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Matched Pair - cDNA
Human cDNA matched pair products include: Primary Pair (PP),
Primary and Metastatic Pair (PM). PP consists of cDNAs
isolated from primary tumor and its adjacent normal tissue;
PM consists of cDNAs from primary tumor and corresponding
metastatic tumor. cDNAs in each pair are prepared from the
same donor. This product line is designed for identifying
tumor-specific genes and tumor metastatic genes.
Quality Controls
¡ñ The integrity of
the RNA used for cDNA synthesis is examined by visual
inspection for the presence of intact bands of 18s and 28s
ribosomal RNA when electrophoreses on a denaturing agarose
gel. The quality and purity of total RNA were tested by
spectrophotometer. A260/280 is between 1.8 and 2.0 (detected
in 10 mM Tris-Cl, pH 7.5). The ratio of 28S/18S is ≥1.
¡ñ The RNA used for cDNA synthesis is treated by DNase I,
and is tested as DNA free RNA by PCR.
¡ñ The synthesized human, animal, and cell line cDNA was
selected to ensure its full length. The cDNA was used as
template for PCR amplification of β-actin gene and an 838 bp
β-actin band was visualized on 1% agarose gel. β-actin
control primer is included. It is enough for 10 PCR
reactions.
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