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Gene Amplification and Cloning
Polymerase chain reaction (PCR) is
widely used technique gene amplification. The
method relies on thermal cycling, primers (short DNA
fragments) containing sequences complementary to the
target region along with a DNA polymerase (after
which the method is named) are key components to
enable selective and repeated amplification. As PCR
progresses, the DNA template is exponentially
amplified.
Related Kits and Reagents:
Total RNA,
mRNA,
reverse transcriptase,
cDNA,
cDNA library,
genomic DNA,
Taq polymerase, oligo nucleotide, and
dNTP, etc.
Molecular cloning refers to the process of
making multiple copies of a defined DNA sequence.
Cloning is frequently used to amplify DNA fragments
containing whole genes, but it can also be used to
amplify any DNA sequence such as promoters,
non-coding sequences and randomly fragmented DNA. It
is used in a wide array of biological experiments
and practical applications ranging from genetic
fingerprinting to large scale protein production.
Related Kits and Reagents: cloning vectors,
expression vectors, restriction enzyme,
modification enzymes,
plasmid preparation kit, DNA gel purification kit,
etc. |
First Strand cDNA
The largest cDNA gene pools
from a wide variety of biological samples |
cDNA Panel
cDNA combination for analyzing gene expression patterns and
discovering novel genes |
cDNA Library
Premade cDNA libraries from
unique sources, such as Xenopus, Stem cells |
Total RNA
Total RNA from hard-to-obtain
tissues. Each Total RNA sample is prepared with our
proprietary method. Our rigorous quality control tests
ensure each preparation consists of intact RNA with
virtually no genomic DNA contamination. |
mRNA
mRNA from hard-to-obtain
tissues. each mRNA sample is enriched for mRNA transcripts
with two rounds of oligo(dT)-cellulose purification. |
Genomic DNA
The largest source for
epigenetic studies, including DNA methylation, SNP, and DNA
biomarker discovery |
Small Size Genomic DNA
Unique template for
digital PCR |
Cloning Checker
The fastest way to confirm
your cloning vector construction |
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