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Universal RNA
Features
● Made from very diversified tissues for broad gene coverage
● Industrial production scale to minimize lot-to-lot variation
● High quality without genomic DNA contamination

Application
● Used as reference for any kind of array study

Quality Controls
● The integrity of the RNA is examined by visual inspection for the presence of intact
bands of 18s and 28s ribosomal RNA when electrophoreses on a denaturing agarose
gel. The quality and purity of total RNA were tested by spectrophotometer. A260/280 is
between 1.8 and 2.0 (detected in 10mM Tris-HCl, pH 7.5)
● The Universal RNA is analyzed by capillary electrophoresis with Agilent 2100
bioanalyzer. RNA integrity is determined with RIN (RNA Integrity Number)≥7, and the
area of 28s over 18s ratio ≥1.
● The RNA is treated by DNase I, and is tested as DNA free RNA by PCR
● cDNA synthesis is successfully performed by using this RNA as template
● Universal RNA is labeled and hybridized to a cDNA microarray, expected result of
gene expression is consistent with each lot.
   Chicken (Normal)  new
   Dog (Normal)  new
   Monkey (Normal)  new
   Human (Normal)
   Mouse (Normal)
   Rat (Normal)

 

 

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BioChain Institute, Inc. - 3517 Breakwater Avenue, Hayward, CA 94545, USA
- Tel: 888.762.2568 or 510.783.8588 - fax: 510.783.5386
e-mail: order@biochain.com website: www.biochain.com
All of our products are intended to be used for RESEARCH purposes only.
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