BioChain-Genomic DNA,cDNA,RNA,Protein,Antibody,PCR,Cells,Biomarkers,Reagent,Tissue

New Products

DNA Methylation Detection Kit (Nov. 2008)
BioChain's DNA methylation detection kit offers a fast, efficient and convenient alternative to traditional bisulfite conversions. Our kit provides users with a true endogenous human positive control which eliminates any ambiguity in validating results. The positive control has been tested in over 15 different human tissues. We have optimized our protocol to minimize DNA degradation, reagent preparation, and conversion time. Our protocol guarantees > 99 % conversion of cytosine to uracil and > 99 % CpG protection, as calculated by sequencing, and is sensitive over a range of 2 µg - 500 pg of DNA. In less than 3 hours, users can obtain high quality bisulfite modified DNA ready for analysis by PCR, sequencing, or microarrays.

FFPE Tissue DNA Extraction Kit (Nov. 2008)
BioChain's FFPE Tissue DNA Extraction Kit allows for facile and efficient deoxyribonucleic acid extraction from FFPE tissues. All necessary reagents for DNA extractions in FFPE tissue specimens are provided, including FFPE tissue lysis buffer, proteinase K, and an actin control primer for control qPCR reactions. The kit contains sufficient reagents for 100 FFPE tissue DNA extraction reactions.

FFPE Tissue RNA Extraction Kit (Nov. 2008)
BioChain's FFPE Tissue RNA Extraction Kit allows for facile and efficient ribonucleic acid extraction from FFPE tissues. All necessary reagents for RNA extractions in FFPE tissue specimens are provided, including FFPE tissue lysis buffer, proteinase K, and an actin control primer for control RT-qPCR reactions. The kit contains sufficient reagents for 100 FFPE tissue RNA extraction reactions.

Blood DNA Isolation Kit (Nov. 2008)
BioChain's Blood DNA Isolation Kit offers a fast, efficient, and convenient alternative to traditional phenol or protease treatment. Our protocol guarantees isolate DNA from 50 µl to 200 µl whole blood sample. In less than 15 minutes, users can obtain pure DNA (>20kb) ready for analysis by PCR, sequencing, or microarrays.

Serum DNA Isolation Kit (Nov. 2008)
BioChain's Serum DNA Isolation kit offers a fast, efficient, and convenient alternative to traditional phenol or protease treatment. Our protocol guarantees isolate DNA from 200 µl serum or plasma samples. In less than 15 minutes, users can obtain pure DNA ready for analysis by PCR, sequencing, or microarrays.

Calcium Channel Cell Lines (Oct. 2008)
Voltage-gated calcium channels control both electrical signal transduction and intracellular calcium level. They can be generally classified into three families, designated as CaV1~3 families. T-type Ca2+ channels, or low-voltage-activated calcium channels, belong to the CaV3 family ion channels. They are activated upon minute voltage changes near the resting membrane potential. Their activation has been shown to mediate cardiac action potential, neuronal rebound burst firing and synchronization of thalamic activity; their dysfunction has been associated with various neurological and cardiovascular disorders. Their interaction with potential antagonists and inhibitors has been associated with pharmacological treatments for pain and epilepsy.

Rodent Custom Services (Oct. 2008)
BioChain has completed the establishment of a rodent facility in the San Francisco Bay area. Our facility is currently hosting major strains of inbred and outbred mice, rats, and hamsters. Our facility is fully AAALAC accredited. With our state-of-the-art facility and experienced scientists and technicians, we are well-positioned as a premium service provider in contract animal research. BioChain Institute is dedicated to providing our clients a wide range of preclinical contract animal research services designed to support various biological and pharmaceutical studies. These services range from pharmacokinetics, toxicology studies to the complete outsourcing of entire animal studies.

Mouse Feeder Cells (Sep. 2008)
Biochain provides mouse embryonic fibroblast (MEF) cells as feeder layers for stem cell culture to the research community. We have a wide range of selection of MEF cells including neomycin resistant, DR4 (resistant to neomycin, hygromycin, puromycin and 6-thioguanine) and CF-1 MEF cells. We have both untreated or gamma irradiated MEF cells. Every single batch of MEF cells is tested for its ability to support undifferentiated mESC growth and is mycoplasma negative. Early passages (P2) are provided for further expansion if more cells are needed.

Xenograft Service (Sep. 2008)
Living Tumor xenograft models are developed using human tumor tissues grafted in subrenal capsules of SCID mice. The human tumor tissues are developed into transplantable tumor tissue lines. These tumor tissue lines offer a sustainable tumor source for xenograft models. The model is in many aspects superior to conventional tumor model systems which are based on cultured cancer cell lines. There are over 100 established tumor lines available from Biochain.

Primate Animal Model Services (Sep. 2008)
According to FDA regulations, all drug candidates for Neurological diseases, such as Alzheimer's disease and Parkinson's disease, must pass nonhuman primate animal tests before processing into clinical trials. In addition, all cell-based therapies and gene-therapies are also required to go through nonhuman primate animal tests before advancing to a phase I clinical trial. Nonhuman primate animal models need to be established for the research of the following important human diseases, such as AIDS, SARS, autoimmune system diseases, old age diseases, and other gene defective diseases. Biochain has teamed up with contract research organizations (CROs), which are fully accredited by AAALAC international, and exclusively focus on primate resources. We have a very strong team of scientists, researchers, veterinarians, and technicians, who are actively engaged in developing nonhuman primate models for studies of drug safety, drug pharmacokinetics, drug efficacy, and cell and gene therapies, particularly for neurological disorders and aging-related diseases.

Prostate Tumor Frozen Tissue Array (Mar. 2008)
This frozen tissue array consists of 24 different prostate tumors and 4 corresponding normal controls. BioChain's human tumor frozen tissue arrays are designed for studying tumor tissue specific cellular localization of genes or proteins of interest in different human tissues. 37 same kind of tumors and 3 corresponding normal tissues are mounted on positively charged glass slides. Total RNA and cDNA from those tissues may also be available upon request.

Endothelial Progenitor Cell (EPC) (Mar. 2008)
The endothelial progenitor cell (EPC) is a primitive cell type in the endothelial lineage. They are bone marrow derived cells with properties similar to those of embryonic angioblasts. These progenitor cells migrate into the blood stream and are able to differentiate into a variety of mature vascular endothelial cell types. EPC play an important role in both angiogenesis and vasculogenesis. Compared to specific mature types of endothelial cells such as HMVEC and HUVEC, EPC are more suitable for cell-based screening of anti-cancer drugs. In addition, EPC have impact on aging and smoking-related diseases, suggesting potential uses of EPC in these areas.

Tumor Frozen Tissue Array (Feb. 2008)
BioChain's human tumor frozen tissue arrays are designed for studying tumor tissue specific cellular localization of genes or proteins of interest in different human tissues. 37 same kind of tumors and 3 corresponding normal tissues are mounted on positively charged glass slides. Total RNA and cDNA from those tissues may also be available upon request.

Lateral Flow Assay Kits (Jan. 2008)
A lateral flow test typically incorporates a sample pad that is intimately associated with a conjugate pad. This in turn touches a membrane onto which test and control reagents have been immobilised. An absorbent pad wicks fluid away from the membrane. Using lateral flow assay system, many kits can be developed for rapit diagnosis.

Anti-HEV IgG ELISA Kit (Jan. 2008)
This kit uses indirect ELISA method to detect anti-HEV antibodies IgG in serum or plasma. The purified activated recombinant HEV antigen (ORF2?ORF3) is coated on the multi-wells plate. The HRP conjugated mouse anti human IgG (r chain) monoclonal antibody serves as tracer. TMB is substrate for HRP. The enzyme reaction with substrate TMB produces a color change, and the intensity of the absorbance at 450 nm indicates the presence or absence of Anti-HEV antibodies IgG in the sample. The test is specific, sensitive, reproducible and easy to operate. It is for blood screen of HEV infection.

Anti-HEV IgM ELISA Kit (Jan. 2008)
This kit uses Capture ELISA method to detect anti-HEV antibodies IgM in serum or plasma. The mouse anti human IgM (?chain) monoclonal antibody is coated on the multi-wells plate. The HRP conjugated recombinant HEV antigen serves as tracer. TMB is substrate for HRP. The enzyme reaction with substrate TMB produces a color change, and the intensity of the absorbance at 450 nm indicates the presence or absence of Anti-HEV antibodies IgM in the sample. The test is specific, sensitive, reproducible and easy to operate. It is for blood screen of HEV infection.

TORCH ELISA Kits (Jan. 2008)
Biochain has developed a series of ELISA kits for TORCH test of Toxoplasmosis, CMV, Rubella, HSV. TORCH Stands for the initial letters of the five infectious-disease categories, which are toxoplasmosis, other infections, rubella, cytomegalovirus (CMV), and herpes simplex virus (HSV). The "other infections" usually include syphilis, hepatitis B, coxsackie virus, Epstein-Barr virus, varicella-zoster virus, and human parvovirus. TORCH test measures the levels of an infant's antibodies against these five groups of chronic infections. Biochain has developed a series of ELISA kits for TORCH test of Toxoplasmosis, CMV, Rubella, HSV.

EasyXpress Viral Nucleic Acid Release Kit (Jan. 2008)
The EasyXpress Viral Nucleic Release Kit is designed for the rapid isolation of small quantities of viral RNA or DNA from cell culture supernatants and biological samples in microfuge tubes or in a 96-well format for subsequent quantitative and real-time RT-PCR analysis. The kit contains VNAR Protease, a proprietary mixture of buffers and proteases that allow for the isolation of up to 96 viral nucleic acid samples in under an hour with no sample filtration or precipitation steps.

TeloExpress Quantitative Telomerase Detection Kit (Jan. 2008)
The Telomerase Detection Kit is an optimized and validated real-time PCR Kit for the quantification of human telomerase activity that can be utilized in most real-time PCR platforms. In this telomeric repeat amplification protocol (TRAP), the telomerase activity in cell extracts adds the telomeric (TTAGGG) repeat sequences to the 3?end of an oligonucleotide substrate. The number of repeat sequences added by the enzyme is quantified using real-time PCR by measuring the increase in SYBR green fluorescence upon binding to DNA. Real-time PCR is conducted under conditions in which primer dimer formation is negligible and the extent of repeat amplification is directly proportional to telomerase activity.

EZ-Blood RNA 96 Kit (Jan. 2008)
The EZ-Blood RNA 96 kit is designed for the high-throughput purification of total cellular RNA from whole blood in a 96-well plate format. No phenol-chloroform extraction or precipitation steps are involved. The sample addition and washing steps are performed using any 96-well compatible vacuum manifold, while the lysis of red blood cells (RBCs) and the final elution of the RNA product are performed using a table-top centrifuge.

EZ-Blood DNA 96 Kit (Jan. 2008)
The EZ-Blood DNA 96 Kit is designed for the high-throughput purification of genomic DNA from whole blood in a 96-well plate format. No phenol-chloroform extraction or precipitation steps are involved. The sample addition and washing steps are performed using any 96-well compatible vacuum manifold, while the lysis of red blood cells (RBCs) and the final elution of the DNA product are performed using a table-top centrifuge. The red blood cells (RBCs) in whole blood are first selectively lysed and the while blood cells are pelleted by centrifugation.

EZ-RNA 96 Kit (Jan. 2008)
EZ-RNA 96 Kit is designed for the high-throughput purification of total cellular RNA from cultured cells in a 96-well plate format. No phenol-chloroform extraction or precipitation steps are involved. The kit can also be used to cleanup up to 96 RNA samples at a time.

EZ-DNA 96 Kit (Jan. 2008)
EZ-DNA 96 Kit is designed for the high-throughput purification of genomic DNA from cultured cells in a 96-well plate format. No phenol-chloroform extraction or precipitation steps are involved. The kit can also be used to cleanup up to 96 DNA samples at a time.