Promotions

Protein:

• Ruey-Bing Yang, Heng-Kien Au, Chii-Ruey Tzeng, Ming-Tzu Tsai, Ping Wu, Yu-Chih Wu, Thai-Yen Ling, and Yen-Hua Huang
  Characterization of a novel cell-surface protein expressed on human sperm
  Hum. Reprod., Jan 2010; 25: 42 - 51.
  ... ...USA). The PCR products were cloned into pGEM-T Easy plasmid vector (Promega). Human testis tissue lysate was purchased from BioChain Institute, Inc. (Hayward, CA, USA). Human sperm samples were from the Department of Obstetrics and Gynecology, Center for Reproductive... ...
  Ref. link
  


• Hanzhong Liu, Li Liu, Kaifeng Liu, Peyman Bizargity, Wayne W. Hancock, and Gary A. Visner
  Reduced Cytotoxic Function of Effector CD8⁺ T Cells Is Responsible for Indoleamine 2,3-Dioxygenase-Dependent Immune Suppression
  J. Immunol., Jul 2009; 183: 1022 - 1031.
  ... ...pathophysiology Commercial kits were used to determine intracellular ATP level (Molecular Probes), to isolate mitochondrial protein (BioChain Institute) and to assess citrate synthase activity in isolated CD8 T cells according to the manufacturers instruction (13... ...
  Ref. link
  


• Anuradha Chakrabarty, Audrey Blacklock, Stanislav Svojanovsky, and Peter G. Smith
  Estrogen Elicits Dorsal Root Ganglion Axon Sprouting via a Renin-Angiotensin System
  Endocrinology, Jul 2008; 149: 3452 - 3460.
  ... ...antisera preabsorption overnight to a 5-fold excess of blocking peptides for AT2 (Santa Cruz Biotechnology) or REN native protein (BioChain Institute, Inc., Hayward, CA), and primary antisera heat inactivation for 20 min and antibody omission for ACE or AGN. A total... ...
  Ref. link
  


• Jamie Fitzgerald, Cathleen Rich, Fiona H. Zhou, and Uwe Hansen
  Three Novel Collagen VI Chains, 4(VI), 5(VI), and 6(VI)
  J. Biol. Chem., Jul 2008; 283: 20170 - 20180.
  ... ...human adult skeletal muscle for COL6A6 (BioChain Institute). The mouse Col6a4 coding region...structural proteins was obtained commercially (Biochain, catalog number P5244171). A sample was...and a normal adult human tissue panel (Biochain Institute). Sections were blocked with... ...
  Ref. link
  
  
• Eric Soupene, Vladimir Serikov, and Frans A. Kuypers
  Characterization of an acyl-CoA binding protein predominantly expressed in human primitive progenitor cells
  J. Lipid Res., Feb 2008; 10.1194/jlr.M800007-JLR200.
  ... ...protein array (Human adult normal tissues, Biochain Institute, Inc.) was performed as follows...according to the manufacturer's instructions (Biochain Institute, Inc.). Immunohistochemistry...antibody on a MegaWestern protein array (Biochain Institute, Inc.) representing 30 different... ...
  Ref. link
  


• Kevin A. Glenn, Rick F. Nelson, Hsiang M. Wen, Adam J. Mallinger, and Henry L. Paulson
  Diversity in tissue expression, substrate binding and SCF complex formation for a lectin family of ubiquitin ligases
  J. Biol. Chem., Jan 2008; 10.1074/jbc.M709508200.
  ... ...and pelleting debris by centrifugation at 16,000 x g, 4?C for 15 min. Mouse tissue preparations from a commercial supplier (BioChain, Hayward, CA) produced similar results. Immunoprecipitation (IP) Nondenatured lysates from 10 cm plates were incubated with... ...
  Ref. link
  


• Carrie E. Johnson, Yolanda Y. Huang, Amanda B. Parrish, Michelle I. Smith, Allyson E. Vaughn, Qian Zhang, Kevin M. Wright, Terry Van Dyke, Robert J. Wechsler-Reya, Sally Kornbluth, and Mohanish Deshmukh
  Differential Apaf-1 levels allow cytochrome c to induce apoptosis in brain tumors but not in normal neural tissues
  PNAS, Dec 2007; 104: 20820 - 20825.
  ... ...Chemical) along with an ECL-Plus detection system (Amersham Biosciences). Protein array of human astrocytomas was from the BioChain Institute (A1235713-1). Real-Time RT-PCR. RNA was isolated by using the small-scale RNAqueous Kit and treated with DNase... ...
  Ref. link
  


• Yana Zavros, Meghna Waghray, Arthur Tessier, Longchuan Bai, Andrea Todisco, Deborah L. Gumucio, Linda C. Samuelson, Andrzej Dlugosz, and Juanita L. Merchant
  Reduced Pepsin A Processing of Sonic Hedgehog in Parietal Cells Precedes Gastric Atrophy and Transformation
  J. Biol. Chem., Nov 2007; 282: 33265 - 33274.
  ... ...and 60 min. Human Tissue Extracts-Three sets of normal corpus and intestinal-type tumor tissue extracts were purchased from BioChain Institute (Hayward, CA). The tumor tissues supplied were collected from patients with intestinal type gastric cancer. According... ...
  Ref. link
  


• Yana Zavros, Meghna Waghray, Arthur Tessier, Longchuan Bai, Andrea Todisco, Deborah L. Gumucio, Linda C. Samuelson, Andrzej Dlugosz, and Juanita L. Merchant
  Reduced pepsin a processing of sonic hedgehog in parietal cells precedes gastric atrophy and transformation
  J. Biol. Chem., Sep 2007; 10.1074/jbc.M707090200.
  ... ...and 60 min. 5 Human tissue extracts Three sets of normal corpus and intestinal-type tumor tissue extracts were purchased from BioChain Institute (Hayward, CA). Tumor tissues supplied were collected from patients with intestinal type gastric cancer. According... ...
  Ref. link
  


• Scott L. Kominsky, Betty Tyler, Jeffrey Sosnowski, Kelly Brady, Michele Doucet, Delissa Nell, James G. Smedley, III, Bruce McClane, Henry Brem, and Saraswati Sukumar
  Clostridium perfringens Enterotoxin as a Novel-Targeted Therapeutic for Brain Metastasis
  Cancer Res., Sep 2007; 67: 7977 - 7982.
  ... ...glycerol, 5 SDS, and 250 mmol/L Tris-HCl (pH 6.7). Total protein from human brain and spinal cord tissue was obtained from BioChain. Equal amounts of protein from cell and tissue lysates were resolved using 12 SDS-PAGE (Invitrogen). Protein was transferred... ...
  Ref. link
  


• Raghavan Raju, Goran Rakocevic, Ziwei Chen, Gerard Hoehn, Cristina Semino-Mora, Wei Shi, Richard Olsen, and Marinos C. Dalakas
  Autoimmunity to GABAA-receptor-associated protein in stiff-person syndrome
  Brain, Dec 2006; 129: 3270 - 3276.
  ... ...bleed was tested for specificity by enzyme-linked immunosorbent assay (ELISA) and in western blot using total human brain (Biochain, Hayward, CA). Immunoprecipitation and western blot Aliquots of 15 mul serum from SPS or OND control patients were incubated... ...
  Ref. link
  


• Daniel B. Campbell, James S. Sutcliffe, Philip J. Ebert, Roberto Militerni, Carmela Bravaccio, Simona Trillo, Maurizio Elia, Cindy Schneider, Raun Melmed, Roberto Sacco, Antonio M. Persico, and Pat Levitt
  From the Cover: A genetic variant that disrupts MET transcription is associated with autism
  PNAS, Nov 2006; 103: 16834 - 16839.
  ... ...spontaneously aborted 22-week female fetus, was purchased from BioChain Institute, Inc. (Hayward, CA; catalog no. P2244035; lot...Human fetal brain nuclear protein was purchased from BioChain Institute, Inc. (Hayward, CA); the source of the human... ...
  Ref. link
  


• Daniel B. Campbell, James S. Sutcliffe, Philip J. Ebert, Roberto Militerni, Carmela Bravaccio, Simona Trillo, Maurizio Elia, Cindy Schneider, Raun Melmed, Roberto Sacco, Antonio M. Persico, and Pat Levitt
  A genetic variant that disrupts MET transcription is associated with autism
  PNAS, Oct 2006; 10.1073/pnas.0605296103.
  ... ...Human fetal brain nuclear protein was purchased from BioChain Institute, Inc. (Hayward, CA); the source of the human...spontaneously aborted 22-week female fetus, was purchased from BioChain Institute, Inc. (Hayward, CA; catalog no. P2244035; lot... ...
  Ref. link
  


• Raghavan Raju, Goran Rakocevic, Ziwei Chen, Gerard Hoehn, Cristina Semino-Mora, Wei Shi, Richard Olsen, and Marinos C. Dalakas
  Autoimmunity to GABAA-receptor-associated protein in stiff-person syndrome
  Brain, Sep 2006; 10.1093/brain/awl245.
  ... ...bleed was tested for specificity by enzyme-linked immunosorbent assay (ELISA) and in western blot using total human brain (Biochain, Hayward, CA). Immunoprecipitation and western blot Aliquots of 15 ml serum from SPS or OND control patients were incubated... ...
  Ref. link
  


• Mark R. Garbrecht, Thomas J. Schmidt, Zygmunt S. Krozowski, and Jeanne M. Snyder
  11-Hydroxysteroid dehydrogenase type 2 and the regulation of surfactant protein A by dexamethasone metabolites
  Am J Physiol Endocrinol Metab, Apr 2006; 290: E653 - E660.
  ... ...Loading was monitored by staining the blots with Ponceau S, a nonspecific protein dye. Homogenate proteins of human liver (BioChain, Hayward, CA) and Caco2 cells (human colon epithelial cell line, a gift of Dr. F. Jeffrey Field, University of Iowa) were... ...
  


• Xiaofeng Lu, Minhui Wang, Jin Qi, Haitao Wang, Xinna Li, Dipika Gupta, and Roman Dziarski
  Peptidoglycan Recognition Proteins Are a New Class of Human Bactericidal Proteins
  J. Biol. Chem., Mar 2006; 281: 5895 - 5907.
  ... ...esophagus, stomach, small intestine, and colon, from normal human donors (male and female, 21-65 years old), were prepared by BioChain. Following standard deparaffinization, re-hydration, and quenching of endogenous peroxidase by 30 min incubation in 0.3... ...

• Andrew M. F. Liu and Yung H. Wong
  Activation of Nuclear Factor B by Somatostatin Type 2 Receptor in Pancreatic Acinar AR42J Cells Involves G14 and Multiple Signaling Components: A MECHANISM REQUIRING PROTEIN KINASE C, CALMODULIN-DEPENDENT KINASE II, ERK, AND c-Src
  J. Biol. Chem., Oct 2005; 280: 34617 - 34625.
  ... ...luciferin substrate kit was a product of Roche Diagnostics. Normal rat pancreas membrane protein fraction was purchased from BioChain (Hayward, CA). Various antisera were products of Cell Signaling Technology (Beverly, MA) and Amersham Biosciences. Specific... ...
  


• Hamid Mehenni, Nathalie Lin-Marq, Karine Buchet-Poyau, Alexandre Reymond, Martine A. Collart, Didier Picard, and Stylianos E. Antonarakis
  LKB1 interacts with and phosphorylates PTEN: a functional link between two proteins involved in cancer predisposing syndromes
  Hum. Mol. Genet., Aug 2005; 14: 2209 - 2219.
  ... ...immunoprecipitate endogenous proteins with antibodies against PTEN, a commercial total protein extract from human small intestine (BioChain) was used. For immunoblotting, antibodies to LKB1 or to PTEN (both from Santa Cruz Biotechnology) were diluted 1:1000... ...
  
  
  
• Wei Li, Zu-Xi Yu, and Robert M. Kotin
  Profiles of PrKX Expression in Developmental Mouse Embryo and Human Tissues
  J. Histochem. Cytochem., Aug 2005; 53: 1003 - 1009.
  ... ...or fetal tissues including brain, heart, kidney, liver, lung, pancreas, spleen, and thymus were commercially obtained (BioChain Institute Hayward, CA). According to the manufacturer, the protein concentrations from each tissue were determined using... ...
  


• Thomas A. Hughes and Hugh J. M. Brady
  Expression of axin2 Is Regulated by the Alternative 5'-Untranslated Regions of Its mRNA
  J. Biol. Chem., Mar 2005; 280: 8581 - 8588.
  .... RNA and protein that had been purified simultaneously from single tissue samples was purchased from BioChain (AMS Biotechnology). cDNA samples were subjected to semi-quantitative PCR using RedTaq (Sigma) and the following ...
  


• Angela Relógio, Claudia Ben-Dov, Michael Baum, Matteo Ruggiu, Christine Gemund, Vladimir Benes, Robert B. Darnell, and Juan Valcárcel
  Alternative Splicing Microarrays Reveal Functional Expression of Neuron-specific Regulators in Hodgkin Lymphoma Cells
  Biol. Chem., Feb 2005; 280: 4779 - 4784.
  ... Lymph node tumor samples from lymphoma patients were purchased from BioChain (catalog number P1235161A-1, P1235161B-1). ...
  


• Reeves J, Xuan J, Arfanis K, Morin C, Garde S, Ruiz M, Wisniewski J, Panchal C, Tanner J.
  Identification, purification and characterization of a novel human blood protein with binding affinity for prostate secretory protein of 94 amino acids
  Biochem J. 2005 Jan 1; 385(Pt 1): 105-114.
  ... ...Total protein lysates from human prostate, pituitary and ovary were obtained from BioChain Institute (Hayward, CA, U.S.A.). All other reagents used were of analytical grade. ... ...
  Ref. link
  


• X. Chang, R. Yamada, A. Suzuki, T. Sawada, S. Yoshino, S. Tokuhiro, and K. Yamamoto
  Localization of peptidylarginine deiminase 4 (PADI4) and citrullinated protein in synovial tissue of rheumatoid arthritis
  Rheumatology, Jan 2005; 44: 40 - 50.
  ... The total protein in a commercial liver sample served as the control (Biochain). ...
  


• Friederike Fellenberg, Tanja B. Hartmann, Reinhard Dummer, Dirk Usener, Dirk Schadendorf, and Stefan Eichmüller
  GBP-5 Splicing Variants: New Guanylate-Binding Proteins with Tumor-Associated Expression and Antigenicity
  J. Invest. Dermatol., Jun 2004; 122: 1510 - 1517.
  ... cell lysate and commercial protein medleys of normal tissues (Clontech, Palo Alto, California, USA or BioChain, Hayward, California, USA). ...
  


• Miki Shimada, Reiko Terazawa, Yoshiteru Kamiyama, Wataru Honma, Kiyoshi Nagata, and Yasushi Yamazoe
  Unique properties of a renal sulfotransferase, St1d1 in dopamine metabolism
  J. Pharmacol. Exp. Ther., Apr 2004; 10.1124/jpet.104.065532.
  ... Human kidney cytosols were obtained from BioChain Institute, Inc. ...
  
  
• Stephen A. Renshaw, Clare E. Dempsey, Frances A. Barnes, Stephanie M. Bagstaff, Steven K. Dower, Colin D. Bingle, and Moira K. B. Whyte
  Three Novel Bid Proteins Generated by Alternative Splicing of the Human Bid Gene
  J. Biol. Chem., Jan 2004; 279: 2846 - 2855.
  ... Preprepared protein samples were from the BioChain Institute (Hayward, CA). ...
  


• Andrew M. Davidoff, Catherine Y. C. Ng, Junfang Zhou, Yunyu Spence, and Amit C. Nathwani
  Sex significantly influences transduction of murine liver by recombinant adeno-associated viral vectors through an androgen-dependent pathway
  Blood, Jul 2003; 102: 480 - 488.
  ... normal liver nuclear protein and Swiss Webster mouse hepatocyte nuclear extract were also purchased from BioChain Institute (Hayward, CA).
  


• Asako Otomo, Shinji Hadano, Takeya Okada, Hikaru Mizumura, Ryota Kunita, Hitoshi Nishijima, Junko Showguchi-Miyata, Yoshiko Yanagisawa, Eri Kohiki, Etsuko Suga, Masanori Yasuda, Hitoshi Osuga, Takeharu Nishimoto, Shuh Narumiya, and Joh-E Ikeda
  ALS2, a novel guanine nucleotide exchange factor for the small GTPase Rab5, is implicated in endosomal dynamics
  Hum. Mol. Genet., Jul 2003; 12: 1671 - 1687.
  ... Protein samples for the human cerebral cortex and cerebellum were purchased from Biochain Inc. ...
  


• Andrew M Davidoff, Catherine Y C Ng, Junfang Zhou, Yunyu Spence, and Amit C Nathwani
  Gender significantly influences transduction of murine liver by recombinant adeno-associated viral vectors through an androgen-dependent pathway
  Blood, Mar 2003; 200209288
  ... normal liver nuclear protein and Swiss Webster mouse hepatocyte nuclear extract were also purchased from (BioChain Institute Hayward, CA). ...
  ... and female mice, 2 weeks after castration or subcutaneous implantation of DHT pellets, respectively, by (BioChain Institute. ...)
  


• Yong Li, Kaihua Wei, Chengrong Lu, Yingxian Li, Ming Li, Guichun Xing, Handong Wei, Qingming Wang, Jizhong Chen, Chutse Wu, Huipeng Chen, Songcheng Yang, and Fuchu He
  Identification of hepatopoietin dimerization, its interacting regions and alternative splicing of its transcription
  Eur. J. Biochem., Aug 2002; 269: 3888 - 3893.
  ...nuclear extracts of three different human liver tissues (fetal liver, adult normal liver and hepatoma, Biochain) were resolved on SDS PAGE, transferred to poly(vinylidene difluoride) membrane, and analyzed by immunoblotting with ...


• Chengrong Lu, Yong Li, Yanlin Zhao, Guichun Xing, Fei Tang, Qingming Wang, Yuhui Sun, Handong Wei, Xiaoming Yang, Chutse Wu, Jianguo Chen, Kun-liang Guan, Chenggang Zhang, Huipeng Chen, and Fuchu He
  Intracrine hepatopoietin potentiates AP-1 activity through JAB1 independent of MAPK pathway
  FASEB J, Nov 2001; 105061. (Liver protein lysate from BioChain...)
  
  
• Katsuki Ohtani, Yasuhiko Suzuki, Souji Eda, Takao Kawai, Tetsuo Kase, Hiroyuki Keshi, Yoshinori Sakai, Atsushi Fukuoh, Takashi Sakamoto, Hiroyuki Itabe, Tatsuo Suzutani, Masahiro Ogasawara, Itsuro Yoshida, and Nobutaka Wakamiya
  The Membrane-type Collectin CL-P1 Is a Scavenger Receptor on Vascular Endothelial Cells
  J. Biol. Chem., Nov 2001; 276: 44222 - 44228. 
  ...Western blotting analyses were performed using CL-P1 transfected cells, HUVEC , placental tissue membrane extracts (BioChain Institute, Inc., CA) without or with de-glycosylation (Enzymatic Deglycosylation kit, Bio-Rad), and in vitro transcription ...
  


• Hiroshi Sakamoto, Tetsuo Mashima, Shigeo Sato, Yuichi Hashimoto, Takao Yamori, and Takashi Tsuruo
  Selective Activation of Apoptosis Program by S-p-bromobenzylglutathione Cyclopentyl Diester in Glyoxalase I-overexpressing Human Lung Cancer Cells
  Clin. Cancer Res., Aug 2001; 7: 2513 - 2518.
  ... Cytoplasmic protein from human normal tissue was purchased from BioChain Institute, Inc. ...
  ... normal tissues for GLO1 expressions using 48 Dot Human Tumor/Normal Tissue Total RNA Dot Blot (BioChain Institute, Inc.). ...
  


• Friederike Fellenberg, Tanja B. Hartmann, Reinhard Dummer, Dirk Usener, Dirk Schadendorf, and Stefan Eichmüller
  GBP-5 Splicing Variants: New Guanylate-Binding Proteins with Tumor-Associated Expression and Antigenicity
  J. Invest. Dermatol., Jun 2004; 122: 1510 - 1517.
  ... cell lysate and commercial protein medleys of normal tissues (Clontech, Palo Alto, California, USA or BioChain, Hayward, California, USA). ...