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Tissue
Section:
-
Mary Ellen Maddalena, Jaclyn Fox, Jianqing Chen, Weiwei
Feng, Aldo Cagnolini, Karen E. Linder, Michael F. Tweedle,
Adrian D. Nunn, and Laura E. Lantry
177Lu-AMBA Biodistribution,
Radiotherapeutic Efficacy, Imaging, and Autoradiography in
Prostate Cancer Models with Low GRP-R Expression
 J.
Nucl. Med., Dec 2009; 50: 2017 - 2024.
 ...
...controls were frozen sections of human prostate carcinoma
(Biochain). To establish the growing fraction of the
tumors, immunohistochemistry...Positive controls were human
breast carcinoma frozen sections (Biochain). The
immunohistochemistry slides were graded in a range from...
...
Ref. link
-
Karen J. Meaburn, Prabhakar R. Gudla, Sameena Khan, Stephen
J. Lockett, and Tom Misteli
Disease-specific gene repositioning in breast cancer
J.
Cell Biol., Dec 2009; 187: 801 - 812.
...
...Normal Absence of cancer; 56 y BioChain T2234086 5
microm N6 Normal NAT; 50 y BioChain TMA core A2 4
microm N7 Normal...C7 IDC TMN stage T4N3M1; 50 y BioChain
TMA core C1 4 microm C8 IDC... ...
Ref. link
-
Caterina Bianco, Catherine Cotten, Enza Lonardo, Luigi
Strizzi, Christina Baraty, Mario Mancino, Monica Gonzales,
Kazuhide Watanabe, Tadahiro Nagaoka, Colin Berry, Andrew E.
Arai, Gabriella Minchiotti, and David S. Salomon
Cripto-1 Is Required for Hypoxia to Induce Cardiac
Differentiation of Mouse Embryonic Stem Cells
Am.
J. Pathol., Nov 2009; 175: 2146 - 2158.
...
...Porcine Heart Tissue Sections Paraffin and frozen tissue
sections from patients who suffered acute MI (AMI) were
purchased from Biochain Institute (Hayward, CA):
eight AMI serial human heart tissue sections and six non-AMI
(congenital heart disease or hypertension... ...
Ref. link
-
Tomomi Ueki, Jae-Hyun Park, Toshihiko Nishidate, Kyoko
Kijima, Koichi Hirata, Yusuke Nakamura, and Toyomasa
Katagiri
Ubiquitination and Downregulation of BRCA1 by
Ubiquitin-Conjugating Enzyme E2T Overexpression in Human
Breast Cancer Cells
Cancer
Res., Nov 2009; 69: 8752 - 8760.
...
...analysis Slides of paraffin-embedded breast cancer
tissues and other normal human tissues (lung, heart, liver,
and kidney; Biochain) were processed for antigen
retrieval by the antigen retrieval solution pH9 (DAKO
Cytomation), and treated with peroxidase... ...
Ref. link
-
Sergey A. Shiryaev, Albert G. Remacle, Alexei Y. Savinov,
Andrei V. Chernov, Piotr Cieplak, Ilian A. Radichev, Roy
Williams, Tatiana N. Shiryaeva, Katarzyna Gawlik, Tatiana I.
Postnova, Boris I. Ratnikov, Alexei M. Eroshkin, Khatereh
Motamedchaboki, Jeffrey W. Smith, and Alex Y. Strongin
Inflammatory Proprotein Convertase-Matrix
Metalloproteinase Proteolytic Pathway in Antigen-presenting
Cells as a Step to Autoimmune Multiple Sclerosis
J.
Biol. Chem., Oct 2009; 284: 30615 - 30626.
...
...oblongata of healthy and MS patients were purchased from
the BioChain Institute (Hayward, CA). The frozen
human brain tissue samples...blotting with the MBP antibody.
The extracts were purchased from BioChain Institute
(Hayward, CA). The representative samples are shown... ...
Ref. link
-
Stefan A. Kaden, Stefanie Kurig, Katrin Vasters, Kay
Hofmann, Kurt S. Zaenker, Juergen Schmitz, and Gregor
Winkels
Enhanced Dendritic Cell-Induced Immune Responses
Mediated by the Novel C-Type Lectin Receptor mDCAR1
J.
Immunol., Oct 2009; 183: 5069 - 5078.
...
...R35-95), rIgG2b (A95-1), all from BD Biosciences.
Immunofluorescence microscopy Frozen splenic tissue sections
(5-10 um; BioChain)
derived from BALB/c mice were incubated for 15 h at 4C with
the following mAb conjugates diluted in PBS containing 1%
BSA... ...
Ref. link
-
Jeanne L. Theis, J. Martijn Bos, Jason D. Theis, Dylan V.
Miller, Joseph A. Dearani, Hartzell V. Schaff, Bernard J.
Gersh, Steve R. Ommen, Richard L. Moss, and Michael J.
Ackerman
Expression Patterns of Cardiac Myofilament Proteins:
Genomic and Protein Analysis of Surgical Myectomy Tissue
From Patients With Obstructive Hypertrophic Cardiomyopathy
Circ
Heart Fail, Jul 2009; 2: 325 - 333.
...
...Healthy flash-frozen heart tissue was obtained from the
interventricular septum of a disease-free, accidental death
victim (Biochain,
Hayward, Calif). Healthy tissue was flash frozen in liquid
nitrogen, to compare protein levels with the HCM myectomy
tissue... ...
Ref. link
-
TEODORA NIKOLOVA, MARKUS CHRISTMANN, and BERND KAINA
FEN1 is Overexpressed in Testis, Lung and Brain
Tumors
Anticancer
Res, Jul 2009; 29: 2453 - 2459.
...
...glioblastoma multiforme (n=13) and astrocytoma (n=7)]
were compared with commercially available extracts from
normal human cerebellum (BioChain,
Hayward, CA, USA). For preparation of total tumor tissue
extracts, the frozen tissue was crushed in a mortar and
transferred... ...
Ref. link
-
Tadashi Matsumoto, Kazuhiro Minegishi, Hitoshi Ishimoto,
Mamoru Tanaka, Jon D. Hennebold, Takahide Teranishi,
Yoshihisa Hattori, Masataka Furuya, Takayuki Higuchi,
Satoshi Asai, Seon Hye Kim, Kei Miyakoshi, and Yasunori
Yoshimura
Expression of Ovary-Specific Acidic Protein in
Steroidogenic Tissues: A Possible Role in Steroidogenesis
Endocrinology,
Jul 2009; 150: 3353 - 3359.
...
...derived from human and mouse tissues were purchased from
CLONTECH Laboratories (Mountain View, CA), Zyagen (San
Diego, CA), and BioChain
(Hayward, CA). Reverse transcription reactions with random
primers were carried out with Omniscript reverse
transcriptase... ...
Ref. link
-
Barry W. Larman, Michele J. Karolak, Derek C. Adams, and
Leif Oxburgh
Chordin-like 1 and Twisted Gastrulation 1 Regulate
BMP Signaling following Kidney Injury
J.
Am. Soc. Nephrol., May 2009; 20: 1020 - 1031.
...
...Technology and goat anti-rabbit Alexa Fluor 568
(Molecular Probes). Formalin-fixed human kidney sections
were purchased from the Biochain
Institute (Hayward, CA) and Zyagen Laboratories (San Diego,
CA). Proximal tubules and nuclei were marked as described
already... ...
Ref. link
-
Ayuko A. Iverson, Cheryl Gillett, Paul Cane, Christopher D.
Santini, Thomas M. Vess, Lauren Kam-Morgan, Alice Wang,
Marcia Eisenberg, Charles M. Rowland, Janice J. Hessling,
Samuel E. Broder, John J. Sninsky, Andrew Tutt, Steven
Anderson, and Sheng-Yung P. Chang
A Single-Tube Quantitative Assay for mRNA Levels of
Hormonal and Growth Factor Receptors in Breast Cancer
Specimens
J.
Mol. Diagn., Mar 2009; 11: 117 - 130.
...
...determine FFPE section-to-section reproducibility, five
sequential sections from each of 10 breast cancer tumor FFPE
samples (BioChain
Institute, Hayward, CA) were obtained. Before RNA was
isolated, the slide was checked to ensure that all sections
from each... ...
Ref. link
-
Ke
Zen, Dan-Qing Liu, Li-Min Li, Celia X-J Chen, Ya-Lan Guo,
Bihn Ha, Xi Chen, Zhen-Yu Zhang, and Yuan Liu
The Heparan Sulfate Proteoglycan Form of Epithelial
CD44v3 Serves as a CD11b/CD18 Counter-receptor during
Polymorphonuclear Leukocyte Transepithelial Migration
J.
Biol. Chem., Feb 2009; 284: 3768 - 3776.
...
...on coverslides followed by air drying before
immunostaining was performed. Normal human colon sections
were purchased from Biochain
(Hayward, CA). Recombinant human TNF-alpha and
interferon-gamma were purchased from Upstate Biotechnology
and used at 20 and... ...
Ref. link
-
Liliane Benkoël, Jean-Paul Bernard, Marie-Jos?Payan-Defais,
Lydie Crescence, Cécile Franceschi, Mireille Delmas, Mehdi
Ouaissi, Bernard Sastre, Jos?Sahel, Anne-Marie Benoliel,
Pierre Bongrand, Françoise Silvy, Laurent Gauthier, François
Romagn? Dominique Lombardo, and Eric Mas
Monoclonal antibody 16D10 to the COOH-terminal
domain of the feto-acinar pancreatic protein targets
pancreatic neoplastic tissues
Mol.
Cancer Ther., Feb 2009; 8: 282 - 291.
...
...Frozen tissue slides of a PDAC provided by
BioChain were also
studied. Adjacent nontumoral...paraffin-embedded sections.
Tissue provided by BioChain.
All controls and patients were Caucasians...studied as
negative tissue controls (BioChain).
Formalin-fixed, paraffin-embedded tissue... ...
Ref. link
-
Adam Palermo, Regis Doyonnas, Nidhi Bhutani, Jason Pomerantz,
Ozan Alkan, and Helen M. Blau
Nuclear reprogramming in heterokaryons is rapid,
extensive, and bidirectional
FASEB
J, Jan 2009; 10.1096/fj.08-122903.
...
...Universal Total RNA (SuperArray, Frederick, MD, USA).
Mouse reference RNA was a mixture of Universal RNA: Mouse
Normal Tissues (BioChain,
Hayward, CA, USA), Universal Mouse Reference RNA (Strat-agene,
La Jolla, CA, USA), and RNA from whole mouse E17.5
embryos... ...
Ref. link
-
Katherine Elena Varley and Robi David Mitra
Nested Patch PCR enables highly multiplexed mutation
discovery in candidate genes
Genome
Res., Nov 2008; 18: 1844 - 1850.
...
...tissue from an 81-yr-old male was obtained from
Biochain (
www.biochain.com ),
catalog no. D8235090-PP-10. Targets were...either the colon
tumor or the adjacent normal tissue (Biochain,
catalog no. D8235090-PP-10). This reaction was... ...
Ref. link
-
Masashi Akiyama, Kaori Sakai, Chitoshi Takayama, Teruki
Yanagi, Yasuko Yamanaka, James R. McMillan, and Hiroshi
Shimizu
CGI-58 Is an
 /¦Â-Hydrolase
within Lipid Transporting Lamellar Granules of
Differentiated Keratinocytes
Am.
J. Pathol., Nov 2008; 173: 1349 - 1360.
...
...tissue slides of human liver and brain were purchased
from BioChain Institute Inc.
(Hayward, CA). Normal human adult liver or brain...normal
mouse and human tissue extracts were purchased from
BioChain Institute, Inc.
(Hayward, CA). Cell lysates (100 ug of protein... ...
Ref. link
-
Lori M. Roberts, Kathleen Woodford, Mei Zhou, Deborah S.
Black, Jill E. Haggerty, Emily H. Tate, Kent K. Grindstaff,
Wondwessen Mengesha, Chandrasekaran Raman, and Noa Zerangue
Expression of the thyroid hormone transporters MCT8
(SLC16A2) and OATP14 (SLCO1C1) at the blood-brain barrier
Endocrinology,
Aug 2008; 10.1210/en.2008-0378.
...
...parietal lobe (female, 37 weeks), hippocampus (female, 82
years), and choroid plexus (female, 70 years) were purchased
from BioChain Institute
(Hayward, CA, USA). Quantitative PCR RNeasy RNA Isolation
Kit and Qiashredder homogenizer columns (Qiagen, Valencia...
...
Ref. link
-
Jing Chen, Jean Lozach, Eliza Wickham Garcia, Bret Barnes,
Shujun Luo, Ivan Mikoulitch, Lixin Zhou, Gary Schroth, and
Jian-Bing Fan
Highly sensitive and specific microRNA expression
profiling using BeadArray technology
Nucleic
Acids Res., Aug 2008; 36: e87.
...
...experiments, small RNA molecules were enriched using
Invitrogen's PureLink miRNA Isolation Kit. FFPE samples were
purchased from Biochain Institute,
Inc., and RNA was extracted using Qiagen's RNeasy
FFPE Kit. miRNA profiling on universal BeadArray platform...
...
Ref. link
-
Yasunaga Ono, Naohisa Oda, Shin Ishihara, Atsushi Shimomura,
Nobuki Hayakawa, Atsushi Suzuki, Akihiko Horiguchi, Takao
Senda, Shuichi Miyakawa, and Mitsuyasu Itoh
Insulinoma cell calcium-sensing receptor influences
insulin secretion in a case with concurrent familial
hypocalciuric hypercalcemia and malignant metastatic
insulinoma.
Eur.
J. Endocrinol., Jul 2008; 159: 81 - 86.
...
...for both pancreatic and kidney tissues were purchased
from BioChain Institute Inc.
(Hayward, CA, USA). RT-PCR for the detection...thickness
were prepared. Section of parathyroid purchased from
BioChain Institute Inc.
was used as a positive control. Immunohistochemistry... ...
Ref. link
-
Kevin A. Glenn, Rick F. Nelson, Hsiang M. Wen, Adam J.
Mallinger, and Henry L. Paulson
Diversity in Tissue Expression, Substrate Binding,
and SCF Complex Formation for a Lectin Family of Ubiquitin
Ligases
J.
Biol. Chem., May 2008; 283: 12717 - 12729.
...
...and pelleting debris by centrifugation at 16,000 g, 4 C
for 15 min. Mouse tissue preparations from a commercial
supplier (BioChain,
Hayward, CA) produced similar results. Immunoprecipitation
(IP) Nondenatured lysates from 10-cm plates were
incubated... ...
Ref. link
-
Eric Soupene, Vladimir Serikov, and Frans A. Kuypers
Characterization of an acyl-coenzyme A binding
protein predominantly expressed in human primitive
progenitor cells
J.
Lipid Res., May 2008; 49: 1103 - 1112.
...
...protein array (human adult normal tissues
Biochain Institute, Inc.)
was performed as follows...according to the manufacturers
instructions (Biochain Institute,
Inc.). Immunohistochemistry...antibody on a
MegaWestern protein array (Biochain Institute, Inc.)
representing 30 different... ...
Ref. link
-
Kathryn B. Horwitz, Wendy W. Dye, Joshua Chuck Harrell,
Peter Kabos, and Carol A. Sartorius
Rare steroid receptor-negative basal-like
tumorigenic cells in luminal subtype human breast cancer
xenografts
PNAS,
Apr 2008; 105: 5774 - 5779.
...
...Cancerous Breast Tis- sue. Normal human breast tissue was
obtained from US Biomax. Breast cancer tissue arrays were
purchased from Biochain.
These were stained using a DAB system for CK5 and PR as
described. Were also stained fluorescently for CK5 and PR.
For analyses... ...
Ref. link
-
Melissa L. Palmer, Katherine R. Schiller, and Scott M.
O'Grady
Apical SK potassium channels and Ca2+-dependent
anion secretion in endometrial epithelial cells
J.
Physiol., Feb 2008; 586: 717 - 726.
...
...DNase-free RNA isolated from porcine brain was purchased
from BioChain Institute Inc.
(Hayward, CA, USA). Dulbeccos modified Eagles...DNase-free
RNA isolated from porcine brain was purchased from
BioChain Institute Inc.
(Hayward, CA, USA). Dulbecco's modified Eagle's...
...
Ref. Link
-
David A. Taylor-Fishwick, Angela Bowman, MariCarmen Korngiebel-Rosique, and Aaron I. Vinik
Pancreatic Islet Immunoreactivity to the Reg Protein INGAP
J. Histochem. Cytochem., Feb 2008; 56: 183 - 191.
... ...Committee, Eastern Virginia Medical School. Immunohistochemistry Monkey, human, and rat pancreas sections were supplied by Biochain (Hayward, CA). Harvested mouse pancreata were fixed in 10% formalin (Fisher Chemicals Fair Lawn, NJ) and paraffin embedded... ...
Ref. link
-
Jascha-N. Rybak, Christoph Roesli, Manuela Kaspar, Alessandra Villa, and Dario Neri
The Extra-domain A of Fibronectin Is a Vascular Marker of Solid Tumors and Metastases
Cancer Res., Nov 2007; 67: 10948 - 10957.
... ...of freshly frozen tissue specimens. In many cases, freshly frozen tissue sections in microarray format were obtained from BioChain. To verify successful in vivo biotinylation, staining of biotinylated structures was done as described in ref. (15) using streptavidin... ...
Ref. link
-
Mingyan Zhou, Li Xia, and Joanne Wang
Metformin Transport by a Newly Cloned Proton-Stimulated Organic Cation Transporter (Plasma Membrane Monoamine Transporter) Expressed in Human Intestine
Drug Metab. Dispos., Oct 2007; 35: 1956 - 1962.
... ...localization of PMAT in human small intestine, commercial slides containing human small intestine tissue were purchased from BioChain Institute Inc. (Hayward, CA). Slides were rinsed twice with PBS and fixed for 30 min at room temperature with 4% (v/v) paraformaldehyde... ...
Ref. link
-
Yasuna Kobayashi, Ayumi Tsuchiya, Tomofumi Hayashi, Noriko Kohyama, Masayuki Ohbayashi, and Toshinori Yamamoto
Isolation and Characterization of Polyspecific Mouse Organic Solute Carrier Protein 1 (mOscp1)
Drug Metab. Dispos., Jul 2007; 35: 1239 - 1245.
... ...2005). A 5-mum wax section of mouse testis was obtained from BioChain Institute, Inc. (Hayward, CA) and sectioning was carried out...2005). A 5- m wax section of mouse testis was obtained from BioChain Institute, Inc. (Hayward, CA) and sectioning was carried out... ...
Ref. link
-
Masayo Hosokawa, Akio Takehara, Koichi Matsuda, Hidetoshi Eguchi, Hiroaki Ohigashi, Osamu Ishikawa, Yasuhisa Shinomura, Kohzoh Imai, Yusuke Nakamura, and Hidewaki Nakagawa
Oncogenic Role of KIAA0101 Interacting with Proliferating Cell Nuclear Antigen in Pancreatic Cancer
Cancer Res., Mar 2007; 67: 2568 - 2576.
... ...Cancer and Cardiovascular Diseases under the appropriate informed consent. Sections from normal pancreas were purchased from Biochain (Hayward, CA). The sections were deparaffinized and autoclaved at 108C in DakoCytomation Target Retrieval Solution High pH... ...
Ref. link
-
Ke Zen, Celia X.-J. Chen, Yi-Tien Chen, Rosemarie Wilton, and Yuan Liu
Receptor for Advanced Glycation Endproducts Mediates Neutrophil Migration across Intestinal Epithelium
J. Immunol., Feb 2007; 178: 2483 - 2490.
... ...coverslides followed by air-drying before immunostaining was performed. Normal human colon sections were also purchased from Biochain. A mouse monoclonal (IgG2b) and a goat polyclonal Ab against human RAGE were obtained from RD Systems. A high-titer polyclonal... ...
Ref. link
-
Liang-Ru Zhu, Paul E. Thomas, Gang Lu, Kenneth R. Reuhl, Guang-Yu Yang, Li-Dong Wang, Shou-Lin Wang, Chung S. Yang, Xiao-Yang He, and Jun-Yan Hong
CYP2A13 in Human Respiratory Tissues and Lung Cancers: An Immunohistochemical Study with A New Peptide-Specific Antibody
Drug Metab. Dispos., Oct 2006; 34: 1672 - 1676.
... ...human trachea (T2234160), bronchus (HuFPT111), and lung (containing bronchiole areas, HuPFT131 and T2234152) were obtained from Biochain Institute, Inc. (Hayward, CA) and US Biomax, Inc. (Rockville, MD). Tissue microarrays containing normal lung alveolar tissues... ...
Ref. link
-
Sara J. Bowne, Qin Liu, Lori S. Sullivan, Jingya Zhu, Catherine J. Spellicy, Catherine Bowes Rickman, Eric A. Pierce, and Stephen P. Daiger
Why Do Mutations in the Ubiquitously Expressed Housekeeping Gene IMPDH1 Cause Retina-Specific Photoreceptor Degeneration?
Invest. Ophthalmol. Vis. Sci., Sep 2006; 47: 3754 - 3765.
... ...additional human tissues was purchased from BioChain (Hayward, CA). Total RNA (20 Mg) from...peripheral blood leukocyte first-strand cDNA (BioChain) using the following primers: 5-gacgacgacaagatggccgactacctgattagtgg-3...human retinal cDNA was purchased from BioChain. Retinal transcripts were analyzed with... ...
Ref. link
- Liang-Ru
Zhu, Paul E. Thomas, Gang Lu, Kenneth R. Reuhl, Guang-Yu
Yang, Li-Dong Wang, Shou-Lin Wang, Chung S. Yang, Xiao-Yang
He, and Jun-Yan Hong
CYP2A13 in Human Respiratory Tissues and Lung Cancers:
An Immunohistochemical Study with A New Peptide-specific
Antibody
Drug
Metab. Dispos., Jun 2006; 10.1124/dmd.106.011049.
...
...trachea (T2234160), bronchus (HuFPT111) and lung (containing
bronchiole areas, HuPFT131 and T2234152) were obtained from
BioChain Institute,
Inc. (Hayward, CA)... ...
- Masato
Otsuka, Takuya Matsumoto, Riyo Morimoto, Shigeo Arioka,
Hiroshi Omote, and Yoshinori Moriyama
From the Cover: A human transporter protein that mediates
the final excretion step for toxic organic cations
PNAS,
Dec 2005; 102: 17923 - 17928.
...
...was performed subsequently as described (12). Immunohistochemistry.
Human paraffin tissue sections were obtained from BioChain.
Immunohistochemical analysis was performed by the HRP-DAB
method or indirect immunofluorescence microscopy as described...
...
- Hiroki
Miyazaki, Naohiko Anzai, Sophapun Ekaratanawong, Takeshi
Sakata, Ho Jung Shin, Promsuk Jutabha, Taku Hirata, Xin
He, Hiroshi Nonoguchi, Kimio Tomita, Yoshikatsu Kanai, and
Hitoshi Endou
Modulation of Renal Apical Organic Anion Transporter
4 Function by Two PDZ Domain–Containing Proteins
J.
Am. Soc. Nephrol., Dec 2005; 16: 3498 - 3506.
...
...primers used for PCR amplification are shown in Table
1. Immunohistochemical Analysis We used human single-tissue
slides (BioChain) for
light microscopic immunohistochemical analysis, and they
were treated with 10 Mg/ml primary rabbit polyclonal antibodies...
...
- Yasuna
Kobayashi, Akiko Shibusawa, Hironori Saito, Naomi Ohshiro,
Masayuki Ohbayashi, Noriko Kohyama, and Toshinori Yamamoto
Isolation and Functional Characterization of a Novel
Organic Solute Carrier Protein, hOSCP1
J.
Biol. Chem., Sep 2005; 280: 32332 - 32339.
...
...cysteine and the 14 amino acids of the COOH terminus
of hOSCP1. A 5-mum wax section of human placenta was obtained
from BioChain Institute,
Inc. (San Leandro, CA), and light microscopic immunohistochemical
analysis was carried out using the streptavidin-biotin-horseradish...
...
- Tsukasa
Kawahara, Darren Ritsick, Guangjie Cheng, and J. David Lambeth
Point Mutations in the Proline-rich Region of p22phox
Are Dominant Inhibitors of Nox1- and Nox2-dependent Reactive
Oxygen Generation
J.
Biol. Chem., Sep 2005; 280: 31859 - 31869.
...
...Thermo Shandon Cryotome) and placed on glass microscope
slides. Tissue slides of the human kidney cortex were purchased
from BioChain. Nox5
cDNA was amplified by PCR of human spleen cDNA library (BD
Biosciences) using a set of primers (5-GCATGGATCCACCATGAACACATCTGGAGACCCA-3...
...
- Mindy
A. Maynard, Andrew J. Evans, Tomoko Hosomi, Shuntaro Hara,
Michael A. S. Jewett, and Michael Ohh
Human HIF-34 is a dominant-negative regulator of HIF-1
and is down-regulated in renal cell carcinoma
FASEB
J, Sep 2005; 19: 1396 - 1406.
... ...based on the predicted HIF-3a4 cDNA (accession no.
BC026308), and PCR was performed using human cerebellum
cDNA library (BioChain
Institute, San Leandro, CA, USA). The amplified fragment
was cloned into pGEM-T Easy vector (Promega, Madison, WI,
USA... ...
- Masanori
Onda, Mark Willingham, Satoshi Nagata, Tapan K. Bera, Richard
Beers, Mitchell Ho, Raffit Hassan, Robert J. Kreitman, and
Ira Pastan
New Monoclonal Antibodies to Mesothelin Useful for Immunohistochemistry,
Fluorescence-Activated Cell Sorting, Western Blotting, and
ELISA
Clin.
Cancer Res., Aug 2005; 11: 5840 - 5846.
... ...peroxidase immunohistochemical results are shown.
Paraffin-embedded tissue sections (as shown in Table 2)
were obtained from BioChain
Institute (Hayward, CA) and treated with a citraconic anhydride
antigen retrieval procedure (23). The complete results...
...
- Akira
Togashi, Toyomasa Katagiri, Shingo Ashida, Tomoaki Fujioka,
Osamu Maruyama, Yoshiaki Wakumoto, Yoshiro Sakamoto, Makoto
Fujime, Yoshio Kawachi, Taro Shuin, and Yusuke Nakamura
Hypoxia-Inducible Protein 2 (HIG2), a Novel Diagnostic
Marker for Renal Cell Carcinoma and Potential Target for
Molecular Therapy
Cancer
Res., Jun 2005; 65: 4817 - 4826.
...
...Paraffin-embedded specimens of normal human tissues (heart,
liver, lung, adult kidney, fetal kidney, prostate, pancreas,
spinal cord; BioChain,
Hayward, CA) were treated with xylene and ethanol to remove
the paraffin. We used ENVISION+ Kit/HRP (DakoCytomation,
Kyoto... ...
- Tetsuo
Mashima, Tomoko Oh-hara, Shigeo Sato, Mikiko Mochizuki,
Yoshikazu Sugimoto, Kanami Yamazaki, Jun-ichi Hamada, Mitsuhiro
Tada, Tetsuya Moriuchi, Yuichi Ishikawa, Yo Kato, Hiroshi
Tomoda, Takao Yamori, and Takashi Tsuruo
p53-Defective Tumors With a Functional Apoptosome-Mediated
Pathway: A New Therapeutic Target
J
Natl Cancer Inst, May 2005; 97: 765 - 777.
...
...METHODS Materials Normal cell lysates were prepared from
tissues obtained during surgical resection or purchased
from BioChain Institute,
Inc. (San Leandro, CA). Written informed consent was obtained
from those patients (or their guardians) whose... ...
- Keisuke
Taniuchi, Hidewaki Nakagawa, Masayo Hosokawa, Toru Nakamura,
Hidetoshi Eguchi, Hiroaki Ohigashi, Osamu Ishikawa, Toyomasa
Katagiri, and Yusuke Nakamura
Overexpressed P-Cadherin/CDH3 Promotes Motility of Pancreatic
Cancer Cells by Interacting with p120ctn and Activating
Rho-Family GTPases
Cancer
Res., Apr 2005; 65: 3092 - 3099.
...
...Cardiovascular Diseases under the appropriate rules for
informed consent. Tissue sections from normal pancreas were
purchased from BioChain
(Hayward, CA). Tissue-microarray sections of pancreatic
carcinomas (AccuMax Array) were purchased from Petagene
Inc. (Seoul... ...
- Keisuke
Taniuchi, Hidewaki Nakagawa, Toru Nakamura, Hidetoshi Eguchi,
Hiroaki Ohigashi, Osamu Ishikawa, Toyomasa Katagiri, and
Yusuke Nakamura
Down-regulation of RAB6KIFL/KIF20A, a Kinesin Involved
with Membrane Trafficking of Discs Large Homologue 5, Can
Attenuate Growth of Pancreatic Cancer Cell
Cancer
Res., Jan 2005; 65: 105 - 112.
...
Tissue sections from normal pancreas were purchased from
Biochain (Hayward, CA).
...
- F.
Duprat, C. Girard, G. Jarretou, and M. Lazdunski
Pancreatic two P domain K+ channels TALK-1
and TALK-2 are activated by nitric oxide and reactive oxygen
species
J.
Physiol., Jan 2005; 562: 235 - 244.
...
In situ hybridization experiments were performed on adult
human pancreas paraffin-embedded slices purchased from BioChain
(Hayward, CA, USA). ...
- Pierfrancesco Tassone, Victor
S. Goldmacher, Paola Neri, Antonella Gozzini, Masood A.
Shammas, Kathleen R. Whiteman, Linda L. Hylander-Gans, Daniel
R. Carrasco, Teru Hideshima, Reshma Shringarpure, Jialan
Shi, Charles K. Allam, John Wijdenes, Salvatore Venuta,
Nikhil C. Munshi, and Kenneth C. Anderson
Cytotoxic activity of the maytansinoid immunoconjugate
B-B4–DM1 against CD138+ multiple myeloma cells
Blood, Dec 2004; 104: 3688 - 3696.
...
Immunolocalization of B-B4 in human normal tissues Frozen
section Paraffin (1 st sample Biochain,
2 nd Zymed) Tissue No. cases Intensity * cell type or structure
No. cases Intensity ...
- Marina
Bibikova, Joanne M. Yeakley, Eugene Chudin, Jing Chen, Eliza
Wickham, Jessica Wang-Rodriguez, and Jian-Bing Fan
Gene Expression Profiles in Formalin-Fixed, Paraffin-Embedded
Tissues Obtained with a Novel Assay for Microarray Analysis
Clin.
Chem., Dec 2004; 50: 2384 - 2386.
...
samples, we prepared RNA from 5-{mu}m human tissue sections
mounted on microscope slides, obtained from BioChain
Institute according to an Institutional Review Board-approved
protocol. ...
- Mark
Powzaniuk, Sheila McElwee-Witmer, Robert L. Vogel, Tadashi
Hayami, Su Jane Rutledge, Fang Chen, Shun-ichi Harada, Azriel
Schmidt, Gideon A. Rodan, Leonard P. Freedman, and Chang
Bai
The LATS2/KPM Tumor Suppressor Is a Negative Regulator
of the Androgen Receptor
Mol.
Endocrinol., Aug 2004; 18: 2011 - 2023.
...
Paraffin-embedded tissue sections were from BioChain
Institute (Hayward, CA). ...
...
Immunohistochemisty For detection of LATS2, paraffin-embedded
tissue sections (BioChain
Institute) were deparaffinized in xylene, hydrated in graded
ethanol, and treated with Dako target retrieval ...
- MEMBRANE
TRANSPORT, STRUCTURE, FUNCTION, AND BIOGENESIS:
Naohiko Anzai, Hiroki Miyazaki, Rie Noshiro, Suparat Khamdang,
Arthit Chairoungdua, Ho-Jung Shin, Atsushi Enomoto, Shinichi
Sakamoto, Taku Hirata, Kimio Tomita, Yoshikatsu Kanai, and
Hitoshi Endou
The multivalent PDZ domain-containing protein PDZK1
regulates transport activity of renal urate-anion exchanger
URAT1 via its C-terminal
J.
Biol. Chem., Oct 2004; 279: 45942 - 45950.
...
We used human single-tissue slides (Biochain)
for light microscopic immunohistochemical analysis using
the streptavidin-biotin-HRP complex technique (LSAB kit;
DAKO, Carpinteria, CA). ...
...
For the coimmunoprecipitation of endogenous URAT1 and PDZK1,
we used human kidney membrane fractions (Biochain)
and added 13 anti-PDZK1 antibody or control IgG to this
solution. ...
- Pierfrancesco Tassone, Victor
S Goldmacher, Paola Neri, Antonella Gozzini, Masood A Shammas,
Kathleen R Whiteman, Linda Hylander, Daniel R Carrasco,
Teru Hideshima, Reshma Shringarpure, Jialan Shi, Charles
K Allam, John Wijdenes, Salvatore Venuta, Nikhil C Munshi,
and Kenneth C Anderson
Cytotoxic activity of the maytansinoid immunoconjugate
B-B4-DM1 against CD138+ Multiple Myeloma cells
Blood, Aug 2004; 10.1182/blood-2004-03-0963.
...
7 Immunohistochemistry Paraffin multi-tissue arrays were
purchased from Biochain
Institute (Cat. ...
...
I: reactivity of mb B-B4 on human tissues Tissue FROZEN
SECTION PARAFFIN (1 st sample Biochain,
2 nd Zymed) No. cases Intensity*/Cell type or structure
No. cases Intensity*/Cell type or structure ...
- Kazuishi
Kubota, Kaori Nakahara, Toshiaki Ohtsuka, Shuku Yoshida,
Junko Kawaguchi, Yoko Fujita, Yohei Ozeki, Ayako
Hara, Chigusa Yoshimura, Hidehiko Furukawa, Hideyuki Haruyama,
Kimihisa Ichikawa, Makoto Yamashita, Tatsuji Matsuoka, and
Yasuteru Iijima
Identification of 2'-phosphodiesterase, which plays
a role in the 2-5A system regulated by interferon
J. Biol. Chem., Jun 2004; 10.1074/jbc.M400089200.
... 21 kidney and fetal liver were
purchased from Clontech, and human adult colon was from
BioChain Institute.
...
- Tomoaki
Tanaka, Edward T. H. Yeh, and Tetsu Kamitani
NUB1-mediated targeting of the ubiquitin precursor
UbC1 for its C-terminal hydrolysis
Eur. J. Biochem., Mar 2004; 271: 972 - 982.
...
In situ hybridization Paraffin-embedded tissue sections
(thickness, 5{micro}m) of human adult testis were purchased
from BIOCHAIN (Hayward,
CA, USA). ...
- Ryoko Iizuka, Katsuyoshi
Chiba, and Shinobu Imajoh-Ohmi
A Novel Approach for the Detection of Proteolytically
Activated Transglutaminase 1 in Epidermis Using Cleavage
Site-Directed Antibodies
J. Invest. Dermatol., Sep 2003; 121: 457 - 464.
... Immunofluorescence analysis Frozen sections of normal
human skin (BioChain
Institute, Inc., Hayward, California), monolayer-cultured
keratinocytes on collagen-coated cover glasses, and frozen
sections of three-dimensional ...
... In situ TGase assays Frozen sections of normal human
skin (BioChain Institute,
Hayward, CA) were rinsed with 1% (w v) BSA in Tris-buffered
saline, and incubated ...
- Daisuke Kobayashi, Takashi
Nozawa, Kozue Imai, Jun-ichi Nezu, Akira Tsuji, and Ikumi
Tamai
Involvement of Human Organic Anion Transporting Polypeptide
OATP-B (SLC21A9) in pH-Dependent Transport across Intestinal
Apical Membrane
J. Pharmacol. Exp. Ther., Aug 2003; 306: 703 - 708.
... Human adult normal small intestinal tissue slides were
purchased from Biochain
Institute, Inc. ...
- Daisuke Kobayashi, Takashi
Nozawa, Kozue Imai, Jun-ichi Nezu, Akira Tsuji, and Ikumi
Tamai
Involvement of Human Organic Anion Transporting Polypeptide
OATP-B (SLC21A9) in pH-Dependent Transport across Intestinal
Apical Membrane
J.
Pharmacol. Exp. Ther., Apr 2003; 103051300.
... Human adult normal small intestinal tissue slides were
purchased from Biochain
Institute, Inc. ...
- Takehiko Ogura, Tetsushi
Furukawa, Tetsuya Toyozaki, Katsuya Yamada, Ya-Juan Zheng,
Yoshifumi Katayama, Haruaki Nakaya, and Nobuya Inagaki
ClC-3B, a novel ClC-3 splicing variant that interacts
with EBP50 and facilitates expression of CFTR-regulated
ORCC
FASEB
J, Apr 2002; 108451.
... Immunohistochemistry Ready-to-use tissue section slides
of various human tissues (Biochain,
Hayward, CA) were incubated with an anti-human ClC-3B antibody
(1:200) at 4?C in a moist ...
- Hisayuki Nomiyama, Kunio
Hieshima, Takashi Nakayama, Tomonori Sakaguchi, Ryuichi
Fujisawa, Sumio Tanase, Hiroshi Nishiura, Kenjiro Matsuno,
Hiroshi Takamori, Youichi Tabira, Tetsuro Yamamoto, Retsu
Miura, and Osamu Yoshie
Human CC chemokine liver-expressed chemokine/CCL16 is
a functional ligand for CCR1, CCR2 and CCR5, and constitutively
expressed by hepatocytes
Int.
Immunol., Aug 2001; 13: 1021 - 1029.
... Paraffin sections of formaldehyde-fixed normal
human liver obtained from Biochain
Institute (San Leandro, CA) were also dewaxed and rehydrated.
...
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