Di C. Halvorsen
    Recombinant Human FIZZ3/Resistin Stimulates Lipolysis in Cultured Human Adipocytes, Mouse Adipose Explants and in Normal Mice
    Endocrinology, Feb 2005; 10.1210/en.2004-1421.
     ... Total RNA from various human tissues was acquired from Stratagene (La Jolla, CA) and BioChain Institute (Hayward, CA). ...

  • Vladimir P. Grinevich, Sharon R. Letchworth, Kari A. Lindenberger, Jean Menager, Veronique Mary, Khalima A. Sadieva, Lori M. Buhlman, Georg Andrees Bohme, Laurent Pradier, Jesus Benavides, Ronald J. Lukas, and Merouane Bencherif
    Heterologous Expression of Human {alpha}6{beta}4{beta}3{alpha}5 Nicotinic Acetylcholine Receptors: Binding Properties Consistent with Their Natural Expression Require Quaternary Subunit Assembly Including the {alpha}5 Subunit
    Pharmacol. Exp. Ther., Feb 2005; 312: 619 - 626.
     ... for total brain and cortex was obtained from BD Biosciences Clontech (Palo Alto, CA) and Biochain (Hayward, CA), respectively. ...

  • Omer Barad, Eti Meiri, Amir Avniel, Ranit Aharonov, Adi Barzilai, Isaac Bentwich, Uri Einav, Shlomit Gilad, Patrick Hurban, Yael Karov, Edward K. Lobenhofer, Eilon Sharon, Yoel M. Shiboleth, Marat Shtutman, Zvi Bentwich, and Paz Einat
    MicroRNA expression detected by oligonucleotide microarrays: System establishment and expression profiling in human tissues
    Genome Res., Dec 2004; 14: 2486 - 2494.
    ... brain RNA from Ambion, whereas Sempere et al. ( 2004 ) obtained it from the Biochain Institute. ...

  • Ken-ichiro Okada, Tetsuo Minamino, Yoshitane Tsukamoto, Yulin Liao, Osamu Tsukamoto, Seiji Takashima, Akio Hirata, Masashi Fujita, Yoko Nagamachi, Takeshi Nakatani, Chikao Yutani, Kentaro Ozawa, Satoshi Ogawa, Hitonobu Tomoike, Masatsugu Hori, and Masafumi Kitakaze
    Prolonged Endoplasmic Reticulum Stress in Hypertrophic and Failing Heart After Aortic Constriction: Possible Contribution of Endoplasmic Reticulum Stress to Cardiac Myocyte Apoptosis
     Circulation, Aug 2004; 110: 705 - 712.
     ... As the normal controls, we used total human heart RNA purchased from BD Bioscience and BioChain. ...

     ... Lanes 1 and 2 show normal human heart specimens obtained from Clontech and BioChain, respectively. ...

  • Begovich AB, Carlton VE, Honigberg LA, Schrodi SJ, Chokkalingam AP, Alexander HC, Ardlie KG, Huang Q, Smith AM, Spoerke JM, Conn MT, Chang M, Chang SY, Saiki RK, Catanese JJ, Leong DU, Garcia VE, McAllister LB, Jeffery DA, Lee AT, Batliwalla F, Remmers E, Criswell LA, Seldin MF, Kastner DL, Amos CI, Sninsky JJ, Gregersen PK.
    A Missense Single-Nucleotide Polymorphism in a Gene Encoding a Protein Tyrosine Phosphatase (PTPN22) Is Associated with Rheumatoid Arthritis
    Am J Hum Genet. 2004 Aug; 75(2): 330-337.
    ... ...Tissues: Adipose BioChain -2.21 ... ...
    ... ...Tissues: Esophagus BioChain -3.61 ... ...
    ... ...Tissues: Breast BioChain -1.98 ... ...
    ... ...Tissues: Heart (pericardium) BioChain -1.56 ... ...
    ... ...Tissues: Pancreas BioChain -3.34 ... ...
    ... ...Tissues: Stomach BioChain -2.16 ... ...
    ... ...Tissues: Umbilical cord (fetal) BioChain -1.37 ... ...
    Ref. link

  • Olsen CL, Hsu PP, Glienke J, Rubanyi GM, Brooks AR.
    Hedgehog-interacting protein is highly expressed in endothelial cells but down-regulated during angiogenesis and in several human tumors
    BMC Cancer. 2004; 4: 43. published online before print August 4, 2004
    ... ...we measured HIP mRNA levels in a variety of tumor samples. Tumor and corresponding normal tissue RNA samples (normal and tumor tissues were from different individuals) were obtained from two different commercial sources (Ambion and BioChain), and the endothelial cell marker vWF was used to normalize HIP expression to the number of endothelial cells present in the tumors. ... ...
    Ref. link

  • Rotem Sorek, Ronen Shemesh, Yuval Cohen, Ortal Basechess, Gil Ast, and Ron Shamir
    A Non-EST-Based Method for Exon-Skipping Prediction
    Genome Res., Aug 2004; 14: 1617 - 1623.
    ... from the following human tissue samples: (1) Brain pool, a pool of brain-derived RNA samples (Biochain ?Normal); (2) Prostate pool, a pool of prostate-derived RNA samples (Biochain ?Normal); (3) ...
    ... Testis pool, a pool of testis-derived RNA samples (Biochain ?Normal); (4) Kidney pool, a pool of kidney-derived RNA samples (Biochain ? Normal); (5) ...

  • Sherif F. Nagueh, Gopi Shah, Yiming Wu, Guillermo Torre-Amione, Nicholas M.P. King, Sunshine Lahmers, Christian C. Witt, Katy Becker, Siegfried Labeit, and Henk L. Granzier
    Altered Titin Expression, Myocardial Stiffness, and Left Ventricular Function in Patients With Dilated Cardiomyopathy
     Circulation, Jul 2004; 110: 155 - 162.
     ... myocardium (obtained from 6 males, aged 21 to 74 years, mean 37+-9 years; Ambion and Biochain Institute Inc). ...

  • Hideto Jinno, Toshiko Tanaka-Kagawa, Nobumitsu Hanioka, Seiich Ishida, Mayumi Saeki, Akiko Soyama, Masaya Itoda, Tetsuji Nishimura, Yoshiro Saito, Shogo Ozawa, Masanori Ando, and Jun-ichi Sawada
    Identification of Novel Alternative Splice Variants of Human Constitutive Androstane Receptor and Characterization of Their Expression in the Liver
    Mol. Pharmacol., Mar 2004; 65: 496 - 502.
    ... Human liver total RNA (of male subjects aged 24?4) was obtained from Biochain (Hayward, CA). ... 

  • Sempere LF, Freemantle S, Pitha-Rowe I, Moss E, Dmitrovsky E, Ambros V.
    Expression profiling of mammalian microRNAs uncovers a subset of brain-expressed microRNAs with possible roles in murine and human neuronal differentiation
    Genome Biol. 2004; 5(3): R13. published online before print February 16, 2004
    ... ...Frozen dissected organs from six-week-old C57BL/6 mice were a gift from Nathan Watson (Norris Cotton Cancer Center at Dartmouth-Hitchcock Medical Center). Total RNA was extracted from pooled organs of two to four mice or from cell lines with Trizol Reagent following the vendor's recommendations. Total RNA, 5 or 10 µg, was routinely loaded onto 12% urea-polyacrylamide gels as described in [50]. Total RNA from human organs was acquired from the Biochain Institute. Membranes were stripped by boiling in 0.1% SDS and re-hybridized up to five times without observing decreases in quality ... ...
    Ref. link

  • Winkler DG, Sutherland MK, Geoghegan JC, Yu C, Hayes T, Skonier JE, Shpektor D, Jonas M, Kovacevich BR, Staehling-Hampton K, Appleby M, Brunkow ME, Latham JA.
    Osteocyte control of bone formation via sclerostin, a novel BMP antagonist
    EMBO J. 2003 Dec 1; 22(23): 6267-6276.
    ... ...hMSCs cultured in growth (undifferentiated hMSCs) or osteogenic (hMSCs to osteoblasts) medium were harvested 21 days after plating and RNA isolated for RT–PCR analyses of SOST, COL1A1, PPAR?2, PTHR1, COLIXA1 and COLXA1. SOST expression was also determined in RNA prepared from abdominal adipose tissue (Biochain Institute, Hayward, CA), cartilage tissue from femoral growth plates (Biochain Institute), primary cultures of human osteoblasts . ... ...
    Ref. link

  • Bijay S. Jaiswal and Marco Conti
    Calcium regulation of the soluble adenylyl cyclase expressed in mammalian spermatozoa
     PNAS, Sep 2003; 100: 10676 - 10681.
    ... Human total testis RNA was obtained from Clontech and Biochain Institute (Hayward, CA). ...

  • Dirk Usener, Dirk Schadendorf, Joachim Koch, Stefan Dübel, and Stefan Eichmüller
    cTAGE: A Cutaneous T Cell Lymphoma Associated Antigen Family with Tumor-Specific Splicing
     J. Invest. Dermatol., Jul 2003; 121: 198 - 206.
    ... tissues: bone marrow, brain, colon, placenta, prostate, spleen, skeletal muscle, small intestine, trachea, stomach, testis; BioChain Institute, Hayward, CA, and Becton Dickinson Clontech). ...

  • Noriko Okazaki, Naomi Takahashi, Shin-ichi Kojima, Yasuhiko Masuho, and Hisashi Koga
    Protocadherin LKC, a new candidate for a tumor suppressor of colon and liver cancers, its association with contact inhibition of cell proliferation
    Carcinogenesis, Jul 2002; 23: 1139 - 1148. 
    ... The total RNA-Human Normal Colon was purchased from BioChain Institute (Hayward, CA). ...

  • David R. Dorris, Ramesh Ramakrishnan, Dionisios Trakas, Frank Dudzik, Richard Belval, Connie Zhao, Allen Nguyen, Marc Domanus, and Abhijit Mazumder
    A Highly Reproducible, Linear, and Automated Sample Preparation Method for DNA Microarrays
    Genome Res., Jun 2002; 12: 976 - 984. 
    ... Preparation Total RNA was purchased for all tissues and the Burkitt's lymphoma cell lines (Ambion; BioChain; Clontech), or prepared from the hepatocarcinoma cell line HepG2 using the TriZOL method (Invitrogen). ...

  • Zhi-Bin Tong, Carolyn A. Bondy, Jian Zhou, and Lawrence M. Nelson
    A human homologue of mouse Mater, a maternal effect gene essential for early embryonic development
    Hum. Reprod., Apr 2002; 17: 903 - 911. 
    ... Ovarian RNA from a 26 year old woman was purchased from BioChain Inc. ...

  • Jian Jin, Xuehui Chen, Yan Zhou, Mark Bartlam, Qing Guo, Yiwei Liu, Yixin Sun, Yu Gao, Sheng Ye, Guangtao Li, Zihe Rao, Boqin Qiang, and Jiangang Yuan
    Crystal structure of the catalytic domain of a human thioredoxin-like protein: Implications for substrate specificity and a novel regulation mechanism
    Eur. J. Biochem., Apr 2002; 269: 2060 - 2068.
    ... DDRT-PCR and full-length cDNA isolation Total RNA (2.5 µg) from 13- and 33-week-old human fetal cerebrum (Biochain) was reverse transcribed by Superscript II (Gibco-BRL) using a single-base anchored 3′ primer (5′-AAGCTTTTTTTTTTTN-3′, N=C, G, ...
    ... Northern blot analysis Total Poly(A) + RNA from 13- and 33-week-old human fetal cerebrum (Biochain) was electrophoresed in a 1% agarose gel containing 0.66 M formaldehyde and was blotted onto a ...

  • Ramesh Ramakrishnan, David Dorris, Anna Lublinsky, Allen Nguyen, Marc Domanus, Anna Prokhorova, Linn Gieser, Edward Touma, Randall Lockner, Murthy Tata, Xiaomei Zhu, Marcus Patterson, Richard Shippy, Timothy J. Sendera, and Abhijit Mazumder
    An assessment of Motorola CodeLinkTM microarray performance for gene expression profiling applications
    Nucleic Acids Res., Apr 2002; 30: 30.
    ... MATERIALS AND METHODS Target preparation Five micrograms of total RNA (BioChain, Hayward, CA) were added to a reaction mix in a final volume of 12 µl, ...
    ... compared within three human poly(A)+ RNA samples (heart lot#A403084, brain lot#A311144 and kidney lot#A404013 from BioChain) using the Motorola CodeLink(TM) UniSet Human 1 expression bioarrays and the ABI TaqMan (R) products. ...

  • Antonio Riccio, Cesar Mattei, Rosemary E. Kelsell, Andrew D. Medhurst, Andrew R. Calver, Andrew D. Randall, John B. Davis, Christopher D. Benham, and Menelas N. Pangalos
    Cloning and Functional Expression of Human Short TRP7, a Candidate Protein for Store-operated Ca2+ Influx
    J. Biol. Chem., Mar 2002; 277: 12302 - 12309. 
    ... David Netherland's Brain Bank, Amsterdam) or purchased as prepared RNA from Biochain (San Leandro, CA) and CLONTECH.

  • Sridhar Ramaswamy, Pablo Tamayo, Ryan Rifkin, Sayan Mukherjee, Chen-Hsiang Yeang, Michael Angelo, Christine Ladd, Michael Reich, Eva Latulippe, Jill P. Mesirov, Tomaso Poggio, William Gerald, Massimo Loda, Eric S. Lander, and Todd R. Golub
    Multiclass cancer diagnosis using tumor gene expression signatures
    PNAS, Dec 2001; 98: 15149 - 15154. 
    ... Normal tissue RNA (Biochain, Hayward, CA) was from snap-frozen autopsy specimens collected through the International Tissue Collection Network.

  • Mariana Nacht, Tatiana Dracheva, Yuhong Gao, Takeshi Fujii, Yidong Chen, Audrey Player, Viatcheslav Akmaev, Brian Cook, Michael Dufault, Mindy Zhang, Wen Zhang, MingZhou Guo, John Curran, Sean Han, David Sidransky, Kenneth Buetow, Stephen L. Madden, and Jin Jen
    Molecular characteristics of non-small cell lung cancer
    PNAS, Dec 2001; 98: 15203 - 15208.
    ... Tumor RNA samples used for quantitative PCR and GeneChip analyses were either purchased from BioChain (Hayward, CA) or obtained in the same manner as samples used for SAGE ( ). ...

  • Makiko Iguchi, Setsuya Aiba, Yumiko Yoshino, and Hachiro Tagami
    Human Follicular Papilla Cells Carry Out Nonadipose Tissue Production of Leptin
    J. Invest. Dermatol., Dec 2001; 117: 1349 - 1356. 
    ... We also used RNA from human cutaneous adipose tissue (BioChain Institute, Hayward, CA) as a control

  • John E. DeHaven, Katherine A. Robinson, Bryce A. Nelson, and Maria G. Buse
    A Novel Variant of Glutamine: Fructose-6-Phosphate Amidotransferase-1 (GFAT1) mRNA Is Selectively Expressed in Striated Muscle
    Diabetes, Nov 2001; 50: 2419 - 2424. (cat. no. 061015) and human whole brain total RNA (cat. no. 061002) was obtained from Biochain Institute (Hayward, CA). ...

  • Feng Wang-Johanning, Andra R. Frost, Gary L. Johanning, M. B. Khazaeli, Albert F. LoBuglio, Denise R. Shaw, and Theresa V. Strong
    Expression of Human Endogenous Retrovirus K Envelope Transcripts in Human Breast Cancer
    Clin. Cancer Res., Jun 2001; 7: 1553 - 1560. 
    ... and RNA from breast cancer tissues (invasive ductal carcinoma) was purchased from BioChain Institute, Inc. ...

  • Huibin Yue, P. Scott Eastman, Bruce B. Wang, James Minor, Michael H. Doctolero, Rachel L. Nuttall, Robert Stack, John W. Becker, Julie R. Montgomery, Marina Vainer, and Rick Johnston
    An evaluation of the performance of cDNA microarrays for detecting changes in global mRNA expression
    Nucleic Acids Res., Apr 2001; 29: 41.
    ... Oligotex resin (Qiagen, Valencia, CA) from commercially available human placenta, brain and heart total RNA (Biochain, San Leandro, CA). ...
    ... done in the presence of 200 ng poly(A) mRNA, from either human brain or heart (Biochain, Hayward, CA). ...

  • Yuan Zhu, David Michalovich, Hsiao-Ling Wu, K. B. Tan, George M. Dytko, Ishrat J. Mannan, Rogely Boyce, James Alston, Lauren A Tierney, Xiaotong Li, Nicole C. Herrity, Lisa Vawter, Henry M. Sarau, Robert S. Ames, Colleen M. Davenport, J. Paul Hieble, Shelagh Wilson, Derk J. Bergsma, and Laura R. Fitzgerald
    Cloning, Expression, and Pharmacological Characterization of a Novel Human Histamine Receptor
    Mol. Pharmacol., Mar 2001; 59: 434 - 441. 
    ... Ravid in Netherland's Brain Bank (Amsterdam, the Netherlands), or purchased as preprepared RNA from Biochain (San Leandro, CA), CLONTECH (Palo Alto, CA). ...

  • John B. Welsh, Patrick P. Zarrinkar, Lisa M. Sapinoso, Suzanne G. Kern, Cynthia A. Behling, Bradley J. Monk, David J. Lockhart, Robert A. Burger, and Garret M. Hampton
    Analysis of gene expression profiles in normal and neoplastic ovarian tissue samples identifies candidate molecular markers of epithelial ovarian cancer
    PNAS, Jan 2001; 98: 1176 - 1181.
    ... RNA from normal human ovarian tissue was purchased from BioChain Institute (Hayward, CA). ...
    ... Normal human ovary purchased from BioChain was not enriched for any specific cell type before RNA preparation, and, accordingly, we observed...

  • Kenneth R. Luehrsen, Scott Davidson, Yun Ji Lee, Riaz Rouhani, Ali Soleimani, Teresa Raich, Carol A. Cain, Ellen J. Collarini, Douglas T. Yamanishi, Jennifer Pearson, Kerry Magee, Mary Rose Madlansacay, Veeraiah Bodepudi, David Davoudzadeh, Paula A. Schueler, and Walt Mahoney
    High-density Hapten Labeling and HRP Conjugation of Oligonucleotides for Use as In Situ Hybridization Probes to Detect mRNA Targets in Cells and Tissues
    J. Histochem. Cytochem., Jan 2000; 48: 133 - 146. 
    ... Total RNA from human adult peripheral blood was purchased from Biochain Institute (San Leandro, CA). ...

  • Adrienne E. Dubin, Rene Huvar, Michael R. D'Andrea, Jayashree Pyati, Jessica Y. Zhu, K. C. Joy, Sandy J. Wilson, Jose E. Galindo, Charles A. Glass, Lin Luo, Michael R. Jackson, Timothy W. Lovenberg, and Mark G. Erlander
    The Pharmacological and Functional Characteristics of the Serotonin 5-HT3A Receptor Are Specifically Modified by a 5-HT3B Receptor Subunit
    J. Biol. Chem., Oct 1999; 274: 30799 - 30810. 
    ... Total RNA from normal human ascending and descending colon (CDP-061068; lot 8906066; CDP-061069; lot 8906067; BioChain Institute, Inc.) and 5 µg of each poly(A) RNA from normal human small intestine and ...

  • Bo Hu, Kien Trinh, William F. Figueira, and Paul A. Price
    Isolation and Sequence of a Novel Human Chondrocyte Protein Related to Mammalian Members of the Chitinase Protein Family
    J. Biol. Chem., Aug 1996; 271: 19415 - 19420. 
    ... total RNA from human heart, brain, kidney, liver, lung, pancreas, and spleen was purchased from BioChain Institute, Inc. ...

  • Ilgar Abbaszade, Rui-Qin Liu, Fude Yang, Stuart A. Rosenfeld, O. Harold Ross, John R. Link, Dawn M. Ellis, Micky D. Tortorella, Michael A. Pratta, Jeannine M. Hollis, Richard Wynn, Jodie L. Duke, Henry J. George, Milton C. Hillman, Jr., Kathleen Murphy, Barbara H. Wiswall, Robert A. Copeland, Carl P. Decicco, Robert Bruckner, Hideaki Nagase, Yoshifumi Itoh, Robert C. Newton, Ronald L. Magolda, James M. Trzaskos, Gregory F. Hollis, Elizabeth C. Arner, and Timothy C. Burn
    Cloning and Characterization of ADAMTS11, an Aggrecanase from the ADAMTS Family
    J. Biol. Chem., Aug 1999; 274: 23443 - 23450. 
    ... RNAs from normal and diseased tissues were obtained from a commercial vendor (Biochain Institute Inc., San Leandro, CA) with quality being assessed in ethidium bromide-stained agarose gels prior ...

 


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