New
Products
DNA Methylation Detection Kit
(Nov. 2008)
BioChain's
DNA methylation detection kit offers a fast, efficient and
convenient alternative to traditional bisulfite conversions. Our
kit provides users with a true endogenous human positive control
which eliminates any ambiguity in validating results. The
positive control has been tested in over 15 different human
tissues. We have optimized our protocol to minimize DNA
degradation, reagent preparation, and conversion time. Our
protocol guarantees > 99 % conversion of cytosine to uracil and
> 99 % CpG protection, as calculated by sequencing, and is
sensitive over a range of 2 µg - 500
pg of DNA. In less than 3 hours, users can obtain high quality
bisulfite modified DNA ready for analysis by PCR, sequencing, or
microarrays.
FFPE Tissue DNA Extraction Kit
(Nov. 2008)
BioChain's
FFPE Tissue DNA Extraction Kit allows for facile and efficient
deoxyribonucleic acid extraction from FFPE tissues. All
necessary reagents for DNA extractions in FFPE tissue specimens
are provided, including FFPE tissue lysis buffer, proteinase K,
and an actin control primer for control qPCR reactions. The kit
contains sufficient reagents for 100 FFPE tissue DNA extraction
reactions.
FFPE
Tissue RNA Extraction Kit
(Nov. 2008)
BioChain's
FFPE Tissue RNA Extraction Kit allows for facile and efficient
ribonucleic acid extraction from FFPE tissues. All necessary
reagents for RNA extractions in FFPE tissue specimens are
provided, including FFPE tissue lysis buffer, proteinase K, and
an actin control primer for control RT-qPCR reactions. The kit
contains sufficient reagents for 100 FFPE tissue RNA extraction
reactions.
Blood DNA Isolation Kit
(Nov. 2008)
BioChain's
Blood DNA Isolation Kit offers a fast, efficient, and convenient
alternative to traditional phenol or protease treatment. Our
protocol guarantees isolate DNA from 50 µl to 200 µl whole blood
sample. In less than 15 minutes, users can obtain pure DNA
(>20kb) ready for analysis by PCR, sequencing, or microarrays.
Serum DNA Isolation Kit
(Nov. 2008)
BioChain's
Serum DNA Isolation kit offers a fast, efficient, and convenient
alternative to traditional phenol or protease treatment. Our
protocol guarantees isolate DNA from 200 µl serum or plasma
samples. In less than 15 minutes, users can obtain pure DNA
ready for analysis by PCR, sequencing, or microarrays.
Calcium Channel Cell Lines (Oct. 2008)
Voltage-gated calcium channels control both
electrical signal transduction and intracellular calcium level.
They can be generally classified into three families, designated
as CaV1~3 families. T-type Ca2+ channels, or
low-voltage-activated calcium channels, belong to the CaV3
family ion channels. They are activated upon minute voltage
changes near the resting membrane potential. Their activation
has been shown to mediate cardiac action potential, neuronal
rebound burst firing and synchronization of thalamic activity;
their dysfunction has been associated with various neurological
and cardiovascular disorders. Their interaction with potential
antagonists and inhibitors has been associated with
pharmacological treatments for pain and epilepsy.
Rodent Custom Services (Oct. 2008)
BioChain has completed the establishment of a
rodent facility in the San Francisco Bay area. Our facility is
currently hosting major strains of inbred and outbred mice,
rats, and hamsters. Our facility is fully AAALAC accredited.
With our state-of-the-art facility and experienced scientists
and technicians, we are well-positioned as a premium service
provider in contract animal research. BioChain Institute is
dedicated to providing our clients a wide range of preclinical
contract animal research services designed to support various
biological and pharmaceutical studies. These services range from
pharmacokinetics, toxicology studies to the complete outsourcing
of entire animal studies.
Mouse Feeder
Cells (Sep. 2008)
Biochain provides mouse
embryonic fibroblast (MEF) cells as feeder layers for stem cell
culture to the research community. We have a wide range of
selection of MEF cells including neomycin resistant, DR4
(resistant to neomycin, hygromycin, puromycin and 6-thioguanine)
and CF-1 MEF cells. We have both untreated or gamma irradiated
MEF cells. Every single batch of MEF cells is tested for its
ability to support undifferentiated mESC growth and is
mycoplasma negative. Early passages (P2) are provided for
further expansion if more cells are needed.
Xenograft
Service (Sep. 2008)
Living
Tumor xenograft models are developed using human tumor tissues
grafted in subrenal capsules of SCID mice. The human tumor
tissues are developed into transplantable tumor tissue lines.
These tumor tissue lines offer a sustainable tumor source for
xenograft models. The model is in many aspects superior to
conventional tumor model systems which are based on cultured
cancer cell lines. There are over 100 established tumor lines
available from Biochain.
Primate
Animal Model Services (Sep. 2008)
According
to FDA regulations, all drug candidates for Neurological
diseases, such as Alzheimer's disease and Parkinson's disease,
must pass nonhuman primate animal tests before processing into
clinical trials. In addition, all cell-based therapies and
gene-therapies are also required to go through nonhuman primate
animal tests before advancing to a phase I clinical trial.
Nonhuman primate animal models need to be established for the
research of the following important human diseases, such as
AIDS, SARS, autoimmune system diseases, old age diseases, and
other gene defective diseases. Biochain has teamed up with
contract research organizations (CROs), which are fully
accredited by AAALAC international, and exclusively focus on
primate resources. We have a very strong team of scientists,
researchers, veterinarians, and technicians, who are actively
engaged in developing nonhuman primate models for studies of
drug safety, drug pharmacokinetics, drug efficacy, and cell and
gene therapies, particularly for neurological disorders and
aging-related diseases.
Prostate Tumor
Frozen Tissue Array (Mar. 2008)
This
frozen tissue array consists of 24 different prostate tumors and
4 corresponding normal controls. BioChain's human tumor
frozen tissue arrays are designed for studying tumor tissue
specific cellular localization of genes or proteins of interest
in different human tissues. 37 same kind of tumors and 3
corresponding normal tissues are mounted on positively charged
glass slides. Total RNA and cDNA from those tissues may also be
available upon request.
Endothelial
Progenitor Cell (EPC) (Mar. 2008)
The
endothelial progenitor cell (EPC) is a primitive cell type in
the endothelial lineage. They are bone marrow derived cells with
properties similar to those of embryonic angioblasts. These
progenitor cells migrate into the blood stream and are able to
differentiate into a variety of mature vascular endothelial cell
types. EPC play an important role in both angiogenesis and
vasculogenesis. Compared to specific mature types of endothelial
cells such as HMVEC and HUVEC, EPC are more suitable for
cell-based screening of anti-cancer drugs. In addition, EPC have
impact on aging and smoking-related diseases, suggesting
potential uses of EPC in these areas.
Tumor Frozen Tissue Array
(Feb. 2008)
BioChain's human tumor
frozen tissue arrays are designed for studying tumor tissue
specific cellular localization of genes or proteins of interest
in different human tissues. 37 same kind of tumors and 3
corresponding normal tissues are mounted on positively charged
glass slides. Total RNA and cDNA from those tissues may also be
available upon request.
Lateral Flow Assay Kits
(Jan. 2008)
A
lateral flow test typically incorporates a sample pad that is
intimately associated with a conjugate pad. This in turn touches
a membrane onto which test and control reagents have been
immobilised. An absorbent pad wicks fluid away from the
membrane. Using lateral flow assay system, many kits can be
developed for rapit diagnosis.
Anti-HEV IgG ELISA Kit (Jan. 2008)
This kit uses indirect
ELISA method to detect anti-HEV antibodies IgG in serum or
plasma. The purified activated recombinant HEV antigen
(ORF2?ORF3) is coated on the multi-wells plate. The HRP
conjugated mouse anti human IgG (r chain) monoclonal antibody
serves as tracer. TMB is substrate for HRP. The enzyme reaction
with substrate TMB produces a color change, and the intensity of
the absorbance at 450 nm indicates the presence or absence of
Anti-HEV antibodies IgG in the sample. The test is specific,
sensitive, reproducible and easy to operate. It is for blood
screen of HEV infection.
Anti-HEV IgM ELISA Kit
(Jan. 2008)
This kit uses Capture
ELISA method to detect anti-HEV antibodies IgM in serum or
plasma. The mouse anti human IgM (?chain) monoclonal antibody
is coated on the multi-wells plate. The HRP conjugated
recombinant HEV antigen serves as tracer. TMB is substrate for
HRP. The enzyme reaction with substrate TMB produces a color
change, and the intensity of the absorbance at 450 nm indicates
the presence or absence of Anti-HEV antibodies IgM in the
sample. The test is specific, sensitive, reproducible and easy
to operate. It is for blood screen of HEV infection.
TORCH ELISA Kits
(Jan. 2008)
Biochain has developed a
series of ELISA kits for TORCH test of Toxoplasmosis, CMV,
Rubella, HSV. TORCH Stands for the initial letters of the five
infectious-disease categories, which are toxoplasmosis, other
infections, rubella, cytomegalovirus (CMV), and herpes simplex
virus (HSV). The "other infections" usually include syphilis,
hepatitis B, coxsackie virus, Epstein-Barr virus, varicella-zoster
virus, and human parvovirus. TORCH test measures the levels of
an infant's antibodies against these five groups of chronic
infections. Biochain has developed a series of ELISA kits for
TORCH test of Toxoplasmosis, CMV, Rubella, HSV.
EasyXpress Viral Nucleic Acid Release Kit
(Jan. 2008)
The EasyXpress Viral
Nucleic Release Kit is designed for the rapid isolation of small
quantities of viral RNA or DNA from cell culture supernatants
and biological samples in microfuge tubes or in a 96-well format
for subsequent quantitative and real-time RT-PCR analysis. The
kit contains VNAR Protease, a proprietary mixture of buffers and
proteases that allow for the isolation of up to 96 viral nucleic
acid samples in under an hour with no sample filtration or
precipitation steps.
TeloExpress Quantitative Telomerase Detection Kit
(Jan. 2008)
The Telomerase Detection
Kit is an optimized and validated real-time PCR Kit for the
quantification of human telomerase activity that can be utilized
in most real-time PCR platforms. In this telomeric repeat
amplification protocol (TRAP), the telomerase activity in cell
extracts adds the telomeric (TTAGGG) repeat sequences to the
3?end of an oligonucleotide substrate. The number of repeat
sequences added by the enzyme is quantified using real-time PCR
by measuring the increase in SYBR green fluorescence upon
binding to DNA. Real-time PCR is conducted under conditions in
which primer dimer formation is negligible and the extent of
repeat amplification is directly proportional to telomerase
activity.
EZ-Blood RNA 96 Kit
(Jan. 2008)
The EZ-Blood RNA 96 kit
is designed for the high-throughput purification of total
cellular RNA from whole blood in a 96-well plate format. No
phenol-chloroform extraction or precipitation steps are
involved. The sample addition and washing steps are performed
using any 96-well compatible vacuum manifold, while the lysis of
red blood cells (RBCs) and the final elution of the RNA product
are performed using a table-top centrifuge.
EZ-Blood DNA 96 Kit
(Jan. 2008)
The EZ-Blood DNA 96 Kit
is designed for the high-throughput purification of genomic DNA
from whole blood in a 96-well plate format. No phenol-chloroform
extraction or precipitation steps are involved. The sample
addition and washing steps are performed using any 96-well
compatible vacuum manifold, while the lysis of red blood cells (RBCs)
and the final elution of the DNA product are performed using a
table-top centrifuge. The red blood cells (RBCs) in whole blood
are first selectively lysed and the while blood cells are
pelleted by centrifugation.
EZ-RNA 96 Kit
(Jan. 2008)
EZ-RNA 96 Kit is
designed for the high-throughput purification of total cellular
RNA from cultured cells in a 96-well plate format. No
phenol-chloroform extraction or precipitation steps are
involved. The kit can also be used to cleanup up to 96 RNA
samples at a time.
EZ-DNA 96 Kit
(Jan. 2008)
EZ-DNA 96 Kit is
designed for the high-throughput purification of genomic DNA
from cultured cells in a 96-well plate format. No
phenol-chloroform extraction or precipitation steps are
involved. The kit can also be used to cleanup up to 96 DNA
samples at a time.