Frozen Tissue Section
Features
¡ñ
Better
antigen exposure than Paraffin tissue sections
¡ñ
Simpler detection procedure
¡ñ
Duplicated tissues or a single tissue per slide
¡ñ
Easier
extraction of better quality DNA, RNA or protein
Applications
¡ñ
Cellular localization of tissue specific mRNA and protein
expression
¡ñ
Identification of tumor and disease specific genes, proteins
by in situ hybridization, immunohistochemistry, and in situ
PCR detection
¡ñ
Comparison of the locations of novel genes in different
tissues Control slides for immunohistochemistry studies
Quality
Control
¡ñ
At
least one of the tissue slides from each lot was stained
with H & E to ensure the quality
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