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Small Size
Genomic DNA
PCR-based methods for the analysis of genomic DNA are
becoming increasingly common both in research and for
routine purposes. Genomic DNA on an average are between 50 -
150 kb, when the large molecule genomic DNA is used as PCR
template, it might not be distributed evenly in solution
when a master mix need to be made for multiple PCR
reactions. High Throughput Digital PCR is a newly developed
technology for high throughput real time PCR reaction,
making master mix is inevitable in this technology. It is
very crucial to make every molecule evenly distributed into
each capilary chamber on the dynamic arrays. Large size
genomic DNA molecules will result in uneven distribution
while smaller size genomic will be even and randomly
distributed. BioChain's small size genomic DNA will help you
perform digital PCR successfully.
Features
¡ñ Genomic DNA isolated
from a wide variety of hard to obtain tissues
¡ñ
Decontamination of polysaccharide and proteoglycan
¡ñ Extensive quality control procedures ensure high quality
genomic DNA
¡ñ High efficiency in PCR
¡ñ Documentation of tissues' clinical histories available
Applications
¡ñ Control template for
digital PCR
¡ñ SNP analysis and PCR
¡ñ Profiling study in gene expression
Quality Controls
¡ñ The quality and
purity of genomic DNA were tested by spectrophotometer and
electrophoresis.
A260/280 is between 1.8 and 2.0 (detected in 10 mM Tris-Cl,
pH 7.5).
¡ñ Plant genomic DNA concentration is determined by pico
green measurement while other genomic
•DNA concentration is determined by UV260 measurement.
¡ñ RNase treatment to ensure the RNA contamination is
eliminated.
¡ñ Hind III digestion test: genomic DNA was successfully
digested by Hind III.
¡ñ β-Actin expression was tested by PCR amplification for
Genomic DNA or PCR ready genomic DNA. |
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