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Universal cDNA
Features
 ● Made from very diversified tissues for broad gene coverage
● Industrial production scale to minimize lot-to-lot variation
● High quality without genomic DNA contamination

Application
● Used as reference for any kind of array study

 Quality Controls
 ● The integrity of the RNA used for cDNA synthesis is examined by visual inspection for the presence of intact bands of 18s and 28s ribosomal RNA when electrophoreses on a denaturing agarose gel. The quality and purity of total RNA were tested by spectrophotometer. A260/280 is between 1.8 and 2.0 (detected in 10 mM Tris-Cl, pH 7.5). The ratio of 28S/18S is ≥1.
● The RNA used for cDNA synthesis is treated by DNase I, and is tested as DNA free RNA by PCR.
● The Universal cDNA was selected to ensure its full length. The cDNA was used as template for PCR amplification of β-actin gene and an 838 bp β-actin band was visualized on 2% agarose gel. β-actin control primer is included. It is enough for 10 PCR reactions.
   Chicken (Normal)  new
   Dog (Normal)  new
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   Human (Normal)
   Mouse (Normal)
   Rat (Normal)

 

 

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BioChain Institute, Inc. - 3517 Breakwater Avenue, Hayward, CA 94545, USA
- Tel: 888.762.2568 or 510.783.8588 - fax: 510.783.5386
e-mail: order@biochain.com website: www.biochain.com
All of our products are intended to be used for RESEARCH purposes only.
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