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Why Frozen Tissue is Better Than Paraffin Embedded Tissue
        
Intensity of IHC Comparison between Frozen and Paraffin Sections
Neuronal cells and fibers in the PVN of a paraffin section are shown in C and in a frozen section are shown in E. ....The staining of fibers was less intense in paraffin section. –KURIYAMA, et al. Endocrinology 145 (5): 2542-2550
 
Intensity of ISH Comparison between Frozen and Paraffin Sections
Comparison of signal intensities of hybridized VIP mRNA in mouse brain cortex on cryostat sections (A and C) and paraffin sections (B and D). Results from both 33P labeled (A and B) and digoxigenin-labeled probes (C and D) are shown. Note that intensities of hybridized signals on frozen tissue sections (A and C) are much stronger than those on the paraffin sections (B and D). -Chris Carlson at el, Optimizing In Situ Hybridization Protocols
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