Promotions

Antibody:

• Chang Whan Lee, Tae Hoon Kim, Heung Man Lee, Seung Hoon Lee, Sung Ho Lee, Joon Hyuk Yoo, Yeon Soo Kim, and Sang Hag Lee
  Upregulation of Elafin and Cystatin C in the Ethmoid Sinus Mucosa of Patients With Chronic Sinusitis
  Arch Otolaryngol Head Neck Surg, Aug 2009; 135: 771 - 775.
  ... ...anti-elafin polyclonal antibody (Santa Cruz Biotechnology, Santa Cruz, California) or rabbit anti-cystatin C polyclonal antibody (BioChain Institute Inc, Hayward, California). The color was developed using 3, 3-diaminobenzidine. For Western blot analysis, equal... ...
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• David E. Ott, Lori V. Coren, and Teresa Shatzer
  The Nucleocapsid Region of Human Immunodeficiency Virus Type 1 Gag Assists in the Coordination of Assembly and Gag Processing: Role for RNA-Gag Binding in the Early Stages of Assembly
  J. Virol., Aug 2009; 83: 7718 - 7727.
  ... ...Perkin Elmer Life Sciences, Inc. Proteins were detected by developing blots with horseradish peroxidase-conjugated anti-goat (Biochain Institute, Hayward, CA) or anti-mouse (Millipore Corp., Telemuca, CA) secondary antibody and an Immun-star horseradish peroxidase... ...
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• Rachael M. Crist, Siddhartha A. K. Datta, Andrew G. Stephen, Ferri Soheilian, Jane Mirro, Robert J. Fisher, Kunio Nagashima, and Alan Rein
  Assembly Properties of Human Immunodeficiency Virus Type 1 Gag-Leucine Zipper Chimeras: Implications for Retrovirus Assembly
  J. Virol., Mar 2009; 83: 2216 - 2225.
  ... ...Ott, AIDS Vaccine Program, SAIC-Frederick). The secondary antibody was a rabbit anti-goat-horseradish peroxidase conjugate (BioChain Institute, Inc.). Both antibodies were used at a dilution of 1:10,000. Isolation of RNA from VLPs. RNA was extracted from... ... 
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• Lori M. Roberts, Kathleen Woodford, Mei Zhou, Deborah S. Black, Jill E. Haggerty, Emily H. Tate, Kent K. Grindstaff, Wondwessen Mengesha, Chandrasekaran Raman, and Noa Zerangue
  Expression of the Thyroid Hormone Transporters Monocarboxylate Transporter-8 (SLC16A2) and Organic Ion Transporter-14 (SLCO1C1) at the Blood-Brain Barrier
  Endocrinology, Dec 2008; 149: 6251 - 6261.
  ... ...fetal parietal lobe (female, 37 wk), hippocampus (female, 82 yr), and choroid plexus (female, 70 yr) were purchased from BioChain Institute (Hayward, CA). qPCR RNeasy RNA Isolation Kit and QIAshredder homogenizer columns (QIAGEN, Valencia, CA) were... ...
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• Luca Paoluzzi, Mithat Gonen, Govind Bhagat, Richard R. Furman, Jeffrey R. Gardner, Luigi Scotto, Volodia D. Gueorguiev, Mark L. Heaney, Katia Manova, and Owen A. O'Connor
  The BH3-only mimetic ABT-737 synergizes the antineoplastic activity of proteasome inhibitors in lymphoid malignancies
  Blood, Oct 2008; 112: 2906 - 2916.
  ... ...polyclonal antibodies were used: Bax (N20; Santa Cruz Biotechnology, Santa Cruz, CA), Bak (NT; GeneTex, San Antonio, TX), Puma (Biochain Institute, Hayward, CA), Noxa (Calbiochem, San Diego, CA), Mcl-1 (Rockland, Gilbertsville, PA), Bcl-2 (clone 100; Upstate... ...
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• Dietrich B. Conze, Chuan-Jin Wu, James A. Thomas, Allison Landstrom, and Jonathan D. Ashwell
  Lys63-Linked Polyubiquitination of IRAK-1 Is Required for Interleukin-1 Receptor- and Toll-Like Receptor-Mediated NF-B Activation
  Mol. Cell. Biol., May 2008; 28: 3538 - 3547.
  ... ...antibody (FL-419) were purchased from Santa Cruz Biotechnology. The anti-TRAF6 (CT) polyclonal antibody was purchased from Biochain Institute. The anti-NEMO MAb (C73-764) was purchased from BD Pharmingen. The anti-phospho-p38 polyclonal antibody (Thr 180... ...
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• T. E. Weber, C. J. Ziemer, and B. J. Kerr
  Effects of adding fibrous feedstuffs to the diet of young pigs on growth performance, intestinal cytokines, and circulating acute-phase proteins
  J Anim Sci, Apr 2008; 86: 871 - 881.
  ... ...15 min at 37C. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was detected using a mouse anti-GAPDH monoclonal antibody (BioChain Institute Inc., Hayward, CA) diluted at 1:2,000 in blocking solution. The GAPDH reaction complexes were visualized as described... ...
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• Peter I. Lobo, Kailo H. Schlegel, Clinton E. Spencer, Mark D. Okusa, Christopher Chisholm, Nino Mchedlishvili, Andrew Park, Constance Christ, and Christopher Burtner
  Naturally Occurring IgM Anti-Leukocyte Autoantibodies (IgM-ALA) Inhibit T Cell Activation and Chemotaxis
  J. Immunol., Feb 2008; 180: 1780 - 1791.
  ... ...Abs in the Western blot procedure: polyclonal rabbit IgG Abs to CD3 (Santa Cruz Biotechnology), CD4 (RD Systems), or CXCR4 (Biochain); monoclonal mouse IgG Abs to CCR5 (clone CTC, N-terminal) and CXCR4 (clone 4G10 N-terminal). Immunoprecipitation technique... ...
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• Peter I. Lobo, Kailo H. Schlegel, Wen Yuan, Gregory C. Townsend, and Jennifer A. White
  Inhibition of HIV-1 Infectivity through an Innate Mechanism Involving Naturally Occurring IgM Anti-Leukocyte Autoantibodies
  J. Immunol., Feb 2008; 180: 1769 - 1779.
  ... ...in the Western blot procedure: polyclonal rabbit IgG Abs to CD3 (Santa Cruz Biotechnology, CA), CD4 (RD Systems), or CXCR4 (Biochain); monoclonal mouse IgG Abs to CCR5 (clone CTC, N-terminal) and CXCR4 (clone 4G10 N-terminal). HIV-1 viral isolates R5 isolates... ...
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• Lori V. Coren, James A. Thomas, Elena Chertova, Raymond C. Sowder, II, Tracy D. Gagliardi, Robert J. Gorelick, and David E. Ott
  Mutational Analysis of the C-Terminal Gag Cleavage Sites in Human Immunodeficiency Virus Type 1
  J. Virol., Sep 2007; 81: 10047 - 10054.
  ... ...Proteins were detected by developing blots with either horseradish peroxidase-conjugated anti-goat, anti-rabbit (both from Biochain Institute, Hayward, CA), or anti-mouse (Life Technologies, Inc., Gaithersburg, MD) secondary antibody and the Immun-star horseradish... ...
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• EYTAN R. BARNEA
  Applying Embryo-Derived Immune Tolerance to the Treatment of Immune Disorders
  Ann. N.Y. Acad. Sci., Sep 2007; 1110: 602 - 618.
  ... ...USA). The antibodies were used to stain fetal tissues (14-18 weeks) placed on two microarrays (60 tissues each) obtained from BioChain Inc. (Hayward, CA, USA). Immunohistochemistry Staining of the slides was carried out using standard techniques. Slides... ...
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• Lori V. Coren, James A. Thomas, Elena Chertova, Raymond C. Sowder, II, Tracy D. Gagliardi, Robert J. Gorelick, and David E. Ott
  Mutational Analysis of the C-Terminal Gag Cleavage Sites in Human Immunodeficiency Virus Type 1
  J. Virol., Jul 2007; 10.1128/JVI.02496-06.
  ... ...Wellesley, MA). Proteins were detected by developing blots with either HRP-conjugated12 anti-goat, anti-rabbit (both from Biochain Institute, Hayward, CA), or anti-mouse (Life13 Technologies, Inc., Gaithersburg, MD) secondary antibody and the Immun-star... ...
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• Samuel J. Rulli, Jr., Catherine S. Hibbert, Jane Mirro, Thoru Pederson, Shyam Biswal, and Alan Rein
  Selective and Nonselective Packaging of Cellular RNAs in Retrovirus Particles
  J. Virol., Jun 2007; 81: 6623 - 6631.
  ... ...Ott, AIDS Vaccine Program, SAIC-Frederick), followed by the appropriate horseradish peroxidase-labeled secondary antibody (BioChain Institute, Inc.). The protein of interest was quantitated using Supersignal West Dura extended-duration substrate (Pierce... ...
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• Samuel J. Rulli, Jr., Catherine S. Hibbert, Jane Mirro, Thoru Pederson, Shyam Biswal, and Alan Rein
  SELECTIVE AND NON-SELECTIVE PACKAGING OF CELLULAR RNAS IN RETROVIRUS PARTICLES
  J. Virol., Mar 2007; 10.1128/JVI.02833-06.
  ... ...Vaccine Program, SAIC-Frederick) followed by176 ACCEPTED 9 the appropriate horseradish peroxidase-labeled secondary antibody (BioChain Institute,177 Inc.). The protein of interest was quantitated using Supersignal West Dura extended178 duration substrate (Pierce... ...
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• Li XL, Shuai JB, Fang WH.
  Protection of Carassius auratus Gibelio against infection by Aeromonas hydrophila using specific immunoglobulins from hen egg yolk
  J Zhejiang Univ Sci B. 2006 Nov; 7(11): 922-928. published online before print October 17, 2006
  ... ...One hundred microlitres of HRP conjugated rabbit anti-chicken IgG (BioChain Institute, Inc., Hayward, USA) at 500-fold dilution with PBST with 0.05 BSA was added to the wells after another three washings.... ...
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• David E. Ott, Lori V. Coren, and Tracy D. Gagliardi
  Redundant Roles for Nucleocapsid and Matrix RNA-Binding Sequences in Human Immunodeficiency Virus Type 1 Assembly
  J. Virol., Nov 2005; 79: 13839 - 13847.
  ... ...Proteins were detected by developing blots with a horseradish peroxidase (HRP)-conjugated anti-goat secondary antibody (BioChain Institute, Hayward, CA) and the Immun-star HRP substrate kit (Bio-Rad, Hercules, CA) on LumiFilm (Roche Applied Science... ...
  


• David E. Ott, Lori V. Coren, Tracy D. Gagliardi, and Kunio Nagashima
  Heterologous Late-Domain Sequences Have Various Abilities To Promote Budding of Human Immunodeficiency Virus Type 1
  J. Virol., Jul 2005; 79: 9038 - 9045.
  ... ...Frederick, MD). Proteins were detected by development with horseradish peroxidase-conjugated anti-goat secondary antibody (BioChain Institute, Hayward CA) and the Immun-star horseradish peroxidase substrate kit (Bio-Rad, Hercules, CA) on LumiFilm, Roche... ...
  
  
• Ott DE, Coren LV, Gagliardi TD, Nagashima K.
  Heterologous Late-Domain Sequences Have Various Abilities To Promote Budding of Human Immunodeficiency Virus Type 1
  J Virol. 2005 Jul; 79(14): 9038-9045.
  ... ...Proteins were detected by development with horseradish peroxidase-conjugated anti-goat secondary antibody (Biochain Institute, Hayward CA) and the Immun-star horseradish peroxidase substrate kit ... ...
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• MEMBRANE TRANSPORT STRUCTURE FUNCTION AND BIOGENESIS:
  Antony S. Dimitrov, Xiaodong Xiao, Dimiter S. Dimitrov, and Robert Blumenthal
  Early Intermediates in HIV-1 Envelope Glycoprotein-mediated Fusion Triggered by CD4 and Co-receptor Complexes
  J. Biol. Chem., Aug 2001; 276: 30335 - 30341. ... performed using the T4¨C4 anti CD4 antibody (NIH AIDS R&RRP), a rabbit polyclonal anti-CXCR4 antibody (Biochain Institute, Hayward, CA), and a goat polyclonal anti-CCR5 antibody (Santa Cruz Biotechnology Inc., Santa Cruz, ...