Promotions

Others:

• Christoph Coch, Nicolas Busch, Vera Wimmenauer, Evelyn Hartmann, Markus Janke, Mona Mohamed Ahmed Abdel-Mottaleb, Alf Lamprecht, Janos Ludwig, Winfried Barchet, Martin Schlee, and Gunther Hartmann
  Higher activation of TLR9 in plasmacytoid dendritic cells by microbial DNA compared with self-DNA based on CpG-specific recognition of phosphodiester DNA
  J. Leukoc. Biol., Jul 2009; 10.1189/jlb.0509314.
  ... ...chloroform-isopropanol (25/24/1) and ethanol precipitation (Esche-richia coli, leukocytes, HEK293 cells) or purchased from BioChain (Hay-ward, CA, USA; leukocytes) and Invivogen (San Diego, CA, USA; endotoxin-free E. coli DNA). Preparation, isolation, and... ...
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• Eva K. Wirth, Stephan Roth, Cristiane Blechschmidt, Sabine M. Hölter, Lore Becker, Ildiko Racz, Andreas Zimmer, Thomas Klopstock, Valerie Gailus-Durner, Helmut Fuchs, Wolfgang Wurst, Thomas Naumann, Anja Bräuer, Martin Hrab?de Angelis, Josef Köhrle, Annette Grüters, and Ulrich Schweizer
  Neuronal 3',3,5-Triiodothyronine (T₃) Uptake and Behavioral Phenotype of Mice Deficient in Mct8, the Neuronal T₃ Transporter Mutated in Allan–Herndon–Dudley Syndrome
  J. Neurosci., Jul 2009; 29: 9439 - 9449.
  ... ...preabsorbed quality. Human multiple tissue Western blot custom manufactured from crude membrane fractions was purchased from BioChain (BioCat). Western blot. Mouse organs were frozen on dry ice directly after dissection, powdered under liquid nitrogen... ...
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• Jason A. Dubovsky, Douglas G. McNeel, John J. Powers, John Gordon, Eduardo M. Sotomayor, and Javier A. Pinilla-Ibarz
  Treatment of Chronic Lymphocytic Leukemia with a Hypomethylating Agent Induces Expression of NXF2, an Immunogenic Cancer Testis Antigen
  Clin. Cancer Res., May 2009; 15: 3406 - 3415.
  ... ...from centrifugally pelleted cell cultures (RNeasy mini columns and RNAse free DNAse; Qiagen) or was commercially obtained (BioChain). RT-PCR reactions were conducted using the Qiagen one-step RT-PCR kit (Qiagen) with transcript-specific primers (Supplementary... ...
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• Elisavet A. Papageorgiou, Heike Fiegler, Vardhman Rakyan, Stephan Beck, Maj Hulten, Klea Lamnissou, Nigel P. Carter, and Philippos C. Patsalis
  Sites of Differential DNA Methylation between Placenta and Peripheral Blood: Molecular Markers for Noninvasive Prenatal Diagnosis of Aneuploidies
  Am. J. Pathol., May 2009; 174: 1609 - 1618.
  ... ...used in this study were obtained from AMS Biotechnology (Europe) Ltd. (Oxon, UK). These samples, originally sourced from the Biochain Institute (Hayward, CA), were subject to consent using ethical internal review board-approved protocols. In total, five female... ...
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• Queenie CK Cheung, Zongfei Yuan, Paul W Dyce, De Wu, Kees DeLange, and Julang Li
  Generation of epidermal growth factor–expressing Lactococcus lactis and its enhancement on intestinal development and growth of early-weaned mice
  Am. J. Clinical Nutrition, Mar 2009; 89: 871 - 879.
  ... ...streptavidin (DakoCytomation, Glostrup, Denmark) for 25 min and reacted with 1 mL 3,3-diamino-benzidine tetrahydrochloride (Biochain, CA) as the colored chromogen for visualization of the antigenic structures. Slides were then counterstained in Harris' hematoxylin... ...
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• Adeline Bertola, Vanessa Deveaux, Stéphanie Bonnafous, Déborah Rousseau, Rodolphe Anty, Abdelilah Wakkach, Moncef Dahman, Joan Tordjman, Karine Clément, Siobhán E. McQuaid, Keith N. Frayn, Pierre-Michel Huet, Jean Gugenheim, Sophie Lotersztajn, Yannick Le Marchand-Brustel, Albert Tran, and Philippe Gual
  Elevated Expression of Osteopontin May Be Related to Adipose Tissue Macrophage Accumulation and Liver Steatosis in Morbid Obesity
  Diabetes, Jan 2009; 58: 125 - 133.
  ... ...Clontech (Mountain View, CA) (male, 51 years old, sudden death), and Biochain (Hayward, CA) (four males: 24, 26, 28, and 30 years old, sudden deaths). The Stratagene and Biochain companies confirmed that histological findings were completely normal... ...
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• S¨¦bastien Lenglet, Fabrice Antigny, Laur¨¨ne Vetterli, Jean-François Dufour, and Michel F. Rossier
  Hint2 is expressed in the mitochondria of H295R cells and is involved in steroidogenesis
  Endocrinology, Jul 2008; 10.1210/en.2008-0400.
  ... ...immunobloting. Hint2 immunoblot analysis Submitochondrial distribution of Hint2 was analyzed on a commercially available membrane (Biochain, Lugano, Switzerland). Briefly, fractionated mitochondrial membranes were loaded on a 4-20% denaturating polyacrylamide gel... ...
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• Xudong Liao, Wei Wang, Shenghan Chen, and Qingyu Wu
  Role of Glycosylation in Corin Zymogen Activation
  J. Biol. Chem., Sep 2007; 282: 27728 - 27735.
  ... ...A human corin cDNA fragment containing the entire open reading frame was amplified by PCR from a human fetal heart library (BioChain, Hayward, CA) using Phusion High Fidelity polymerase with sense primer 5-AGA GAA AAG CGA CCA AGA TAA A-3 and antisense primer... ...
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• Daisuke Shiokawa, Tokiyoshi Matsushita, Yukari Shika, Mamoru Shimizu, Masahiro Maeda, and Sei-ichi Tanuma
  DNase X is a GPI-anchored membrane enzyme that provides a barrier to endocytosis-mediated transfer of a foreign gene
  J. Biol. Chem., Apr 2007; 10.1074/jbc.M610428200.
  ... ...paraffin-embedded normal human tissues were obtained from BioChain. Immunohistochemistry was performed using a Cell and Tissue...slides, pre-treated with an Antigen Retrieval Dewax Solution (BioChain), were incubated with anti-DNase X mAb at a concentration of... ...
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• Sharma M, Li X, Wang Y, Zarnegar M, Huang CY, Palvimo JJ, Lim B, Sun Z.
  hZimp10 is an androgen receptor co-activator and forms a complex with SUMO-1 at replication foci
  EMBO J. 2003 Nov 17; 22(22): 6101-6114.
  ... ...The C-terminus of hZimp10 (aa 917?067) was inserted into the pGEX-4T1 vector (Amersham). The GST fusion protein was prepared as described previously (Smith and Johnson, 1988). The GST–hZimp10 fusion protein was used as a source of antigen for antibody production. The antibody was produced and purified by BioChain Institute Inc. (Hayward, CA). ... ...
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• Adeline M. Hajjar, D. Shane O¡¯Mahony, Adrian Ozinsky, David M. Underhill, Alan Aderem, Seymour J. Klebanoff, and Christopher B. Wilson
  Cutting Edge: Functional Interactions Between Toll-Like Receptor (TLR) 2 and TLR1 or TLR6 in Response to Phenol-Soluble Modulin
  J. Immunol., Jan 2001; 166: 15 - 19. ... Cloning of murine TLR1 Total RAW 264.7 cell RNA was isolated using RNA exol (BioChain Institute, San Leandro, CA). ...
  


• Manal A. Morsy, MingCheng Gu, Sherri Motzel, Jing Zhao, Jing Lin, Qin Su, Henry Allen, Laura Franlin, Robin J. Parks, Frank L. Graham, Stefan Kochanek, Andrew J. Bett, and C. Thomas Caskey
  An adenoviral vector deleted for all viral coding sequences results in enhanced safety and extended expression of a leptin transgene
  PNAS, Jul 1998; 95: 7866 - 7871. A probe for {beta}-actin was used as the internal control (≈1 kb) (Biochain, San Leandro, CA). ...
  
  
• Huan-You Wang, Wen Lin, Jacqueline A. Dyck, Joanne M. Yeakley, Zhou Songyang, Lewis C. Cantley, and Xiang-Dong Fu
  SRPK2: A Differentially Expressed SR Protein-specific Kinase Involved in Mediating the Interaction and Localization of Pre-mRNA Splicing Factors in Mammalian Cells
  J. Cell Biol., Feb 1998; 140: 737 - 750. ... Total RNA from 6 * 10 5 cultured cells was extracted using RNAexol (BioChain, San Leandro, CA); 15-20 µg of total RNA was separated in 1% agarose gel containing ...