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Why Frozen Tissue
is Better Than Paraffin Embedded Tissue |
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Intensity of IHC Comparison between
Frozen and Paraffin Sections
Neuronal cells and fibers
in the PVN of a paraffin section are
shown in C and in a frozen section are
shown in E. ....The staining of fibers was
less intense in paraffin section.
–KURIYAMA,
et al. Endocrinology 145 (5): 2542-2550
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Intensity of ISH Comparison between Frozen
and Paraffin Sections
Comparison of
signal intensities of
hybridized VIP mRNA in mouse brain cortex on
cryostat sections (A and C) and paraffin
sections (B and D). Results from both 33P
labeled (A and B) and digoxigenin-labeled probes
(C and D) are shown. Note that intensities of
hybridized signals on frozen tissue sections
(A and C) are much stronger than those
on the paraffin sections (B and D). -Chris
Carlson at el, Optimizing In Situ Hybridization
Protocols
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