Total RNA
Features
¡ñ Total RNA isolated
from a wide variety of hard to obtain tissues
¡ñ Decontamination of polysaccharide, proteoglycan,
RNase, and genomic DNA
¡ñ Extensive quality control procedures to ensure
high quality
¡ñ High efficiency reverse transcription
¡ñ Available documentation of tissues' clinical
histories
Application
¡ñ Northern Blotting, RT-PCR, and RACE
¡ñ cDNA synthesis and cDNA library construction
¡ñ cDNA probe for profiling study in gene expression
¡ñ RNA protection and primer extension
¡ñ RNA display
¡ñ Purification of mRNA
Quality Controls
¡ñ The integrity of the
RNA is examined by visual inspection for the
presence of intact bands of 18s and 28s ribosomal
RNA when electrophoreses on a denaturing agarose
gel. The quality and purity of total RNA were tested
by spectrophotometer. A260/280 is between 1.8 and
2.0 (detected in 10 mM Tris-HCl, pH 7.5).
¡ñ The RNA is treated by DNAse I, and is tested as
DNA free RNA by PCR.
¡ñ cDNA synthesis is successfully performed by using
this RNA as template. |
