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Figure
10.1 SDS-PAGE analysis of human normal
and tumor tissue protein samples
Lane1:
Breast;
Lane2: Breast tumor;
Lane3: Colon; Lane4: Colon tumor;
Lane5: Liver; Lane6: Liver tumor;
Lane7: Lung; Lane8: Lung tumor.
Each lane contains 40 µg of protein
approximately
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Figure
10.2 Western blotting analysis of separation
of cytoplasmic (C), nuclear (N) and
membrane (M) proteins from normal lung
and lung tumor tissues.
Panel A: Anti-ß-actin mouse monoclonal
antibody was used to localize cytoplasmic
protein.
Panel B: Anti-EGFR goat polyclonal antibody
was used to localize membrane protein.
The EGFR signal of tumor tissue is much
stronger than that of normal tissue.
Approximately 40 µg of protein was loaded
per lane. |
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