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microRNA Detection
Designed for use with BioChain¡¯s MicroRNA qRT-PCR
primer sets, the MicroRNA One-Step qRT-PCR Detection
Kit provides a one-step, simple, robust, inexpensive
assay for quantitative analysis of miRNA expression
from total RNA samples, or small RNAs enriched
samples. In this kit, EvaGreen dye, a superior green
fluorescence DNA-binding dye is used for real-time
detection and quantification of DNA. This kit
contains an enzyme mix, a 2x- concentrated qRT-PCR
reaction mixture and a miRNA primer set that contain
all the reagents (except templates) needed for
running qRT-PCR assay. The kit includes the
hsa-miR-24 (Cat# KS081200) or hsa-miR-16 (Cat#
KS081200) qRT-PCR primer set for amplification of
the widely expressed miR-24 or miR-16 from either
the Human Placenta Total RNA supplied with the kit
or from user-supplied human, mouse and rat RNA
samples.
Features
¡ñ
Convenient - All reaction components are supplied
for quick and easy set up
¡ñ
Save time
- One-step qRT-PCR procedure reduces setup
time and liquid handling steps
¡ñ
Wide dynamic range: good linearity and excellent PCR
efficiency over a 6 logs of dynamic range
¡ñ
High Sensitivity - detect miRNA expression in as low
as 2.5pg total RNA
¡ñ
Flexible
- Compatible with most of the real-time PCR
instruments
Application
¡ñ
MicroRNA detection and quantification
¡ñ
MicroRNA expression profiling
¡ñ
MicroRNA array validation
Quality
Control
1 kit of this lot has been tested for amplifying
plasmid containing human β-actin cDNA (amplified
fragment: 202 bp) over an 8 orders of dynamic range
using Stratagene¡¯s Mx3005P as a real time PCR
instrument. Good linearity and great PCR efficiency
is observed and consistent with the previous lot. |
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