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Northern Blots:
-
Sachie Nakamichi, Yoko Senga, Hiroshi Inoue, Aki Emi,
Yasushi Matsuki, Eijiro Watanabe, Ryuji Hiramatsu, Wataru
Ogawa, and Masato Kasuga
Role of the E3 ubiquitin ligase gene related to
anergy in lymphocytes in glucose and lipid metabolism in the
liver
 J.
Mol. Endocrinol., Feb 2009; 42: 161 - 169.
 ...
...analysis, a membrane loaded with 20mug total RNA
extracted from various mouse organs (Mouse Tissue Total RNA
Northern Blot; BioChain,
Hayward, CA, USA) was probed with a 32P-labeled fragment of
mouse GRAIL cDNA (nucleotides 138-482) isolated by PCR. For
assay... ...
Ref. link
-
Damien Carrel, Justine Masson, Sana Al Awabdh, Catherine
Borg Capra, Zsolt Lenkei, Michel Hamon, Michel Boris Emerit,
and Mich¨¨le Darmon
Targeting of the 5-HT1A Serotonin
Receptor to Neuronal Dendrites Is Mediated by Yif1B
J.
Neurosci., Aug 2008; 28: 8063 - 8073.
...
...interactions (Wojcik et al., 2002). Northern blot
experiments. The mRNA Northern blot NBA (Normalized By
Amount of RNA; BioChain Institute)
was hybridized using [alpha-32P]dCTP (GE Healthcare) labeled
probe made following the Rediprime II Random Prime... ...
Ref. link
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Ravinder K. Gill, Nitika Pant, Seema Saksena, Amika Singla, Talat M. Nazir, Lisa Vohwinkel, Jerrold R. Turner, Jay Goldstein, Waddah A. Alrefai, and Pradeep K. Dudeja
Function, expression, and characterization of the serotonin transporter in the native human intestine
Am J Physiol Gastrointest Liver Physiol, Jan 2008; 294: G254 - G262.
... ...colon were commercially obtained (from BioChain). Human SERT was amplified with gene...human multiple-tissue Northern blots (BioChain) each containing poly(A) RNA (3 ug/lane...human multiple-tissue Northern blots (BioChain) were probed with 32P-labeled 491-bp... ...
Ref. link
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Sumit Agarwal, Josephine Harada, Jeffrey Schreifels, Patrycja Lech, Bryan Nikolai, Tomoyuki Yamaguchi, Sumit K. Chanda, and Nikunj V. Somia
Isolation, characterization, and genetic complementation of a cellular mutant resistant to retroviral infection
PNAS, Oct 2006; 103: 15933 - 15938.
... ...Blot Analysis. Northern blot analysis was performed as described previously (3). A human RNA tissue blot was purchased from BioChain Institute (Hayward, CA). Cellular Localization. Immunocytochemistry was carried out essentially as described previously (3... ...
Ref. link
- D.
Monk, R. Sanches, P. Arnaud, S. Apostolidou, F.A. Hills,
S. Abu-Amero, A. Murrell, H. Friess, W. Reik, P. Stanier,
M. Constância, and G.E. Moore
Imprinting of IGF2 P0 transcript and novel alternatively
spliced INS-IGF2 isoforms show differences between mouse
and human
Hum.
Mol. Genet., Apr 2006; 15: 1259 - 1269.
...
...containing polyARNA (2microg per lane) was purchased
from Ambion. Custom-made fetal northern blots were obtained
from BioChain. The IGF2
RNA probes, which encompassed the P1-specific promoter region
(130501-130800 of AC0130303), P0-specific promoter... ...
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Sass JO, Mohr V, Olbrich H, Engelke U, Horvath J, Fliegauf M, Loges NT, Schweitzer-Krantz S, Moebus R, Weiler P, Kispert A, Superti-Furga A, Wevers RA, Omran H.
Mutations in ACY1, the Gene Encoding Aminoacylase 1, Cause a Novel Inborn Error of Metabolism
Am J Hum Genet. 2006 Mar; 78(3): 401-409.
... ...Tissue specificity of expression of the human ACY1 gene was analyzed by northern blot hybridization, by use of a 657-bp BsmI/ScaI fragment of the human cDNA (IMAGp958G107Q [RZPD Web site]) as a probe. Human multiple-tissue northern blots (BioChain Institute and BD Biosciences) were hybridized overnight at 63.5°C in Church buffer .... ...
Ref. link
- Ning
Qin, Sui-Po Zhang, Tasha L. Reitz, Jay M. Mei, and Christopher
M. Flores
Cloning, Expression, and Functional Characterization
of Human Cyclooxygenase-1 Splicing Variants: Evidence for
Intron 1 Retention
J.
Pharmacol. Exp. Ther., Dec 2005; 315: 1298 - 1305.
...
...Different Types of Tumor mRNA blots were purchased from
BioChain Institute,
Inc. (Hayward, CA). Each blot was prehybridized...multiple
tissue total protein blots were purchased from BioChain
Institute, Inc. Blots were blocked with 5% dry milk in...
...
- Sadaharu
Masuyama, Satoshi Tateishi, Kentaro Yomogida, Yoshitake
Nishimune, Keiichiro Suzuki, Yoshiyuki Sakuraba, Hirokazu
Inoue, Michio Ogawa, and Masaru Yamaizumi
Regulated expression and dynamic changes in subnuclear
localization of mammalian Rad18 under normal and genotoxic
conditions
Genes
Cells, Aug 2005; 10: 753 - 762.
...
...hybridized with 32P-labeled human RAD18 cDNA using Multiple
Tissue Northern Blot (Clontech) and Total RNA Northern Blot
(BioChain). Chinese
hamster total RNA from various organs was hybridized with
32P-labeled mouse RAD18 cDNA. Northern blot analysis...
...
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Olsen CL, Hsu PP, Glienke J, Rubanyi GM, Brooks AR.
Mutations in the JARID1C Gene, Which Is Involved in Transcriptional Regulation and Chromatin Remodeling, Cause X-Linked Mental Retardation
Am J Hum Genet. 2005 Feb; 76(2): 227-236.
... ...Total RNA was isolated from patient and control lymphoblastoid cell lines by use of TRIzol (Invitrogen), in accordance with the manufacturer's protocol. Poly-A+ RNAs, which were each obtained from 100 µg of total RNA by use of Dynabeads (Dynal), were separated in a formaldehyde-containing gel in 1 ?MOPS buffer, transferred to a Hybond N+ membrane, and crosslinked by UV. A human multiple-tissue northern blot was obtained from BioChain.
... ...
Ref. link
- C.
van der Meer-van Kraaij, E. Kramer, D. Jonker-Termont, M.B.
Katan, R. van der Meer, and J. Keijer
Differential gene expression in rat colon by dietary
heme and calcium
Carcinogenesis, Jan 2005; 26: 73 - 79.
...
Northern blot analysis Northern blot analysis was performed
using pre-made
rat tissue blots (BioChain,
Clontech) with 2 or 3 {micro}g poly(A) + RNA, normalized
by expression of the {beta}-actin ...
- Sabine Meurer, Sylke Pioch,
Kristina Wagner, Werner Müller-Esterl, and Steffen Gross
AGAP1, a Novel Binding Partner of Nitric Oxide-sensitive
Guanylyl Cyclase
Biol. Chem., Nov 2004; 279: 49346 - 49354.
...
from BD Biosciences, and mouse tissue NBA (normalized by
amount of mRNA) blot came from BioChain
(Hayward, CA). ...
- Cynthia
Shannon Weickert, Richard E. Straub, Benjamin W. McClintock,
Mitsuyuki Matsumoto, Ryota Hashimoto, Thomas M. Hyde, Mary
M. Herman, Daniel R. Weinberger, and Joel E. Kleinman
Human Dysbindin (DTNBP1) Gene Expression
in Normal Brain and in Schizophrenic Prefrontal Cortex and
Midbrain
Arch
Gen Psychiatry, Jun 2004; 61: 544 - 555.
... N3234410; BioChain
Institute Inc, Hayward, Calif), containing total RNA from
adults (frontal lobe, temporal lobe, parietal lobe, ...
- John
F. Lockwood, Jingsong Cao, Paul Burn,
and Yuguang Shi
A Human Intestinal Monoacylglycerol Acyltransferase:
Differential Features in Tissue Expression and Activity
Am J Physiol Endocrinol Metab, Jun 2003; 10.1152/ajpendo.00179.2003.
... Biosciences Clontech, Palo Alto, CA and OriGene Technologies
Inc., Rockville, MD) and total RNA blots (Biochain
Institute, Inc., Hayward, CA) were hybridized with [ 32P]-dCTP
(ICN Radiochemicals) labeled probes prepared from ...
- Jingsong Cao, John Lockwood,
Paul Burn, and Yuguang Shi
Cloning and Functional Characterization of a Mouse Intestinal
Acyl-CoA:Monoacylglycerol Acyltransferase, MGAT2
J.
Biol. Chem., Apr 2003; 278: 13860 - 1386
...
(Rockville, MD) or BioChain
Institute Inc. ...
...
Mouse multiple tissue blots from Origene ( A ) and Biochain
( B ) containing 2 µg of poly(A) + RNA were hybridized with
radiolabeled probes ...
- Songzhu An, Gene Cutler,
Jack Jiagang Zhao, Shu-Gui Huang, Hui Tian, Wanbo Li, Lingming
Liang, Miki Rich, Amy Bakleh, Juan Du, Jin-Long Chen, and
Kang Dai
Identification and characterization of a melanin-concentrating
hormone receptor
PNAS,
Jun 2001; 98: 7576 - 7581.
... The human brain RNA blots were purchased from the Biochain
Institute. ...
...
Each lane of the Biochain
blots contains 20 µg of total RNA isolated from various
regions of the human brain. ...
- Makiko Meguro, Kohzoh Mitsuya,
Nobuo Nomura, Masakazu Kohda, Akiko Kashiwagi, Ryuichi Nishigaki,
Hirotaka Yoshioka, Mitsuyoshi Nakao, Michio Oishi, and Mitsuo
Oshimura
Large-scale evaluation of imprinting status in the Prader¨CWilli
syndrome region: an imprinted direct repeat cluster resembling
small nucleolar RNA genes
Hum.
Mol. Genet., Feb 2001; 10: 383 - 394.
... with [{alpha}- 32 P]dCTP by random priming and hybridized
to a Gene Hunter mRNA blot (BioChain).
...
- Teresa L. Born, Dirk E.
Smith, Kirsten E. Garka, Blair R. Renshaw, Jeanette S. Bertles,
and John E. Sims
Identification and Characterization of Two Members of
a Novel Class of the Interleukin-1 Receptor (IL-1R) Family.
DELINEATION OF A NEW CLASS OF IL-1R-RELATED PROTEINS BASED
ON SIGNALING
J.
Biol. Chem., Sep 2000; 275: 29946 - 29954. ... Normal and
cancer cell line human multiple tissue Northern blots were
purchased from CLONTECH and Biochain
Institute, Inc. ...
...
Northern blots containing mRNA from the indicated tissues
were purchased from CLONTECH Laboratories, Inc. or Biochain
Institute, Inc. ...
- Kimberly Kelly, Elizabeth
Manos, Gregory Jensen, Lincoln Nadauld, and David A. Jones
APRIL/TRDL-1, a Tumor Necrosis Factor-like Ligand, Stimulates
Cell Death
Cancer
Res., Feb 2000; 60: 1021 - 1027.
... A multiple tumor mRNA blot was obtained from
Biochain Institute,
Inc. ...
- Henning Walczak, Mariapia
A. Degli-Esposti, Richard S. Johnson, Pam J. Smolak,
Jennifer Y. Waugh, Norman Boiani, Martin S.
Timour, Mary J. Gerhart, Kenneth A. Schooley,
Craig A. Smith, Raymond G. Goodwin, and Charles
T. Rauch
TRAIL-R2: a novel apoptosis-mediating receptor for TRAIL
EMBO
J., Sep 1997; 16: 5386 - 5397.
... library screen
was added to two different human multiple-tissue Northern
blots (Clontech, Palo Alto, CA; Biochain,
Palo Alto, CA). ...
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