First Strand cDNA
Features
¡ñ Ready to use for
PCR
¡ñ Oligo dT primer used to ensure the entire 3' end of cDNA is
present
¡ñ
With some cDNA used as templates, 12 kb PCR amplicon was
obtained to ensure the intact cDNAs
¡ñ
The largest selection of cDNAs from different tissues on the
market
¡ñ
Documentation of tissues' clinical histories available
Applications
¡ñ Gene cloning and
target sequencing
¡ñ
Immediate PCR Amplification of known genes
¡ñ
Verification of genetic mutation
¡ñ
Comparison of a specific gene between different tissues
¡ñ
Analysis of mRNA alternative splicing
Quality Controls
¡ñ The integrity of
the RNA used for cDNA synthesis is examined by visual
inspection for the presence of intact bands of 18s and 28s
ribosomal RNA when electrophoreses on a denaturing agarose
gel. The quality and purity of total RNA were tested by
spectrophotometer. A260/280 is between 1.8 and 2.0 (detected
in 10 mM Tris-Cl, pH 7.5). The ratio of 28S/18S is ≥1.
¡ñ The RNA used for cDNA synthesis is treated by DNase I,
and is tested as DNA free RNA by PCR.
¡ñ The synthesized human, animal, and cell line cDNA was
selected to ensure its full length. The cDNA was used as
template for PCR amplification of β-actin gene and an 838 bp
β-actin band was visualized on 1% agarose gel. β-actin
control primer is included. It is enough for 10 PCR
reactions.
¡ñ The synthesized plant cDNA was used as template for PCR
amplification of chloroplast gene. A 458 bp chloroplast band
was visualized on 1% agarose gel. Chloroplast control primer
is included. It is enough for 10 PCR reactions. |
